A5009103795- Plant Virus Research Studies
- Plant Pathogenic Bacteria Studies
- Insect-Plant Interactions and Control
- Plant and Fungal Interactions Research
- Plant-Microbe Interactions and Immunity
- Cocoa and Sweet Potato Agronomy
- Plant Disease Management Techniques
- Plant Disease Resistance and Genetics
- Insect and Pesticide Research
- Transgenic Plants and Applications
- Banana Cultivation and Research
- Insect symbiosis and bacterial influences
- Insect Resistance and Genetics
- Agronomic Practices and Intercropping Systems
- Phytoplasmas and Hemiptera pathogens
- Plant tissue culture and regeneration
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Plant Parasitism and Resistance
- Viral gastroenteritis research and epidemiology
- Ecology and Vegetation Dynamics Studies
- Plant Pathogens and Resistance
- Growth and nutrition in plants
- CRISPR and Genetic Engineering
- Botanical Research and Applications
Brazilian Agricultural Research Corporation
2015-2024
Universidade de Brasília
2013-2024
Horta (Italy)
2005-2024
Universidade Federal de Viçosa
2012
Universidade de São Paulo
2011
Agricultural Research Corporation
2006
Universidade Católica de Brasília
2004
Graduate School Experimental Plant Sciences
1998
The family Potyviridae includes plant viruses with single-stranded, positive-sense RNA genomes of 8-11 kb and flexuous filamentous particles 650-950 nm long 11-20 wide. Genera in the are distinguished by host range, genomic features phylogeny member viruses. Most monopartite, but those members genus Bymovirus bipartite. Some cause serious disease epidemics cultivated plants. This is a summary International Committee on Taxonomy Viruses (ICTV) Report Potyviridae, which available at...
Tomato severe rugose virus (ToSRV) is the most important begomovirus species in Brazilian tomato production. Many weeds are associated with tomato, and some hosts of begomoviruses. Only one weed, Nicandra physaloides, has been found to be infected ToSRV. In this study, four weed were investigated for their capacity by ToSRV serve as a potential source inoculum tomato. Begomoviruses from naturally Crotalaria spp., Euphorbia heterophylla, N. Sida spp. successfully transferred plants biolistic...
Although the Sw-5 gene cluster has been cloned, and Sw-5b identified as functional copy that confers resistance to Tomato spotted wilt virus (TSWV), its avirulence (Avr) determinant not date. Nicotiana tabacum 'SR1' plants transformed with a of are immune without producing clear visual response on challenge TSWV, whereas it is shown here N. benthamiana gives rapid conspicuous hypersensitive (HR). Using these plants, from all structural non-structural TSWV proteins tested, cell-to-cell...
The development of tomato spotted wilt tospovirus (TSWV) infection in the midgut and salivary glands transmitting non-transmitting thrips, Frankliniella occidentalis, was studied to elucidate tissue tropism virus pathway within body this vector. Immunohistological techniques used study showed that midgut, foregut were only organs which accumulated. first signals infection, observed as randomly distributed fluorescent granular spots, found epithelial cells mainly restricted anterior region....
Tospoviruses (Genus Tospovirus, Family Bunyaviridae) are phytopathogens responsible for significant worldwide crop losses. They have a tripartite negative and ambisense RNA genome segments, termed S (Small), M (Medium) L (Large) RNA. The vector-transmission is mediated by thrips in circulative-propagative manner. For new tospovirus species acceptance, several analyses needed, e.g., the determination of viral protein sequences enlightenment their evolutionary history.Biological (host range...
A non-transgenic approach based on RNA interference was employed to induce protection against tomato mosaic virus (ToMV) infection in plants. dsRNA molecules targeting the cp gene of ToMV were topically applied plants prior inoculation. Protection dose-dependent and sequence-specific. While no achieved when 0-16 µg used, maximum rates resistance (60 63%) observed doses 200 400 µg/plant, respectively. Similar also obtained potato Y its gene. The quickly activated upon application lasted for...
Abstract The severe yellowing disease ( amarelão ) on melon plants is a serious problem in Brazil, although the causative agent remained unknown for long time. Recently, recombinant isolates of cucurbit aphid‐borne yellows virus (CABYV) were reported as possible agents this plants. Although aphids are known to be vectors common type CABYV isolates, almost no aphid colony was observed major fields Brazil with high incidence disease. In contrast, whiteflies often abundant. Based observation,...
The competence of a Frankliniella occidentalis and Thrips tabaci population to transmit Tomato spotted wilt virus (TSWV) was analysed. Adults the F. transmitted this efficiently, whereas those thelytokous T. failed transmit. TSWV replicated in midgut larvae both populations after ingestion virus; however, lower amounts accumulated than larvae. almost undetectable adults, high titres were readily detected adults. first infections by immunocytochemical studies epithelial subsequently muscle...
Summary Begomovirus‐associated epidemics currently threaten tomato production worldwide due to the emergence of highly pathogenic virus species and proliferation a whitefly B biotype vector that is adapted tomato. To generate an efficient defence against begomovirus, we modulated activity immune receptor nuclear shuttle protein ( NSP )‐interacting kinase NIK ) in plants; virulence target begomovirus during infection. Mutation T474 within activation loop promoted constitutive ‐mediated...
ABSTRACT Tomato chlorotic mottle virus (ToCMoV) is an emerging begomovirus species widely distributed throughout tomato-growing regions of Brazil. ToCMoV appears to have expanded its geographic range recently, invading areas that were free infection before 2004. We determined the first complete genome sequence infectious (isolate BA-Se1), which isolated in northeast When introduced by particle bombardment into tomato, cloned ToCMoV-[BA-Se1] DNA-A and DNA-B components caused typical symptoms....
Abstract Background Monopartite begomoviruses (genus Begomovirus , family Geminiviridae ) that infect sweet potato ( Ipomoea batatas around the world are known as sweepoviruses. Because plants vegetatively propagated, accumulation of viruses can become a major constraint for root production. Mixed infections sweepovirus species and strains lead to recombination, which may contribute generation new recombinant Results This study reports full genome sequence 34 sweepoviruses sampled from...
Two defective RNA-containing isolates (Pe-1 and 16-2) an envelope-deficient (env ) isolate of Tomato spotted wilt virus (TSWV) were tested for their transmissibility by Frankliniella occidentalis. The Pe-1 contained a truncated L RNA segment that barely interfered with symptom expression replication the wild-type (wt) segment. This was transmitted efficiency 51%, value comparable to found wt TSWV (54%). Isolate 16-2, which genuine interfering as concluded from its ability suppress synthesis...