A5056171837- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Photoreceptor and optogenetics research
- Algal biology and biofuel production
- Plant Molecular Biology Research
- Spectroscopy and Quantum Chemical Studies
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Renal and related cancers
- Liver Disease Diagnosis and Treatment
- Neuroblastoma Research and Treatments
- Advanced Fluorescence Microscopy Techniques
- Physiological and biochemical adaptations
- Genetic Mapping and Diversity in Plants and Animals
- Inflammatory mediators and NSAID effects
- 3D Printing in Biomedical Research
- Cytomegalovirus and herpesvirus research
- Endoplasmic Reticulum Stress and Disease
- Genetic and Kidney Cyst Diseases
- Metal complexes synthesis and properties
- Telomeres, Telomerase, and Senescence
- Histone Deacetylase Inhibitors Research
- Tissue Engineering and Regenerative Medicine
- Academic Publishing and Open Access
University of Colorado Anschutz Medical Campus
2025
University of Glasgow
2014-2024
Friedrich Schiller University Jena
2018-2024
Asklepios Klinik St. Georg
2013
Institute for Interdisciplinary Medicine
2013
University of Bonn
2011-2012
Universität Hamburg
2002-2008
University Medical Center Hamburg-Eppendorf
2006-2008
Monash University
2005
Leibniz Institute of Virology (LIV)
2002
Speeding up stomatal responses A plant's cellular metabolism rapidly adjusts to changes in light conditions, but its stomata—pores that allow gas exchange leaves—are slower respond. Because of the lagging response, photosynthesis is less efficient, and excess water lost through open pores. Papanatsiou et al. introduced a blue light–responsive ion channel into stomata small mustard plant Arabidopsis. The increased rate opening closing response light. engineered plants produced more biomass,...
An optogenetic tool to silence neurons Potassium channels in the cell membrane open and close response molecular signals alter local potential. Cosentino et al. linked a light-responsive module pore of potassium channel build genetically encoded called BLINK1 that is closed dark opens low doses blue light. Zebrafish embryos expressing their changed behavior Science , this issue p. 707
Abstract Exposure of plants to ultraviolet-B (UV-B) radiation initiates transcriptional responses that modify metabolism, physiology and development enhance viability in sunlight. Many these regulatory UV-B are mediated by the photoreceptor UV RESISTANCE LOCUS 8 (UVR8). Following photoreception, UVR8 interacts directly with multiple proteins regulate gene expression, but mechanisms control differential protein binding initiate distinct unknown. Here we show is phosphorylated at several sites...
Circadian clocks govern temporal programs in the green lineage (Chloroplastida) as they do other photosynthetic pro- and eukaryotes, bacteria, fungi, animals, humans. Their physiological properties, including entrainment, phase responses, temperature compensation, are well conserved. The involvement of transcriptional/translational feedback loops oscillatory machinery reversible phosphorylation events also maintained. control a large variety output rhythms algae terrestrial plants, adjusting...
Significance A key challenge for plant molecular biologists is to increase yield by altering photosynthetic productivity secure food, energy, and environmental sustainability. In the model Arabidopsis thaliana , plasma-membrane–associated phototropin kinases, phot1 phot2, are activated blue light play important roles in regulating several responses that optimize efficiency. However, little effort has been made target these pathways growth. Here, we demonstrate modifying photocycle of phot2...
The late embryogenesis abundant (LEA)-like protein CDeT11-24 is one of the major desiccation-related phosphoproteins resurrection plant Craterostigma plantagineum. In this study, it was shown that mostly intrinsically disordered and protects two different enzymes, citrate synthase lactate dehydrogenase, against damaging effects caused by desiccation. Lipid-binding assays revealed able to interact with phosphatidic acid, although electrostatic repulsion expected due overall negative net...
Lipid peroxidation is one of the consequences environmental stress in plants and leads to accumulation highly toxic, reactive aldehydes. One processes detoxify these aldehydes their oxidation into carboxylic acids catalyzed by NAD(P)+-dependent ALDHs (aldehyde dehydrogenases). We investigated kinetic parameters two Arabidopsis thaliana family 3 ALDHs, cytosolic ALDH3H1 chloroplastic isoform ALDH3I1. Both enzymes had similar substrate specificity oxidized saturated aliphatic Catalytic...
