A5057812053- Diet and metabolism studies
- Pancreatic function and diabetes
- Gut microbiota and health
- Diet, Metabolism, and Disease
- Diabetes Treatment and Management
- Tryptophan and brain disorders
- Maternal Mental Health During Pregnancy and Postpartum
- Gestational Diabetes Research and Management
- Regulation of Appetite and Obesity
- Metabolism, Diabetes, and Cancer
- Biochemical Analysis and Sensing Techniques
- Genetic Syndromes and Imprinting
- Dietary Effects on Health
- Adipose Tissue and Metabolism
- Diabetes and associated disorders
- Birth, Development, and Health
University of Illinois Chicago
2019-2023
University of Illinois Urbana-Champaign
2021-2022
Illinois College
2019-2021
Background & aimsNormal gestation involves a reprogramming of the maternal gut microbiome (GM) that contributes to metabolic changes by unclear mechanisms. This study aimed understand mechanistic underpinnings GM—maternal metabolism interaction.MethodsThe GM and plasma metabolome CD1, NIH-Swiss, C57 mice were analyzed with use 16S rRNA sequencing untargeted liquid chromatography–mass spectrometry throughout gestation. Pharmacologic genetic knockout mouse models used identify role indoleamine...
Short-chain fatty acids (SCFAs) are key nutrients that play a diverse set of roles in physiological function, including regulating metabolic homeostasis. Generated through the fermentation dietary fibers distal colon by gut microbiome, SCFAs and their effects partially mediated cognate receptors, free acid receptor 2 (FFA2). FFA2 is highly expressed intestinal epithelial cells, where its putative functions controversial, with numerous vivo studies relying on global knockout mouse models to...
Here, we generated a novel intestine-specific knockout mouse model for FFA3 (Vil-FFA3) and performed comprehensive metabolic characterization of mice in response to an obesogenic challenge. We found that Vil-FFA3 fed with Western diet were largely protected from obesity, exhibiting significantly lower levels fat mass, intestinal inflammation, altered expression incretin hormones. Results support important role contributing metabolism the development diet-induced obesity.
Olfactory receptor OLFR78 is a G protein-coupled (GPCR) which activated by short chain fatty acids (SCFAs), especially propionate. expressed in islets as well intestinal epithelial cells. Based on these properties, we hypothesized its role the regulation of metabolic hormone secretion. Our emerging data shows that signaling regulates GLP-1 secretion from murine enteroendocrine cells vitro and ex vivo. We demonstrate knockdown Olfr78 through siRNA cell line STC-1 results significant reduction...
Abstract Free fatty acid receptor 2 and free 3 (FFA2/3) are two highly similar G protein-coupled receptors belonging to the family. Their ligands short-chain acids (SCFAs), which key nutrients that play a diverse role in physiological function, including regulation of metabolic homeostasis glycemic control. FFA2/3 broadly expressed multitude tissues intestine, pancreas, adipose central nervous system, where they contribute via summation tissue-specific effects. Consequently, have been...
Abstract Free fatty acid receptor 3 (FFA3) is a recently-deorphaned G protein-coupled belonging to the free family. Its ligands are short-chain acids (SCFAs), which key nutrients that play diverse role in physiological function, including regulation of metabolic homeostasis and glycemic control. FFA3 broadly expressed multitude tissues intestine, pancreas, central nervous system, thought contribute via summation its tissue-specific effects. Consequently, has been identified as potential drug...
Abstract Background and aims Normal gestation involves reprogramming of maternal gut microbiome (GM) that may contribute to metabolic changes by unclear mechanisms. This study aimed understand the mechanistic underpinnings GM – metabolism interaction. Methods The plasma metabolome CD1, NIH-Swiss C57BL/6J mice were analyzed using 16S rRNA sequencing untargeted LC-MS throughout postpartum. Pharmacologic genetic knockout mouse models used identify role indoleamine 2,3-dioxygenase (IDO1) in...