A5048487323- Sinusitis and nasal conditions
- Allergic Rhinitis and Sensitization
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- Head and Neck Surgical Oncology
- Hair Growth and Disorders
- Dermatology and Skin Diseases
- Nasal Surgery and Airway Studies
- Infectious Diseases and Mycology
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Oral and Maxillofacial Pathology
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
Eye & ENT Hospital of Fudan University
2017-2024
Salk Institute for Biological Studies
2022
Fudan University
2016-2019
Abstract RNA-binding proteins (RBPs) can recognize thousands of RNAs that help to maintain cell homeostasis, and RBP dysfunction is frequently observed in various cancers. However, whether specific RBPs are involved tumor immune evasion by regulating programmed death ligand-1 (PD-L1) unclear. Here, we perform targeted CRISPR/Cas9 screening identify density regulated re-initiation release factor (DENR) as a PD-L1 regulator. DENR-depleted cancer cells exhibit reduced expression vitro vivo....
Abstract Background IL ‐25 has been proposed to play a key role in the pathogenesis of chronic rhinosinusitis with nasal polyps ( CRS w NP ). This study aimed evaluate association Th2‐biased inflammatory profiles . Methods Nasal polyp ) tissues and control uncinate process were collected from 92 patients , 20 without s ), 16 normal subjects. expression was examined using immunohistochemistry immunofluorescence staining, flow cytometry, RT ‐ qPCR ELISA The clinical characteristics 2 subtypes...
Background: Allergic rhinitis is a chronic inflammatory and reactive disease of the nasal mucosa mediated by Immunoglobulin E (IgE) following exposure to allergens in atopic patients. The primary mediator involved histamine release, various immune-active cells cytokines contribute response. condition characterized itching, sneezing, hypersensitivity, swelling mucosa. Dust mites are prevalent China, both genetic environmental factors development allergic (AR). Discovering sensitizing crucial...
Purpose: Recent evidence suggests that B cells can both promote and inhibit the development progression of allergic disease.However, characteristics cell subsets in patients with rhinitis (AR) have not been well documented.This study aimed to analyze peripheral blood AR patients.Methods: Forty-seven 54 healthy controls were enrolled this study, all subjects analyzed by flow cytometry.Moreover, serum total immunoglobulin E (IgE) IgE concentrations secreted into cultured mononuclear (PBMCs)...
Periostin has been shown to be upregulated in chronic rhinosinusitis with nasal polyps (CRSwNP), especially the CRSwNP patients asthma. However, underlying mechanism that how periostin contributes polyp genesis remains unclear.In this study, we collected 63 patients' (NPs) and 25 control subjects' uncinated tissues. The expressions of periostin, thymic stromal lymphopoietin (TSLP), other proinflammatory cytokines were examined using IHC staining, qRT-PCR, Western blot (WB), ELISA FACS....
Abstract Objective To analyze the oxidative stress status and its association with tissue neutrophilia oral steroid response in chronic rhinosinusitis nasal polyps (CRSwNP) patients. Methods The levels of total oxidant (TOS) were detected sinonasal tissues by using specific assay kits. Tissue neutrophil was examined immunohistochemical staining, index (OSI) evaluated tissues, messenger RNA (mRNA) superoxide dismutase 2 (SOD2), aldehyde dehydrogenase 1 (ALDH1A1), microsomal glutathione...
The importance of IL-37 and downstream signal in the pathogenesis chronic rhinosinusitis with nasal polyps (CRSwNP) demanding further investigation.
Hrd1 has recently emerged as a critical regulator of B-cells in autoimmune diseases. However, its role the pathogenesis chronic rhinosinusitis with nasal polyps (CRSwNP) remains largely unexplored. This study aimed to examine expression and B-cell accumulation their possible roles CRSwNP.Quantitative real-time polymerase chain reaction, immunohistochemistry, enzyme-linked immunosorbent assay Western blotting were used assess gene protein tissue extracts. Cells isolated from tissues...
Background Decreased expression of airway epithelial‐specific transcription factor NK2 homeobox 1 (NKX2‐1) was associated with allergic inflammation in asthma patients. However, the and role NKX2‐1 nasal polyps (NPs) different endotypes were undefined yet. Methods We examined key cytokines (interleukin [IL]‐4 IL‐5, IL‐13, IL‐17A, etc.) NPs control tissues by immunohistochemistry staining, qualitative polymerase chain reaction (qPCR), enzyme‐linked immunosorbent assay (ELISA), Western blot...
PURPOSE Serum/glucocorticoid-regulated kinase 1 (SGK1) has recently emerged as a critical regulator of inflammatory diseases. In this study, we examined SGK1 expression and its possible pathogenic roles in chronic rhinosinusitis (CRS). METHODS Immunohistochemistry, western blotting, Bio-Plex assay, enzyme-linked immunosorbent assays, quantitative real-time polymerase chain reaction were performed to assess protein gene levels. The mRNA levels interleukin-6 (IL-6) extracted from CRS database...
Abstract Purpose To examine whether and how interleukin (IL)-1α is involved in chronic rhinosinusitis with nasal polyps (CRSwNP). Methods Nasal polyp (NP) control tissues were collected from CRSwNP patients subjects. The expression of IL-1α other proinflammatory cytokines (IL-1β, IL-8 IL-13, etc.), as well neutrophil eosinophil accumulation, examined sinonasal using immunohistochemical (IHC), immunofluorescent (IF) staining, qPCR, Luminex, respectively. Moreover, the regulation its effects...
Abstract The maintenance of tissue homeostasis in steady state or under stress is dependent on the proper communication between stem cells and supporting their microenvironment “niche”. In addition to promoting immune tolerance, regulatory T (Tregs) have recently emerged as a critical component cell niche hair follicle (HF), injured muscle, bone marrow, small intestine support differentiation maintain quiescence. How Treg sense dynamic signals environment communicate with during regeneration...