A5046299763- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Advanced Fluorescence Microscopy Techniques
- Advanced biosensing and bioanalysis techniques
- Neural dynamics and brain function
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
- Epigenetics and DNA Methylation
- Connexins and lens biology
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Nicotinic Acetylcholine Receptors Study
- Molecular Sensors and Ion Detection
- Gene Regulatory Network Analysis
- Microtubule and mitosis dynamics
- Medical Imaging Techniques and Applications
- Advanced MRI Techniques and Applications
- Bacterial Genetics and Biotechnology
- Forensic Fingerprint Detection Methods
- Heat shock proteins research
- Ion channel regulation and function
- Nuclear Physics and Applications
- bioluminescence and chemiluminescence research
Institute of Cancer Research
2022-2024
St George's, University of London
2017-2023
Significance Excitatory synapses convert presynaptic action potentials into chemical signals that are sensed by postsynaptic glutamate receptors. To eavesdrop on synaptic transmission, genetically encoded fluorescent sensors for have been developed. However, even the best available lag behind very fast dynamics in cleft. Here, we report development of an ultrafast sensor, iGlu u , which allowed us to image clearance and depression during 100-Hz spike trains. We found only boutons showing...
Measuring the dynamics with which regulatory complexes assemble and disassemble is a crucial barrier to our understanding of how cell cycle controlled that until now has been difficult address. This considerable gap in due difficulty reconciling biochemical assays single cell-based techniques, but recent advances microscopy gene editing techniques enable measurement kinetics protein–protein interaction living cells. Here, we apply fluorescence correlation spectroscopy cross-correlation study...
The proper control of mitosis depends on the ubiquitin-mediated degradation right mitotic regulator at time. This is effected by Anaphase Promoting Complex/Cyclosome (APC/C) ubiquitin ligase that regulated Spindle Assembly Checkpoint (SAC). SAC prevents APC/C from recognising Cyclin B1, essential anaphase and cytokinesis inhibitor, until all chromosomes are attached to spindle. Once attached, B1 rapidly degraded enable chromosome segregation cytokinesis. We have a good understanding how...
Ca2+/calmodulin (Ca2+/CaM) interaction with connexins (Cx) is well-established; however, the mechanistic basis of regulation gap junction function by Ca2+/CaM not fully understood. predicted to bind a domain in C-terminal portion intracellular loop (CL2) vast majority Cx isoforms and for number Cx-s this prediction has proved correct. In study, we investigate characterise both apo-CaM binding selected representatives each α, β γ connexin family develop better understanding CaM effects on...
ABSTRACT Glutamatergic synapses display a rich repertoire of plasticity mechanisms on many different time scales, involving dynamic changes in the efficacy transmitter release as well number and function postsynaptic glutamate receptors. The genetically encoded sensor iGluSnFR enables visualization from presynaptic terminals at frequencies up to ∼10 Hz. However, resolve dynamics during high frequency bursts, faster indicators are required. Here we report development fast (iGlu f ) ultrafast...
ABSTRACT Protein-based fluorescent glutamate sensors have the potential for real-time monitoring of synaptic and cellular concentration changes, however even fastest currently available sensors’ response times 2-3 ms are too slow accurate reporting post-synaptic AMPA receptor function in physiological conditions. We developed probes based on bacterial periplasmic glutamate/aspartate binding protein with either an endogenously or a synthetic fluorophore as indicator binding: affinity variants...
Abstract Measuring the dynamics with which regulatory complexes of cell cycle machinery assemble and disassemble is a crucial barrier to our understanding that until now has been difficult address. This considerable gap in due difficulty reconciling biochemical assays single cell-based techniques, but recent advances microscopy gene editing techniques enable measurement protein-protein interaction kinetics living cells. Here, we apply Fluorescence Correlation Spectroscopy (FCS)...
ABSTRACT The proper control of mitosis depends on the ubiquitin-mediated degradation right mitotic regulator at time. This is under anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase that regulated by Spindle Assembly Checkpoint (SAC). prevents APC/C from recognizing Cyclin B1, essential and cytokinesis inhibitor, until all chromosomes are attached to spindle. Once attached, B1 rapidly degraded enable chromosome segregation cytokinesis. We have a good understanding how SAC...