A5051215749- Glaucoma and retinal disorders
- Retinal Development and Disorders
- Telomeres, Telomerase, and Senescence
- Retinal Diseases and Treatments
- RNA regulation and disease
- Corneal surgery and disorders
- Retinal and Macular Surgery
- Retinal and Optic Conditions
Tohoku University
2018-2024
Purpose: Glutamate excitotoxicity seems to contribute retinal ganglion cell (RGC) death in various eye diseases, but the underlying molecular mechanisms are not fully understood. We studied roles of cyclin-dependent kinase inhibitors Cdkn2a and Cdkn2b, known as cellular stress-related senescence markers, N-methyl-d-aspartate (NMDA)-induced RGC death. Methods: Gene expression was analyzed using quantitative reverse transcription (qRT)-PCR, situ hybridization, immunochemistry. Cdkn2b gain-...
Purpose: To assess the therapeutic potential of endothelin-converting enzyme-like 1 (Ecel1) in a mouse model optic nerve crush. Methods: Ecel1 expression was evaluated with real time quantitative (qRT)-PCR, Western blotting, and immunohistochemistry retinas after Vinblastine administration to intravitreal injection N-methyl-d-aspartate (NMDA) were used gene expression. deleted an adeno-associated viral (AAV) clustered regulatory interspaced short palindromic repeat (CRISPR)/Cas9 system,...
Abstract Glaucoma is a leading cause of blindness worldwide in older people. Profiling the aqueous humor, including metabolites it contains, useful to understand physiological and pathological conditions eye. In current study, we used mass spectrometry (MS) characterize humor metabolomic profile biological features patients with glaucoma. Aqueous samples were collected during trabeculectomy surgery or cataract analyzed global metabolomics. We included 40 glaucoma (32 POAG, 8 NTG) 37 control...