A5080959653- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- Neuroscience and Neural Engineering
- Bone Tissue Engineering Materials
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Cardiomyopathy and Myosin Studies
- 3D Printing in Biomedical Research
- Congenital heart defects research
- Viral Infections and Immunology Research
- Medical and Biological Ozone Research
- Orthopedic Infections and Treatments
- Graphene and Nanomaterials Applications
- Orthopaedic implants and arthroplasty
- Collagen: Extraction and Characterization
- Cardiac electrophysiology and arrhythmias
- Mesenchymal stem cell research
- biodegradable polymer synthesis and properties
- Bee Products Chemical Analysis
- Sinusitis and nasal conditions
University of Indonesia
2022-2024
University of Nottingham
2014-2020
Czech Academy of Sciences, Biology Centre
2019
Park University
2019
University of Lausanne
2019
Sarcomeric gene mutations frequently underlie hypertrophic cardiomyopathy (HCM), a prevalent and complex condition leading to left ventricle thickening heart dysfunction. We evaluated isogenic genome-edited human pluripotent stem cell-cardiomyocytes (hPSC-CM) for their validity model, add clarity to, HCM. CRISPR/Cas9 editing produced 11 variants of the HCM-causing mutation c.C9123T-MYH7 [(p.R453C-β-myosin heavy chain (MHC)] in 3 independent hPSC lines. Isogenic sets were differentiated...
Abstract Animal models are 78% accurate in determining whether drugs will alter contractility of the human heart. To evaluate suitability human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) for predictive safety pharmacology, we quantified changes contractility, voltage, and/or Ca2+ handling 2D monolayers or 3D engineered heart tissues (EHTs). Protocols were unified via a drug training set, allowing subsequent blinded multicenter evaluation with known positive, negative,...
Bone tissue engineering (BTE) is a promising alternative approach to the repair of damaged bone tissue. This study aims fabricate and characterize scaffolds composed chitosan (CS), hyaluronic acid (HA), hydroxyapatite (HAp), combination graphite (Gr), graphene oxide (GO), multi-walled carbon nanotubes (MWCNT) for BTE applications. The Gr MWCNT were functionalized by oxidation, while GO was synthesized using improved Hummer method. prepared lyophilization, physical, chemical, biological...
Recently, propolis has shown potential cardioprotective effects against myocardial infarction. However, challenges in its clinical application have arisen, primarily due to concerns regarding dosage and adverse effects. To address this, we suggest integrating into polyvinyl alcohol (PVA)/gelatin hydrogel regulate the localized release of at infarcted sites. PVA/gelatin hydrogels with varying concentrations (3%, 7%, 10%) were fabricated using a freeze–thawing method, characterized their...
Bone damage is one of the main causes disability in humans, and tissue engineering technology by applying biomaterial-based scaffold has been developed as an effective solution. This can be achieved using various natural synthetic polymers combined with carbon-based metal-oxide materials. Therefore,
Polyether–ether–ketone (PEEK) is a commonly employed biomaterial for spinal, cranial, and dental implant applications due to its mechanical properties, bio-stability, radiolucency, especially when compared metal alloys. However, biologically inert behavior poses substantial challenge in osseointegration between host bone PEEK implants, resulting loosening. Previous studies identified surface modification methods that prove beneficial enhancing stability supporting cell growth, but...
Abstract Purpose Cardiac tissue engineering opens up opportunities for regenerative therapy in heart diseases. Current technologies improve engineered cardiac characteristics by combining human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) with non-cardiomyocytes, selective biomaterials, and additional growth factors. Animal models are still required to determine patches’ overall vivo effect before initiating trials. Here, we review the current studies of patches using...
Bioreactors are growing in popularity among network engineers. generally chambers used for cell culture processes with predetermined parameters. Pivot is a bioreactor system that controlled automatically and remotely using multi-chambers allow inflow of fresh liquid or output sample collection. This facilitates gas exchange between the medium ambient gas. The provides storage bag media peristaltic pump to move closed loop during perfusion stirring chamber. flow rates 2 mL/min. has container...
Cardiomyocytes derived from human pluripotent stem cells (hPSC-CMs) provide a powerful tool for modelling impact of disease and drugs on structure function.This presentation will discuss three short stories around our research.First, modelling.Our early experiments investigated hiPSC-CMs carrying mutations that carry long QT syndrome allowed evaluation drug genetic therapies.We have subsequently created models hypertrophic cardiomyopathy by combining hiPSC, Cas9/CRISPR automated phenotyping...
Rhinosinusitis is a manifestation of the presence inflammation in nasal cavity and mucous membrane paranasal sinuses resulting formation fluid or damage to bone structures below. Radiological support examination cases rhinosinusitis can be done with conventional X-ray radiology sinuses, which combined computed tomography (CT) Magnetic Resonance Imaging (MRI) examination. This study compares traditional imaging, CT scanning, MRI patients chronic rhinosinusitis. A comprehensive literature...