We report the fabrication of an amperometric whole-cell herbicide biosensor based on magnetic retention living cells functionalised with nanoparticles (MNPs) surface a screen-printed electrode. demonstrate that Chlorella pyrenoidosa microalgae coated biocompatible MNPs and retained electrode permanent magnet act as sensing element for fast detection herbicides. The functionalisation does not affect viability photosynthesis activity-mediated triazine recognition in microalgae. current...

Electrochemical enzyme biosensors are extensively utilized in clinical analysis and environmental monitoring, yet achieving effective immobilization while maintaining high activity remains a challenge. In this work, we developed flow-through biosensor system using 3D-printed electrochemical cell fabricated from commercially available poly (lactic acid). After modification with thiacalixarene-functionalized oligo acids) (OLAs), the material enabled efficient of uricase on inner surface...

The global shift toward personalized medicine and point-of-care testing drives the need for improved analytical performance in sensor technologies. A critical aspect of developing voltammetric sensors lies identifying novel...

Whether the spring season brings additional pollution to urban environment remains questionable for a megacity. Aerosol sampling and characterization was performed in background of Moscow megacity 2017, period significant impact mass advection from surrounding fire regions. Parametrization Angstrom absorption exponent (AAE) on low high values provides periods dominated by fossil fuel (FF) combustion affected biomass burning (BB), respectively. The identification is supported air...

Neurodegenerative diseases and Alzheimer’s disease (AD), as one of the most common causes dementia, result in progressive losses cholinergic neurons a reduction presynaptic markers system. These consequences can be compensated by inhibition acetylcholinesterase (AChE) followed decrease rate acetylcholine hydrolysis. For this reason, anticholinesterase drugs with reversible effects are applied for administration neurodegenerative diseases. Their overdosage, variation efficiency recommendation...

A flow-through biosensor system for the determination of uric acid was developed on platform electrochemical cell manufactured by 3D printing from poly(lactic acid) and equipped with a modified screen-printed graphite electrode (SPE). Uricase immobilized to inner surface replaceable reactor chamber. Its working volume reduced 10 μL against previously reported similar cell. SPE independently enzyme carbon black, pillar[5]arene, poly(amidoamine) dendrimers based p-tert-butylthiacalix[4]arene...

Abstract The biosensors based on DNA aptamers immobilized by electrostatic adsorption onto electropolymerized Methylene Green (MG)) imprinted with have been developed and examined for thrombin detection in the range from 1 nM to μM using electrochemical impedance spectroscopy (EIS) potentiometry. participation of MG electropolymerization imprints formation was proved measurements EIS redox signals neutral charged indicators. Changes morphology layers were atomic force microscopy (AFM)....

Abstract The effect of aptamer structure and immobilization platform on the efficiency thrombin binding its detection using electrochemical impedance spectroscopy (EIS) characteristics was investigated with aptasensors based glassy carbon electrodes covered multiwalled nanotubes (MWNTs). Aptamers one or two sequences GGTTGGTGTGGTTGG specific for poly(dA) poly(dT) tags able to form dimeric products (aptabodies) were used establish significance steric electrostatic factors in aptasensor...

Novel DNA-sensor with enzymatic amplification of the signal has beendeveloped on base glassy carbon electrode modified ds-DNA and horseradishperoxidase (HRP). Phenothiazine dyes Methylene Blue Green were used aselectrochemical markers for detection sulfonamide anthracycline preparationsable to interact DNA. The biosensor related HRP oxidation markersdepends relation between their bonded readily oxidized forms which depends onthe nature concentration pharmaceuticals. Sulfonamides diminish...

Abstract Oxidative damage of DNA was assessed with glassy carbon electrode (GCE) coated electropolymerized Methylene Blue (MB). For this purpose, solution first mixed an oxidant (Fenton reagent, H 2 O alone and in the presence Cu(II) ions) then placed on polymeric film surface. After washing, cyclic voltammogram recorded buffer anodic peak potential measured against that before contact DNA. resulted remarkable decrease which depended nature incubation period. Specificity – MB interactions...

The paradigm of drug delivery via nano- and microcarriers is one the leading ideas that enable overcoming limitations traditional chemotherapy. trend toward more complex carriers capable multifunctionality observed in literature. To date, prospects stimuli-responsive systems to control cargo release lesion nidus are widely accepted. Both endogenous exogenous stimuli employed for this purpose, however, pH most common triggers. Unfortunately, scientists face difficulties implementation idea...

A cholinesterase potentiometric sensor based on a glassy carbon electrode modified with processible polyaniline has been developed and explored for the detection of organophosphorus carbamic pesticides. Acetyl- butyryl-cholinesterase from various sources were immobilized surface by cross-linking glutaraldehyde. Polyaniline modification made it possible to obtain reversible pH response about 86-mV/pH unit improve analytical operational characteristics substrate inhibitor detection. The...

Abstract New acetylcholinesterase biosensors have been developed on the base of screen‐printed electrodes modified with Co‐phtalocyanine and polycarboxylated thiacalixarenes as enzyme support. The showed improved operational analytical characteristics high accuracy signal measurement. made it possible to detect 3–200 ppb paraoxon 1–50 malaoxon detection limits down 2 0.05 ppb, respectively (incubation time: 15 min). influence macrocyclic receptors was related nonenzymatic accumulation...

Abstract The performance of potentiometric sensors based on carbon screen‐printed and glassy electrodes covered with polyaniline (PANI) thiacalix[4]arene receptors have been compared in the determination some ions discrimination green tea brands. optimization PANI electropolymerization provided satisfactory characteristics comparable those conventional carbon. make it possible to determine 1×10 −2 –1×10 −6 M Fe 3+ ions. Also, successful twelve brands was performed three‐sensor array. 100 %...

Abstract An electrochemical immunosensor based on indirect competitive ELISA technique has been developed and tested for the detection of azinphos‐methyl in aqueous solutions spiked honeybee extracts. The pesticide was competition binding to monoclonal antibodies with an ovalbumin (OVA) conjugate, followed by incubation anti‐mouse IgG labeled horseradish peroxidase, whose activity measured amperometrically hydroquinone as substrate. sensitivity assay, estimated IC50 value, found be 1.2 nmol...

A flow-through biosensor has been developed and manufactured by 3D printing from poly(lactic acid). Uricase or tyrosinase were immobilized on the inner walls of reactor cell concentrations reaction products, i.e., hydrogen peroxide in case uricase quinone product tyrosine oxidation tyrosinase, monitored amperometric mode. The screen-printed electrode modified with carbon black, pillar[5]arene electropolymerized thionine methylene blue was separated enzyme layer 0.1 mm gap used as a...