A5043904869- Immune Cell Function and Interaction
- Metabolism, Diabetes, and Cancer
- Inflammasome and immune disorders
- Neurogenetic and Muscular Disorders Research
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Plant Molecular Biology Research
- Immune cells in cancer
- Diet and metabolism studies
- Pancreatic function and diabetes
- Hair Growth and Disorders
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Autophagy in Disease and Therapy
- T-cell and B-cell Immunology
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer Mechanisms and Therapy
- Complement system in diseases
- Galectins and Cancer Biology
- Oral Health Pathology and Treatment
- Retinoids in leukemia and cellular processes
- Orthodontics and Dentofacial Orthopedics
- Alzheimer's disease research and treatments
- Cytomegalovirus and herpesvirus research
- Medicinal Plants and Bioactive Compounds
Salk Institute for Biological Studies
2019-2025
Case Western Reserve University
1994-2019
Peking University
2017-2019
Stomatology Hospital
2017
Cells respond to mitochondrial poisons with rapid activation of the adenosine monophosphate-activated protein kinase (AMPK), causing acute metabolic changes through phosphorylation and prolonged adaptation metabolism transcriptional effects. Transcription factor EB (TFEB) is a major effector AMPK that increases expression lysosome genes in response energetic stress, but how activates TFEB remains unresolved. We demonstrate directly phosphorylates five conserved serine residues...
The periderm provides a protective barrier in many seed plant species. development of the suberized phellem, which forms outermost layer this important tissue, has become trait interest for enhancing both resilience to stresses and plant-mediated CO
Despite being the frontline therapy for type 2 diabetes, mechanisms of action biguanide drug metformin are still discovered. In particular, detailed molecular interplays between AMPK and mTORC1 pathway in hepatic benefits ill defined. Metformin-dependent activation classically inhibits via TSC/RHEB, but several lines evidence suggest additional at play inhibition mTORC1. Here we investigated role direct AMPK-mediated serine phosphorylation RAPTOR a new Raptor AA mouse model, which...
Abstract Aim To investigate the effects of sodium butyrate (NaB) and lipopolysaccharide (LPS) on gingival epithelial barrier. Material methods We cultured human primary cells investigated NaB LPS barrier involved mechanisms at in vitro vivo levels by immunostaining, confocal microscopy, field emission scanning electron microscopy (FE‐SEM), transmission electronic (TEM), transepithelial electrical resistance (TEER), FTIC‐dextran flux, flow cytometry, real‐time PCR Western blot assays. Results...
The retinoic acid-related orphan receptor gamma (RORγt) acts as the major transcriptional activator in Th17 cell development and function to mediate adaptive immune defenses against pathogenic infection. RORγt engages accessible DNA response elements genome interplays with coactivator proteins accessory transcription factors drive gene expression. However, how chromatin environment mediates structure, dynamics, remains unclear. Here, we profile nucleosome promotes or restricts access main...
Abstract Defect in the SMN1 gene causes spinal muscular atrophy (SMA), which shows loss of motor neurons, muscle weakness and atrophy. While current treatment strategies, including small molecules or viral vectors, have shown promise improving function survival, achieving a definitive long-term correction SMA’s endogenous mutations phenotypes remains highly challenging. We previously developed CRISPR-Cas9 based homology-independent targeted integration (HITI) strategy, enabling...
Abstract Aging promotes numerous intracellular changes in T cells that impact their effector function. Our data show aging an increase the localization of STAT3 to mitochondria (mitoSTAT3), which mitochondrial dynamics and function T‐cell cytokine production. Mechanistically, mitoSTAT3 increased activity by increasing complex II. Limiting using a mitochondria‐targeted inhibitor, Mtcur‐1 lowered II activity, prevented age‐induced function, reduced Th17 inflammation. Exogenous expression...
Gene regulation is important for cells and tissues to function. It has been studied from two aspects at the genomic level, identification of expression quantitative trait loci (eQTLs) long-range chromatin interactions. understand their relationship, such as whether eQTLs regulate target genes through physical interaction. Although interactions have widely believed be one main mechanisms underlying eQTLs, most evidence came studies cell lines yet no direct exists tissues. We performed various...
Summary All organisms experience stress as an inevitable part of life, from single-celled microorganisms to complex multicellular beings. The ability recover is a fundamental trait that determines the overall resilience organism, yet recovery understudied. To begin unraveling process we studies drought in Arabidopsis thaliana . We performed fine-scale time series bulk RNA sequencing starting 15 minutes after rehydration following moderate drought. reveal rapid involving activation thousands...
db/db mice, which lack leptin receptors and exhibit hyperphagia, show disturbances in energy metabolism are a model of obesity type 2 diabetes. The geroneuroprotector drug candidate CMS121 has been shown to be effective animal models Alzheimer's disease aging through the modulation metabolism. Thus, hypothesis was that could protect mice from metabolic defects thereby reduce liver inflammation kidney damage. were treated with their diet for 6 months. No changes observed food oxygen...
Vitamin A/Retinoic Acid (Vit A/RA) signaling is essential for heart development. In cardiac progenitor cells (CPCs), RA induces the expression of atrial lineage genes while repressing ventricular genes, thereby promoting acquisition an cardiomyocyte cell fate. To achieve this, coordinates a complex regulatory network downstream effectors that not fully identified. address this gap, we applied functional genomics approach (i.e scRNAseq and snATACseq) to untreated RA-treated human embryonic...
Background: Modulated by differences in genetic and environmental factors, laboratory mice often show progressive weight gain, eventually leading to obesity metabolic dyshomeostasis. Since the geroneuroprotector CMS121 has a positive effect on energy metabolism mouse model of type 2 diabetes, we investigated potential counteract changes observed during ageing process wild mice. Methods: Control or CMS121-containing diets were supplied ad libitum for 6 months, sacrificed at age 7 months....
Abstract Regulatory T cells (T reg cells) play a critical role in suppressing anti-tumor immunity, often resulting unfavorable clinical outcomes across numerous cancers. However, systemic depletion, while augmenting responses, also triggers detrimental autoimmune disorders. Thus, dissecting the mechanisms by which navigate and exert their functions within tumor microenvironment (TME) is pivotal for devising innovative -centric cancer therapies. Our study highlights of peroxisome...
Abstract RAF kinase inhibitors can, in some conditions, increase signaling. This process, which is commonly referred to as “paradoxical activation” (PA), incompletely understood. kinases are regulated by autoinhibitory conformational changes, and the role of these changes PA unclear. Our mathematical investigations reveal that a dynamic equilibrium between autoinhibited non-autoinhibited forms RAF, along with inhibitor stabilization form, can be sufficient create PA. Using both computational...
Abstract Defect in the SMN1 gene causes spinal muscular atrophy (SMA), which shows loss of motor nerve cells, muscle weakness and atrophy. While current treatment strategies, including small molecules or viral vectors, have been reported to improve function survival, an ultimate long-term correct SMA endogenous mutations its phenotypes is still highly challenging. We previously developed a CRISPR-Cas9 based homology-independent targeted integration (HITI) strategy, allowed for unidirectional...