A5005840330- Cellular transport and secretion
- Hippo pathway signaling and YAP/TAZ
- CRISPR and Genetic Engineering
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Multiple Myeloma Research and Treatments
- Retinal Development and Disorders
- Child Nutrition and Water Access
- Protein Kinase Regulation and GTPase Signaling
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Microtubule and mitosis dynamics
- Environmental Education and Sustainability
- Protein Degradation and Inhibitors
- PI3K/AKT/mTOR signaling in cancer
- Mitochondrial Function and Pathology
- interferon and immune responses
Salk Institute for Biological Studies
2019-2023
University of Dundee
2014-2019
MRC Protein Phosphorylation and Ubiquitylation Unit
2014-2019
Medical Research Council
2019
The Vps34 (vacuolar protein sorting 34) class III PI3K (phosphoinositide 3-kinase) phosphorylates PtdIns (phosphatidylinositol) at endosomal membranes to generate PtdIns(3)P that regulates membrane trafficking processes via its ability recruit a subset of proteins possessing PtdIns(3)P-binding PX (phox homology) and FYVE domains. In the present study, we describe highly selective potent inhibitor Vps34, termed VPS34-IN1, inhibits with 25 nM IC50 in vitro, but does not significantly inhibit...
Parkin is phosphorylated on Ser 108 in ACT domain by AMPK/ULK1 signaling within minutes of mitochondrial stress.
Cells respond to mitochondrial poisons with rapid activation of the adenosine monophosphate-activated protein kinase (AMPK), causing acute metabolic changes through phosphorylation and prolonged adaptation metabolism transcriptional effects. Transcription factor EB (TFEB) is a major effector AMPK that increases expression lysosome genes in response energetic stress, but how activates TFEB remains unresolved. We demonstrate directly phosphorylates five conserved serine residues...
Derailment of the PI3K-AGC protein kinase signalling network contributes to many human diseases including cancer. Recent work has revealed that poorly studied AGC family member, SGK3, promotes resistance cancer therapies target Class 1 PI3K pathway, by substituting for loss Akt activity. SGK3 is recruited and activated at endosomes, virtue its phox homology domain binding PtdIns(3)P. Here, we demonstrate endogenous rapidly growth factors such as IGF1, through pathways involving both 3 PI3Ks....
The serum- and glucocorticoid-regulated kinase (SGK) isoforms contribute resistance to cancer therapies targeting the PI3K pathway. SGKs are homologous Akt these kinases display overlapping specificity phosphorylate several substrates at same residues, such as TSC2 promote tumor growth by switching on mTORC1 SGK3 isoform is up-regulated in breast cells treated with or inhibitors recruited activated endosomes, through its phox homology domain binding PtdIns(3)P. We undertook genetic...
PLK (Polo-like kinase) inhibitors, such as BI-2536, have been reported to suppress IFNB (encoding IFNβ, interferon β) gene transcription induced by ligands that activate TLR3 (Toll-like receptor 3) and TLR4. In the present study, we found BI-2536 is likely exert this effect preventing interaction of factors IRF3 (interferon-regulatory factor c-Jun with promoter, but without affecting TBK1 {TANK [TRAF (tumour-necrosis-factor-receptor-associated factor)-associated nuclear κB activator]-binding...

 The main aim of the study was to assess knowledge, attitude and practices related environment particularly among female college students from first year fourth BS Home Economics. Both quantitative qualitative questions were included. 100 given questionnaires online, comprising both open close ended environmental water issues. Their responses showed a varying degree Knowledge, attitudes regarding these There significant difference in reported according grade level students. A higher...
Abstract The Vps34 Class III phosphoinositide 3-kinase (PI3K) phosphorylates phosphatidylinositol (PtdIns) at endosomal membranes to generate PtdIns(3)P that regulates membrane trafficking processes via its ability recruit a subset of proteins possessing PtdIns(3)P-binding PX and FYVE domains. Here we describe highly selective potent inhibitor Vps34, termed VPS34-IN1, inhibits with 25 nM IC50 in vitro but does not significantly inhibit the activity 340 protein kinases or lipid tested include...
Abstract The serum- and glucocorticoid-regulated kinase (SGK) isoforms contribute resistance to cancer therapies targeting the PI3K pathway. SGKs are homologous Akt these kinases display overlapping specificity phosphorylate several substrates at same residues, such as TSC2 promote tumor growth by switching on mTORC1 SGK3 isoform is upregulated in breast cells treated with or inhibitors recruited activated endosomes, through its phox homology domain binding PtdIns(3)P. We undertook genetic...

 Human health is the most important ingredient for a nation’s progress, prosperity and well-been but unfortunately it has not been accorded its due priority. Therefore, when going to deal with water of Lahore, was considered essential probe find out state quality. The main objective this article highlight microbial contamination drinking in selected localities Lahore. Furthermore, study will be helpful researchers administrative agencies initiate relevant studies develop new policies...