A5001662652- Central Venous Catheters and Hemodialysis
- Vascular Malformations Diagnosis and Treatment
- Aortic aneurysm repair treatments
- Vascular anomalies and interventions
- Atherosclerosis and Cardiovascular Diseases
- Microbial metabolism and enzyme function
- Reproductive Physiology in Livestock
- Single-cell and spatial transcriptomics
- Peripheral Artery Disease Management
- Renal and Vascular Pathologies
- Vascular Procedures and Complications
- Vascular Anomalies and Treatments
- Skin Protection and Aging
- Infectious Aortic and Vascular Conditions
- Nitric Oxide and Endothelin Effects
- Phytochemicals and Antioxidant Activities
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Chromatin Dynamics
- Ferroptosis and cancer prognosis
- Chronic Kidney Disease and Diabetes
- Receptor Mechanisms and Signaling
- Pomegranate: compositions and health benefits
- Connective tissue disorders research
- Cancer-related molecular mechanisms research
University of Miami
2019-2024
Langston University
2019-2021
Instituto Tecnológico de Costa Rica
2013-2020
American Institute for Economic Research
2019-2020
Arteries and veins develop different types of occlusive diseases respond differently to injury. The biological reasons for this discrepancy are not well understood, which is a limiting factor the development vein-targeted therapies. This study contrasts human peripheral arteries at single-cell level, with focus on cell populations remodeling potential. Upper arm (brachial) (basilic/cephalic) from 30 organ donors were compared using combination bulk RNA sequencing, proteomics, flow cytometry,...
The venous system has been historically understudied despite its critical roles in blood distribution, heart function, and systemic immunity. This study dissects the microanatomy of upper arm veins at single cell level, how it relates to wall structure, remodeling processes, inflammatory responses injury. We applied single-cell RNA sequencing 4 non-diseased human (3 basilic, 1 cephalic) obtained from organ donors, followed by bioinformatic histological analyses. Unsupervised clustering...
The biological mechanisms underlying arteriovenous fistula (AVF) maturation in hemodialysis patients remain poorly understood despite decades of research. To address this gap, we investigated the cellular changes venous wall after creation histological biopsies longitudinal veins and AVF samples (N=23 patients). Using single-cell RNA sequencing 70,281 cells from pre-access veins, mature, failed AVFs (N=20 patients), created a complementary transcriptomic atlas human vein before anastomosis....
The molecular transformation of the human preaccess vein after arteriovenous fistula (AVF) creation is poorly understood. This limits our ability to design efficacious therapies improve maturation outcomes.
Solar ultraviolet (UV) radiation, particularly its UVB (280-320 nm) spectrum, is the primary environmental stimulus leading to skin carcinogenesis. Several botanical species with antioxidant properties have shown photochemopreventive effects against damage. Costa Rica's tropical highland blackberry (Rubus adenotrichos) contains important levels of phenolic compounds, mainly ellagitannins and anthocyanins, strong properties. In this study, we examined effect R. adenotrichos juice (BBJ) on...
Background Arteriovenous fistula (AVF) postoperative stenosis is a persistent healthcare problem for hemodialysis patients. We have previously demonstrated that fibrotic remodeling contributes to AVF non-maturation and lysyl oxidase (LOX) upregulated in failed AVFs compared matured. Herein, we developed nanofiber scaffold the periadventitial delivery of β-aminopropionitrile (BAPN) determine whether unidirectional LOX inhibition suitable strategy promote adaptive rat model remodeling. Methods...
Background The arteriovenous fistula (AVF) is the preferred hemodialysis access for patients with ESKD. Yet, establishment of a functional AVF presents challenge, even most experienced surgeons, because postoperative stenosis frequently occludes AVF. Stenosis results from loss compliance in fibrotic areas fistula, which turns intimal hyperplasia into an occlusive feature. Fibrotic remodeling depends on deposition and crosslinking collagen by lysyl oxidase (LOX), enzyme that catalyzes...
Background Systemic cytokines are elevated in patients with chronic kidney disease (CKD) and on hemodialysis compared the general population. However, whether cytokine levels interfere vascular remodeling, increasing risk of arteriovenous fistula (AVF) failure, remains unknown. Methods This is a case-control study 64 who underwent surgery for AVF creation (32 maturation failure 32 matching controls successful maturation). A total 74 cytokines, including chemokines, interferons, interleukins,...
Aims: Arteries and veins respond differently to injury, with significantly higher rates of postsurgical failure in the latter. The biological reasons for this discrepancy are not well understood, which is a limiting factor development vein-targeted therapies. This study contrasts peripheral arteries at single-cell level, focus on cell populations remodeling potential. Methods Results: Upper arm (brachial) (basilic/cephalic) from 27 organ donors were compared using combination bulk RNA...
Objective: Collagen VIII is a basement collagen upregulated in processes of vascular remodeling. We recently demonstrated the association increased COL8A1 expression and arteriovenous fistula failure human cohort. This study analyzed effect loss murine model. Methods: Arteriovenous fistulas were created between external jugular vein common carotid artery 5/6 nephrectomized Col8a1/a2 null mice wild type controls (n=8-9 per group). Intimal hyperplasia was quantified Masson’s trichrome stained...
Abstract The objective was to determine if effects of level salinity in drinking water for yearling meat goat wethers consuming low-protein roughage are influenced by protein supplementation and breed. Eighteen Boer (initial BW 37.9±1.12 kg 1.15±0.009 yr) 18 Spanish goats (33.5±0.66 1.16±0.008 consumed wheat straw ad libitum 10 wk. Soybean meal supplemented at 0.13–0.14 or 0.27–0.28% (DM; Low High, respectively) fresh alone (240 mg/kg total dissolved salts) with NaCl added 5,000 10,000 mg/L...
Methods: Twenty-six hundred cells from 4 human peripheral veins and 2 arteries were droplet-based single cell sequenced using the 10X Chromium protocol . Paired bulk RNA-seq seven obtained same patient immunofluorescence validated findings. Results: Arteries richer in smooth muscle (39.1 vs. 13.8%) while vein contained abundant immune (14.5 39.8%). Both vessels had fibroblasts, (28.1 34.5%), endothelial Cells (16.5 11.1 %). Concordantly, contractility ECM-related pathways significantly...
Objective: A comprehensive picture of the cellular events dictating transformation vein after arteriovenous fistula (AVF) creation has remained elusive so far. This study generated first granular atlas pre-access veins and AVF from patients with end-stage kidney disease (ESKD). Methods: Veins (n=5) (n=7) were obtained a total 12 ESKD undergoing in two stages. 41,000 enzymatically dissociated cells single cell sequenced using 10x Chromium system. Monocle3 inferred trajectory leading to...