A5089526901- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Biochemical and biochemical processes
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant-derived Lignans Synthesis and Bioactivity
- Legume Nitrogen Fixing Symbiosis
- Bioactive natural compounds
- Phytochemicals and Antioxidant Activities
- Genomics, phytochemicals, and oxidative stress
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Essential Oils and Antimicrobial Activity
- Algal biology and biofuel production
- Allelopathy and phytotoxic interactions
- Plant-Microbe Interactions and Immunity
- Plant Molecular Biology Research
- Polyamine Metabolism and Applications
- Epigenetics and DNA Methylation
- Prenatal Substance Exposure Effects
- Adenosine and Purinergic Signaling
- Subcritical and Supercritical Water Processes
- Aquatic Ecosystems and Phytoplankton Dynamics
- Phytoplasmas and Hemiptera pathogens
- Traditional and Medicinal Uses of Annonaceae
Washington State University
2015-2024
Philipps University of Marburg
2005-2013
Plants live in association with microorganisms that positively influence plant development, vigor, and fitness response to pathogens abiotic stressors. The bulk of the microbiome is concentrated belowground at root-soil interface. Plant roots secrete carbon-rich rhizodeposits containing primary secondary low molecular weight metabolites, lysates, mucilages. These exudates provide nutrients for soil modulate their affinity host plants, but details this process are largely unresolved. We...
Polymethoxylated flavonoids occur in a number of plant families, including the Lamiaceae. To date, metabolic pathways giving rise to diversity these compounds have not been studied. Analysis our expressed sequence tag database for four sweet basil (Ocimum basilicum) lines afforded identification candidate flavonoid O-methyltransferase genes. Recombinant proteins displayed distinct substrate preferences and product specificities that can account all detected 7-/6-/4'-methylated,...
Salvia divinorum (Lamiaceae) is an annual herb used by indigenous cultures of Mexico for medicinal and ritual purposes. The biosynthesis salvinorin A, its major bioactive neo-clerodane diterpenoid, remains virtually unknown. This investigation aimed to identify the enzyme that catalyzes first reaction A biosynthesis, formation (-)-kolavenyl diphosphate [(-)-KPP], which subsequently dephosphorylated afford (-)-kolavenol. Peltate glandular trichomes were identified as perhaps exclusive site...
Potato ( Solanum tuberosum L ) is affected by several viral pathogens with the most economically damaging being potato virus Y (PVY). At least nine biologically distinct variants of PVY are known to attack potato, necrotic types named NTN and N-Wi recent additions list. So far, molecular plant-virus interactions underlying this pathogenicity not fully understood. In study, gas chromatography coupled mass spectroscopy (GC–MS) was used for an untargeted investigation changes in leaf...
As cannabis use increases among reproductive-aged women, there is a growing need to better understand the presence of cannabinoids in milk produced by women using cannabis. It unclear how concentrations such as delta-9-tetrahydrocannabinol (Δ
Most elucidated hydroxylations in plant secondary metabolism are catalyzed by oxoglutarate- or cytochrome P450-dependent oxygenases. Numerous still evade clarification, suggesting that they might be performed alternative enzyme types. Here, we report the identification of flavone 8-hydroxylase (F8H) sweet basil (Ocimum basilicum L.) trichomes as a Rieske-type oxygenase. Several features F8H activity trichome protein extracts helped to differentiate it from P450-catalyzed reaction and...
Bay laurel (Laurus nobilis L.) is an agriculturally important tree used in food, drugs, and the cosmetics industry. Many of health beneficial properties bay are due to volatile terpene metabolites that they contain, including various norisoprenoids. Despite their importance, little known about norisoprenoid biosynthesis Laurus fruits. We found norisoprenoids 6-methyl-5-hepten-2-one, pseudoionone, β-ionone accumulated fruits a pattern reflecting carotenoid content. A full-length cDNA encoding...
Dear Editor, Extracellular ATP (eATP) is a purinergic signal recognized by plasma membrane-localized transmembrane receptors, such as P2X or P2Y receptors found in mammals (Verkhratsky and Burnstock, 2014), the P2K plants (Choi et al., 2014; Pham 2020). In mammals, eATP purinoceptors are basis of intercellular signaling used to regulate diverse processes including neuronal signaling, apoptosis, inflammation 2014). plants, P2K1 appear influence root growth (Weerasinghe 2009; Zhu 2017), but...
Mercaptoethane sulfonate or coenzyme M (CoM) is the smallest known organic cofactor and most commonly associated with methane-forming step in all methanogenic archaea but also anaerobic oxidation of methane to CO 2 methanotrophic short-chain alkanes Syntrophoarchaeum species. It has been found a small number bacteria capable metabolism organics. Although many steps for CoM biosynthesis have elucidated, complete pathway not reported. Here, we present bacteria, revealing distinct chemical...
Rootstock selection and crop load adjustment are key practices in apple orchard management; nevertheless, the effects of rootstocks levels on important physiological processes scions, such as photosynthetic performance carbohydrate accumulation, still unclear. To investigate impact different scion photosynthesis buildup, 2020, 'Honeycrisp' trees grafted 'G.41', 'G.935', 'M.9-T337' were thinned to low high levels, accumulation leaves fruit evaluated. Leaves from 'G.935' showed highest net...
Small molecule demethylation is considered unusual in plants. Of the studied instances, N-demethylation of nicotine catalyzed by a Cyt P450 monooxygenase, while O-dealkylation alkaloids Papaver somniferum mediated 2-oxoglutarate-dependent dioxygenases (2-ODDs). This report describes 2-ODD regiospecifically catalyzing 7-O-demethylation methoxylated flavones peltate trichomes sweet basil (Ocimum basilicum L.). Three candidate 2-ODDs were identified trichome transcriptome database. Only...
Plants deploy a variety of chemical and physical defenses to protect themselves against herbivores pathogens. Organic farming seeks enhance these responses by improving soil quality, ultimately altering bottom up regulation plant defenses. While laboratory studies suggest this approach is effective, it remains unclear whether organic agriculture encourages more-active under real-world conditions. Working on the farms cooperating growers, we examined gene expression in leaves two potato...
Salicylic acid (SA) is a phytohormone that plays manifold roles in plant growth, defense, and other aspects of physiology. The concentration free SA plants fine-tuned by variety structural modifications. produced all land plants, yet it not known whether its metabolism conserved lineages. Selaginella moellendorffii lycophyte thus representative an ancient clade vascular plants. Here, we evaluated the accumulation related metabolites aerial parts S. moellendorffii. We found primarily stored...
Coniferyl alcohol 9-O-methyltransferase from Linum nodiflorum (Linaceae) catalyzes the unusual methylation of side-chain hydroxyl group coniferyl alcohol. The protein was heterologously expressed in Escherichia coli as a hexahistidine derivative and purified for crystallization. Diffracting crystals were obtained pure its selenomethionine derivative, well complexes with S-adenosyl-l-homocysteine together 9-O-methyl ether (PDB entries 4ems, 4e70 4evi, respectively). X-ray structures show that...
Summary ‘ Candidatus Liberibacter asiaticus ’ is a fastidious bacterium and putative agent of citrus greening disease (a.k.a., huanglongbing, HLB), significant agricultural that affects fruit quality tree health. In citrus, Ca. L. phloem limited. Lack culture tools to study complicates analysis this important organism. To improve understanding ’–host interactions including parameters affect replication, methods suitable for screening pathogen responses physicochemical nutritional variables...