A5042069620- Atherosclerosis and Cardiovascular Diseases
- Cell Adhesion Molecules Research
- Single-cell and spatial transcriptomics
- Vasculitis and related conditions
- Angiogenesis and VEGF in Cancer
- Neuropeptides and Animal Physiology
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Neuroinflammation and Neurodegeneration Mechanisms
- Nuclear Receptors and Signaling
- Neurogenesis and neuroplasticity mechanisms
- Otitis Media and Relapsing Polychondritis
- Apelin-related biomedical research
- RNA Interference and Gene Delivery
Salk Institute for Biological Studies
2023-2025
University of California, San Diego
2023
Sanford Consortium for Regenerative Medicine
2023
Université de Limoges
2013-2014
Objective To identify the key coding genes underlying biomarkers and pathways associated with giant cell arteritis (GCA), we performed an in situ spatial profiling of molecules involved temporal arteries GCA patients controls. Furthermore, pharmacogenomic network analysis to potential treatment targets. Methods Using human formalin-fixed paraffin-embedded artery biopsy samples (GCA, n = 9; controls, 7), a whole transcriptome using NanoString GeoMx Digital Spatial Profiler. In total, 59...
Aging is associated with dysregulated methionine metabolism and increased levels of enzymes in the tyrosine degradation pathway (TDP). To investigate efficacy targeting either or TDP for healthspan improvement advanced age, we initiated dietary MetR inhibition 18-month-old C57BL/6J mice. significantly improved neuromuscular function, metabolic health, lung frailty. In addition, confirmed function from 5XFAD mice, whose weight was not affected by MetR. We did observe benefits inhibition....
Abstract Introduction Giant cell arteritis (GCA) is characterized by intimal hyperplasia leading to ischaemic manifestations that involve large vessels. Neurotrophins (NTs) and their receptors (NTRs) are protein factors for growth, differentiation survival of neurons. They also involved in the migration vascular smooth muscle cells (VSMCs). Our aim was investigate whether NTs NTRs remodelling GCA. Methods We included consecutive patients who underwent a temporal artery biopsy suspected...
Conventional type 1 dendritic cells (cDC1s) are critical for innate sensing of cancer, yet they scarce in the tumor microenvironment (TME). Here, we present a protocol to identify and isolate cDC1 subsets from murine implantable tumors subsequent transcriptomic profiling using flow sorting-based strategy. We describe steps cell culture mouse tumors, tumoral growth, dissociation isolation cells, extracellular staining, sorting. then detail procedures RNA isolation, mRNA library preparation,...
<h3>Background</h3> Giant cell arteritis (GCA) is the most common systemic vasculitis that triggers acute arterial occlusion and can even lead to death over age of 50 [1]. While mechanism underlying GCA are predominantly derived from inflammatory response, none anti-inflammatory drugs currently used limit efficiently patient's disease course. It was previously shown histopathological lesions involve three layers artery wall called adventitia, media, intima. Activation adventitial dendritic...