A5009558339- Biochemical and Molecular Research
- Protein Structure and Dynamics
- Photosynthetic Processes and Mechanisms
- Reproductive tract infections research
- Molecular spectroscopy and chirality
- Enzyme Structure and Function
- Research on Leishmaniasis Studies
- Monoclonal and Polyclonal Antibodies Research
- HIV/AIDS drug development and treatment
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Advanced NMR Techniques and Applications
- RNA and protein synthesis mechanisms
- Chemical Synthesis and Analysis
- Synthesis and Reactivity of Heterocycles
- Synthesis and Biological Evaluation
- Cyclopropane Reaction Mechanisms
- Reproductive Health and Contraception
- Protein Interaction Studies and Fluorescence Analysis
- Electron Spin Resonance Studies
- Various Chemistry Research Topics
- Hemoglobin structure and function
- Immune Response and Inflammation
- Carbohydrate Chemistry and Synthesis
- Free Radicals and Antioxidants
Adelphi University
2009-2023
Pfizer (United States)
2007-2010
Nerviano Medical Sciences
2002-2003
W.E. Upjohn Institute for Employment Research
1992-1999
Harvard University
1991-1992
University of Wisconsin–Madison
1988-1992
Sirtuins catalyze NAD(+)-dependent protein deacetylation and are critical regulators of transcription, apoptosis, metabolism, aging. There seven human sirtuins (SIRT1-7), SIRT1 has been implicated as a key mediator the pathways downstream calorie restriction that have shown to delay onset reduce incidence age-related diseases such type 2 diabetes. Increasing activity, either by transgenic overexpression Sirt1 gene in mice or pharmacological activation small molecule activators resveratrol...
Competition ligand-based NMR screening experiments have recently been introduced to overcome most of the problems associated with traditional screening. Molecules marginal solubility and high affinity for a given target can be easily identified in high-throughput manner by chemical mixtures against presence weak- medium-affinity ligand known binding constant. While original competition-based approaches utilized (1)H detection, significant advantages are obtained using (19)F detection. The...
Resistance to lincomycin and clindamycin in the clinical isolate Enterococcus faecium HM1025 is due a ribosomal methylase encoded by an ermAM-like gene plasmid-mediated inactivation of these antibiotics. We have cloned determined nucleotide sequence responsible for lincosamides, linB. This encodes 267-amino-acid lincosamide nucleotidyltransferase. The enzyme catalyzes 3(5'-adenylation) (the adenylation hydroxyl group position 3 molecules) clindamycin. Expression linB was observed both...
The Achilles heel of ligand-based NMR screening methods is their failure to detect high-affinity ligands and molecules that bind covalently the receptor. We have developed a novel approach for performing high-throughput with spectroscopy overcomes this limitation. method also permits detection potential are only marginally soluble, thus significantly enlarging diversity compounds amenable screening. techniques utilize transverse and/or selective longitudinal relaxation parameters in...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCorrelation of carbon-13 and nitrogen-15 chemical shifts in selectively uniformly labeled proteins by heteronuclear two-dimensional NMR spectroscopyWilliam M. Westler, Brian J. Stockman, Yoshiko. Hosoya, Yoko. Miyake, Masatsune. Kainosho, John L. MarkleyCite this: Am. Chem. Soc. 1988, 110, 18, 6256–6258Publication Date (Print):August 1, 1988Publication History Published online1 May 2002Published inissue 1 August...
Abstract The structure of the long‐chain flavodoxin from photosynthetic cyanobacterium Anabaena 7120 has been determined at 2 å resolution by molecular replacement method using atomic coordinates Anacystis nidulans . a third Chondrus crispus recently reported. Crystals oxidized A. belong to monoclinic space group P2 1 with = 48.0, b 32.0, c 51.6 å, and β 92°, one molecule in asymmetric unit. intensity data were collected oscillation films CHESS synchrotron source processed yield 9,795...
High-throughput ligand-based NMR screening with competition binding experiments is extended to 19F detection. Fluorine a favorable nucleus for these because of the significant contribution Chemical Shift Anisotropy (CSA) transverse relaxation ligand signal when bound macromolecular target. A low moderate affinity containing fluorine atom used as reference molecule detection and characterization new ligands. Titration selected compound are performed finding optimal set-up conditions HTS...