Summary Drosophila , Arabidopsis Synechocystis Homo (DASH) cryptochromes belong to the cryptochrome/photolyase family and can act as DNA repair enzymes. In bacteria fungi, they also play regulatory roles, but in plants their biological functions remain elusive. Here, we characterize CRY‐DASH1 from green alga Chlamydomonas reinhardtii . We perform biochemical vitro photochemical analysis. For functional characterization, a knock‐out mutant of cry‐dash1 is used. protein localized chloroplast...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEPR characterization of the CP47-D1-D2-cytochrome b-559 complex photosystem IIJan Petersen, Jan P. Dekker, Neil R. Bowlby, Demetrios F. Ghanotakis, Charles Yocum, and Gerald T. BabcockCite this: Biochemistry 1990, 29, 13, 3226–3231Publication Date (Print):April 3, 1990Publication History Published online1 May 2002Published inissue 3 April 1990https://pubs.acs.org/doi/10.1021/bi00465a012https://doi.org/10.1021/bi00465a012research-articleACS...
Summary Phototropins (phots) regulate a range of adaptive processes in plants that serve to optimize photosynthetic efficiency and promote growth. Light sensing by Arabidopsis thaliana phots is predominantly mediated the Light, Oxygen Voltage 2 ( LOV 2) flavin‐binding motif located within N‐terminus photoreceptor. Here we characterize photochemical biochemical properties phot from marine picoalga Ostreococcus tauri phototropin (Otphot) examine its ability replace phot‐mediated function ....
Phototropins (phots) are plasma membrane-associated serine/threonine kinases that coordinate a range of processes linked to optimizing photosynthetic efficiency in plants. These photoreceptors contain two light-, oxygen-, or voltage-sensing (LOV) domains within their N terminus, with each binding one molecule flavin mononucleotide as UV/blue light-absorbing chromophore. Although phots LOV domains, light-induced activation the C-terminal kinase domain and subsequent receptor...
Plant cryptochromes are central blue light receptors in land plants and algae. Photoreduction of the flavin bound to photolyase homology region (PHR) causes a dissociation C-terminal extension (CCT) as effector via an unclear pathway. We applied recently developed in-cell infrared difference (ICIRD) spectroscopy study response full-length pCRY from Chlamydomonas reinhardtii living bacterial cells, because receptor degraded upon isolation. demonstrate stabilization neutral radical...
Protein kinases (PKs) control many aspects of plant physiology by regulating signaling networks through protein phosphorylation. Phototropins (phots) are plasma membrane–associated serine/threonine PKs that a range physiological processes collectively serve to optimize photosynthetic efficiency in plants. These include phototropism, leaf positioning and flattening, chloroplast movement, stomatal opening. Despite their identification over two decades ago, only handful substrates have been...
Algae encode up to five different types of cryptochrome photoreceptors. So far, relatively little is known about the biological functions DASH (Drosophila, Arabidopsis, Synechocystis and Homo)-type cryptochromes. The green alga Chlamydomonas reinhardtii encodes two them. CRY-DASH1 also called DCRY1 has its maximal absorption peak in UV-A range. It localized chloroplast plays an important role balancing photosynthetic machinery. Here, we performed a comparative analysis proteins from wild...
Microalgae contribute significantly to carbon fixation on Earth. Global warming influences their physiology and growth rates. To understand algal short-term acclimation adaptation changes in ambient temperature, it is essential identify characterize the molecular components that sense small temperature as well downstream signaling networks physiological responses. Here, we used green biflagellate alga Chlamydomonas reinhardtii a model system which study responses temperature. We report an...
Guanosine and 7-β-D-ribofuranosyltheophylline was oxidized to the corresponding nucleoside 5′-carboxylic acids by molecular oxygen a platinum catalyst.
Summary Photosynthetic protists, named microalgae, are key players in global primary production. The green microalga Chlamydomonas reinhardtii is a well‐studied model organism. In nature, it dwells acetate‐rich paddy rice soil, which not mimicked by standard liquid laboratory conditions. Here, we maintained the algae environment with spatially structured 3‐D components (S3‐D) and acetate recreating natural We perform transcriptome sequencing, immunoblotting, fluorescence electron microscopy,...