A5004365391- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Proteoglycans and glycosaminoglycans research
- Immune Cell Function and Interaction
- Toxoplasma gondii Research Studies
- Antimicrobial Peptides and Activities
- Cytomegalovirus and herpesvirus research
University of California, San Diego
2023-2025
University of California, Los Angeles
2022
Abstract Recent evidence has highlighted the critical requirement for “immune triads” of CD8+ T cells, CD4+ cells and dendritic (DC) tumor elimination in response to checkpoint inhibition (CPI) immunotherapy. However, mechanistic underpinnings this cross-talk are poorly understood. Immune triads often located peripherally-distributed DC-rich niches within stroma. We have hypothesized that stromal remodeling expansion generates homeostatic signals trigger an adaptive immune may ultimately...
Advances in immunotherapy the last decade have revolutionized treatment paradigms across multiple cancer diagnoses. However, only a minority of patients derive durable benefit and progress with traditional approaches, such as vaccines, remains unsatisfactory. A key to overcoming these barriers resides deeper understanding tumor antigen presentation complex dynamic heterogeneity tumor-infiltrating antigen-presenting cells (APCs). Reminiscent ‘second touch' hypothesis proposed by Klaus Ley for...
Papadas, A., Huang, Y., Cicala, Dou, Fields, M., Gibbons, Hong, D., Lagal, D.J., Quintana, V., Rizo, Zomalan, B. and Asimakopoulos, F. (2023) Emerging roles for tumor stroma in antigen presentation anti-cancer immunity. Biochem. Soc. Trans.51: 2017–2028. doi: https://doi.org/10.1042/BST20221083The authors would like to correct a passage of text from the "Dual non-cellular stromal actor: contrast between immunoregulatory proteoglycan versican versus its immunostimulatory proteolytic fragment,...
Abstract Tumor immune infiltration (“hot” tumor microenvironment, TME) constitutes a powerful predictor of outcomes and immunotherapy efficacy, however underlying mechanisms remain elusive. Recent evidence shows that TME-repriming incoming CD8+ T cells is required for full effector functionality. Reminiscent the “second-touch” hypothesis proposed by Klaus Ley CD4+ differentiation, tumor-draining lymph node (TDLN) priming (“first-touch”) generates antigen-specific stem-like TCF1+CD8+ cells....
Conventional type 1 dendritic cells (cDC1s) are critical for innate sensing of cancer, yet they scarce in the tumor microenvironment (TME). Here, we present a protocol to identify and isolate cDC1 subsets from murine implantable tumors subsequent transcriptomic profiling using flow sorting-based strategy. We describe steps cell culture mouse tumors, tumoral growth, dissociation isolation cells, extracellular staining, sorting. then detail procedures RNA isolation, mRNA library preparation,...
Intraperitoneal (i.p.) experimental models in mice can recapitulate the process of i.p. dissemination abdominal cancers and may help uncover critical information about future successful clinical treatments. cellular composition is studied preclinical addressing a wide spectrum other pathophysiological states such as liver cirrhosis, infectious disease, autoimmunity, aging. The peritoneal cavity multifaceted microenvironment that contains various immune cell populations, including T, B, NK,...