Aberrant activation of the phosphoinositide 3‐kinase pathway because genetic mutations essential signalling proteins has been associated with human diseases including cancer and diabetes. The pivotal role 3‐phosphoinositide‐dependent kinase‐1 in PI3K cascade made it an attractive target for therapeutic intervention. N‐terminal lobe catalytic domain contains a docking site which recognizes non‐catalytic C‐terminal hydrophobic motifs certain substrate kinases. binding this so‐called PDK1...
ABSTRACT The oxazolidinones are a novel class of antibiotics that inhibit initiation protein synthesis in bacteria. In order to investigate their mechanism action, the interactions several with bacterial 70S ribosomes, 50S subunits, and 30S subunits have been characterized by 1 H nuclear magnetic resonance (NMR) line-broadening analyses transferred Overhauser enhancement (TRNOE) experiments. PNU-177553 PNU-100592 (eperezolid) corresponding enantiomers, PNU-184414 PNU-107112, were studied....
When used in concert, one-bond carbon-carbon correlations, and multiple-bond proton-carbon proton-nitrogen derived from two-dimensional (2D) NMR spectra of isotopically enriched proteins, provide a reliable method assigning proton, carbon, nitrogen resonances. In contrast to procedures that simply extend proton assignments carbon or resonances, this technique assigns resonances coordinately on the basis their integrated coupling networks. Redundant spin pathways ways resolving overlaps...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFlavodoxin from Anabaena 7120: uniform nitrogen-15 enrichment and hydrogen-1, nitrogen-15, phosphorus-31 NMR investigations of the flavin mononucleotide binding site in reduced oxidized statesBrian J. Stockman, William M. Westler, Eddie S. Mooberry, John L. MarkleyCite this: Biochemistry 1988, 27, 1, 136–142Publication Date (Print):January 1988Publication History Published online1 May 2002Published inissue 1 January...
19F NMR-based methods have found utility in activity-based screening assays. However, because enzymes catalyze a diverse set of reactions, large variety fluorinated substrates would need to be identified target each one separately. We developed more streamlined approach that is applicable many utilize ATP as substrate. In this method, fluorine-containing analogue, 2-fluoro-ATP, used monitor the reaction. Applications are described for nicotinamide adenine dinucleotide synthetase and...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHydrogen-1, carbon-13, and nitrogen-15 NMR spectroscopy of Anabaena 7120 flavodoxin: assignment .beta.-sheet flavin binding site resonances analysis protein-flavin interactionsBrian J. Stockman, Andrzej M. Krezel, John L. Markley, Karin G. Leonhardt, Neil A. StrausCite this: Biochemistry 1990, 29, 41, 9600–9609Publication Date (Print):October 1, 1990Publication History Published online1 May 2002Published inissue 1 October...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSequence-specific proton and nitrogen-15 resonance assignments for human dihydrofolate reductase in solutionBrian J. Stockman, N. R. Nirmala, Gerhard Wagner, Tavner Delcamp, Michael T. DeYarman, James H. FreisheimCite this: Biochemistry 1992, 31, 1, 218–229Publication Date (Print):January 14, 1992Publication History Published online1 May 2002Published inissue 14 January...
A water-soluble, highly polar, heat-stable, small molecule has been isolated from cell-free extracts of the halotolerant green alga Dunaliella salina. This compound, soluble inhibitory factor (SIF), when added to in vivo light-activated, thylakoid membrane-bound preparations D. salina coupling 1 (CF1), causes a rapid inactivation ATPase activity. SIF must be its oxidized form inactivate CF1 and probably functions by oxidizing reduced light-activated enzyme. purified homogeneity characterized...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSecondary structure and topology of interleukin-1 receptor antagonist protein determined by heteronuclear three-dimensional NMR spectroscopyBrian J. Stockman, Terrence A. Scahill, Melinda Roy, Eldon L. Ulrich, Nancy Strakalaitis, David P. Brunner, Anthony W. Yem, Martin R. Deibel, Jr.Cite this: Biochemistry 1992, 31, 23, 5237–5245Publication Date (Print):July 16, 1992Publication History Published online1 May 2002Published inissue 16 July...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructural changes caused by site-directed mutagenesis of tyrosine-98 in Desulfovibrio vulgaris flavodoxin delineated 1H and 15N NMR spectroscopy: implications for redox potential modulationBrian J. Stockman, Thomas E. Richardson, Richard P. SwensonCite this: Biochemistry 1994, 33, 51, 15298–15308Publication Date (Print):December 1, 1994Publication History Published online1 May 2002Published inissue 1 December...