A5059396044- Cellular transport and secretion
- RNA Research and Splicing
- Pancreatic function and diabetes
- RNA modifications and cancer
- Retinal Development and Disorders
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Calcium signaling and nucleotide metabolism
- Evolution and Genetic Dynamics
- Viral Infections and Immunology Research
- Lipid Membrane Structure and Behavior
- Plant Virus Research Studies
- Malaria Research and Control
- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
- Mosquito-borne diseases and control
- Transgenic Plants and Applications
- Lipid metabolism and biosynthesis
- Insect symbiosis and bacterial influences
- RNA Interference and Gene Delivery
- Lysosomal Storage Disorders Research
- Protein Kinase Regulation and GTPase Signaling
- Phagocytosis and Immune Regulation
University of California, San Francisco
2018-2024
Weizmann Institute of Science
2009-2013
Technion – Israel Institute of Technology
2005
The conserved oligomeric Golgi (COG) complex has been implicated in the regulation of endosome to trans-Golgi network (TGN) retrograde trafficking both yeast and mammals. However, exact mechanisms by which it regulates this transport route remain largely unknown. In paper, we show that COG interacts directly with target membrane SNARE (t-SNARE) Syntaxin 6 via Cog6 subunit. Cog6-depleted cells, steady-state level was markedly reduced, concomitantly, endosome-to-TGN traffic significantly...
Multisubunit tethering complexes (MTCs) positively regulate vesicular fusion by as yet unclear mechanism. In this study we provide evidence that the MTC COG enhances assembly of fusogenic Golgi SNARE and concomitantly prevents nonfusogenic tSNARE interactions. This capability is possibly mediated multiple direct interactions subunits specific SNAREs SM (Sec1/Munc18) proteins. By using a systematic co-immunoprecipitation analysis, identified seven new between components machinery in mammalian...
Multiple mutations in different subunits of the tethering complex Conserved Oligomeric Golgi ( COG ) have been identified as a cause for Congenital Disorders Glycosylation CDG humans. Yet, mechanisms by which induce pleiotropic defects not fully defined. By detailed analysis Cog8 deficiency either HeLa cells or ‐derived fibroblasts, we show that is required assembly both and Stx5‐ GS28 ‐Ykt6‐ GS15 Stx6‐Stx16‐Vti1a‐ VAMP4 SNARE complexes. The these complexes also impaired derived from...
Translin is a human octameric protein that specifically binds the single-stranded microsatellite repeats d(GT)n and corresponding transcripts (GU)n. It also binds, with lesser affinities, other G-rich DNA RNA sequences. TRAX bears homology to interacts it. have been proposed be involved in recombination, chromosomal translocation mRNA transport translation. Both proteins are highly conserved eukaryotes, including fission yeast Schizosaccharomyces pombe, which amenable genetic analysis. Here,...
Translin is a human single-stranded DNA and RNA binding protein that has been highly conserved in eukaryotic evolution. It consists of eight subunits having helical secondary structure assemble into ring. The the are bound inside Recently, some us demonstrated translin specifically binds microsatellite repeats, d(GT)(n), telomeric d(TTAGGG)(n), Tetrahymena d(GGGGTT)(n). These data suggested might be involved recombination at d(GT)(n).d(AC)(n) microsatellites telomere metabolism. Other...
Positive-stranded RNA viruses extensively remodel host cell architecture to enable viral replication. We examined the poorly understood formation of specialized membrane compartments which are critical sites for synthesis genome. show that replication (RCs) enteroviruses created through novel contact recruit lipid droplets (LDs) RCs. Highly conserved proteins physically tether RCs LDs and interact with lipolysis machinery mediate transfer fatty acids from LDs, thereby providing lipids...
Exploring the intricacies of host-pathogen communication is vital in order to understand why some microbes persist within a host, while others are effectively cleared. As such, an urgent area research underpinning human health and disease, especially given emerging concerns antibiotic resistance. Understanding pathways requires intricate knowledge fields both microbiology immunology. Living world specialists, where scientists sometimes invest their whole career studying one organism,...