A5079813804- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
- Wastewater Treatment and Reuse
- Fecal contamination and water quality
- Pharmaceutical and Antibiotic Environmental Impacts
- Food Safety and Hygiene
- Soil and Unsaturated Flow
- Microbial Inactivation Methods
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Water Treatment and Disinfection
- Hydrology and Watershed Management Studies
- Essential Oils and Antimicrobial Activity
- Agriculture Sustainability and Environmental Impact
- Composting and Vermicomposting Techniques
- Environmental remediation with nanomaterials
- Vibrio bacteria research studies
- Groundwater flow and contamination studies
- Plant Water Relations and Carbon Dynamics
- Plant Virus Research Studies
- Soil erosion and sediment transport
- Urban Stormwater Management Solutions
- Water-Energy-Food Nexus Studies
- Bacterial biofilms and quorum sensing
United States Department of Agriculture
2014-2025
Agricultural Research Service
2016-2025
Beltsville Agricultural Research Center
2016-2025
Agricultural Research Service - Northeast Area
2017-2024
Patanjali Research Foundation
2024
Graphic Era University
2023
Microsoft Research (India)
2023
Delhi Technological University
2023
Indian Institute of Information Technology Guwahati
2020-2022
Netaji Subhas University of Technology
2022
To determine the effectiveness of an alkaline cleaner used in food-processing plants and a lytic bacteriophage specific for Escherichia coli O157:H7 killing wild type rpoS-deficient cells pathogen biofilm.Wild were attached to stainless steel coupons (c. 7-8 log CFU per coupon) on which biofilms developed during incubation at 22 degrees C 96 h M9 minimal salts media (MSM) with one transfer fresh medium. Coupons treated 100 25% working concentrations commercial (pH 11.9, microg ml(-1) free...
Scaling theory, based on the similar media concept, provides a basis for representing soil spatial variability in terms of single stochastic variable, scaling factor α, which is related to microscopic characteristic length soil. Based measured properties α (log normally distributed with an arithmetic mean 1.0 and coefficient variation 0.6), effects water balance components grassland watershed near Chickasha, Oklahoma, were studied by using simulation model. Monthly simulations...
Journal Article Effect of curli expression and hydrophobicity Escherichia coli O157:H7 on attachment to fresh produce surfaces Get access J. Patel, Patel USDA, Agricultural Research Service, Environmental Microbial Food Safety Laboratory, Beltsville, MA, USA Jitendra 10300 Baltimore Avenue, BARC‐East, Bldg. 201, MA 20705‐2350, USA. E‐mail: jitu.patel@ars.usda.gov Search for other works by this author on: Oxford Academic Google Scholar M. Sharma, Sharma S. Ravishakar Department Veterinary...
The effect of a bacteriophage cocktail (EcoShield™) that is specific against Escherichia coli O157:H7 was evaluated nalidixic acid-resistant enterohemorrhagic E. RM4407 (EHEC) strain on leafy greens stored under either (1) ambient air or (2) modified atmosphere (MA; 5% O2/35% CO2/60% N2). Pieces (~2 × 2 cm2) (lettuce and spinach) inoculated with 4.5 log CFU/cm2 EHEC were sprayed EcoShield™ (6.5 PFU/cm2). Samples at 4 10°C for up to 15 d. On spinach, the level declined by 2.38 2.49 10°C,...
The role of lytic bacteriophages in preventing cross contamination produce has not been evaluated. A cocktail three phages specific for E. coli O157:H7 (EcoShield™) or a control (phosphate buffered saline, PBS) was applied to lettuce by either; (1) immersion 500 ml EcoShield™ 8.3 log PFU/ml 9.8 up 2 min before inoculation with O157:H7; (2) spray-application (9.3 PFU/ml) after (4.10 CFU/cm2) following exposure 50 μg/ml chlorine 30 sec. After studies, spot-inoculated (2.38 CFU/cm2)....
Untreated biological soil amendments of animal origin (BSAAO), such as manure, are commonly used to fertilize soils for growing fruit and vegetable crops can contain enteric bacterial foodborne pathogens. Little is known about the comparative longitudinal survival pathogens in agricultural fields containing different types BSAAO, field data may be useful determine intervals between manure application harvest produce intended human consumption minimize illness. This study generated 324...
Irrigation water contaminated with Salmonella enterica and Listeria monocytogenes may provide a route of contamination raw or minimally processed fruits vegetables. While previous work has surveyed specific singular types agricultural irrigation for bacterial pathogens, few studies have simultaneously different sources repeatedly over an extended period time. This study quantified S. L. levels (MPN/L) at 6 sites, including river waters: tidal freshwater (MA04, n = 34), non-tidal river,...
Antimicrobial resistance (AMR) is a world-wide public health threat that projected to lead 10 million annual deaths globally by 2050. The AMR issue has led the development of action plans combat AMR, including improved antimicrobial stewardship, new antimicrobials, and advanced monitoring. National Resistance Monitoring System (NARMS) United States (U.S) Food Drug Administration along with U.S. Centers for Disease Control Department Agriculture monitored resistant bacteria in retail meats,...
The environment plays a crucial role in the spread of antibiotic resistance, however environmental surveillance efforts lag far behind clinical surveillance. Natural and human-impacted soil water are considered major sources resistance medical veterinary diagnostics, lack agricultural data hinders to assess management impacts or estimate risk. Here we introduce two agriculturally sourced, fully characterized, genetically sequenced control strains for use extended-spectrum β-lactamase...
A study was performed to determine D values of acid-adapted and unadapted cells Salmonella, Escherichia coli O157:H7, Listeria monocytogenes in cantaloupe juice watermelon juice.Salmonella enterica serotype Poona, S. Saphra, two strains E. L. were grown tryptic soy broth (TSB) TSB supplemented with 1% glucose for 24 h at 37 degrees C. Decimal reduction times (D values) suspended unpasteurized determined. Acid-adapted Salmonella but not monocytogenes, had increased thermal tolerance compared...
Outbreaks associated with leafy greens have focused attention on the transfer of human pathogens to these commodities during harvest commercial equipment. Attachment Escherichia coli O157:H7 new or rusty spinach harvester blades immersed in extract 10% tryptic soy broth (TSB) was investigated. Bacteriophages specific for E. were evaluated kill cells attached blade. A cocktail five nalidixic acid-resistant isolates transferred 25 mL TSB. piece sterilized blade (2 × 1") placed above TSB and...
Biological soil amendments (BSAs) are essential agricultural inputs that provide critical nutrients in organic leafy green production. Heat-treated poultry pellets (HTPP) and seabird guano (SBG), which have been treated to reduce microbial pathogen loads, gaining popularity among growers. Using these BSAs the process of side-dressing, providing crops while growing, may opportunities for externally introduced bacterial pathogens survive soil. In this study, Romaine lettuce was grown soils a...
Antimicrobial resistance (AMR) in irrigation waters is a major worldwide health issue. Crops irrigated with containing antibiotic resistant bacteria (ARB) or related genes (ARG) can serve as vector for AMR throughout food supply systems. The current extent of poorly understood and even less so small lentic such farm ponds. objectives this work were to characterize the variability ARG concentrations an actively used pond determine if stable spatial patterns concentration data exist which be...
Abstract Quantification of Escherichia coli in water is commonly used to understand a surface source's suitability for produce irrigation. Location, season, and physicochemical quality impact the levels E. irrigation ponds. Water samples were collected periodically at three ponds Southeast Georgia along sampling grid from July 2021 through September 2023 quantified with simultaneous collection relevant parameters. Mean relative differences (MRDs) calculated each point determine across...