A5010612069- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Gene Regulatory Network Analysis
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Physiological and biochemical adaptations
- Escherichia coli research studies
- Insect Resistance and Genetics
- Microbial Metabolic Engineering and Bioproduction
- Invertebrate Immune Response Mechanisms
- Neurobiology and Insect Physiology Research
- Chemical Synthesis and Analysis
- Transgenic Plants and Applications
- Viral Infections and Immunology Research
Eligo Bioscience (France)
2022-2025
Imperial College London
2016-2020
Fraunhofer Institute for Cell Therapy and Immunology
2013-2014
Fraunhofer Institute for Biomedical Engineering
2013
Fraunhofer Society
2012-2013
Abstract Microbiome research is now demonstrating a growing number of bacterial strains and genes that affect our health 1 . Although CRISPR-derived tools have shown great success in editing disease-driving human cells 2 , we currently lack the to achieve comparable for targets situ. Here engineer phage-derived particle deliver base editor modify Escherichia coli colonizing mouse gut. Editing β-lactamase gene model E. strain resulted median efficiency 93% target population with single dose....
ABSTRACT Protein expression systems are widely used in biotechnology and medicine for the efficient economic production of therapeutic proteins. Today, cultivated Chinese hamster ovary (CHO) cells market dominating mammalian cell‐line complex Despite this outstanding potential CHO cells, no high‐yield cell‐free system based on translationally active lysates from these has been reported so far. To date, cell extracts have only as a foundational research tool understanding mRNA translation...
Internal ribosome entry site (IRES) elements found in the 5′ untranslated region of mRNAs enable translation initiation a cap-independent manner, thereby representing an alternative to cap-dependent cell-free protein expression systems. However, IRES function is largely species-dependent so their utility systems from different species rather limited. A promising approach overcome these limitations would be use IRESs that are able recruit components apparatus diverse origins. Here, we present...
Abstract Synthetic biology has seen an explosive growth in the capability of engineering artificial gene circuits from transcription factors (TFs), particularly bacteria. However, most networks still employ same core set TFs (for example LacI, TetR and cI). The mostly function via repression it is difficult to integrate multiple inputs promoter logic. Here we present our knowledge first dual activator-repressor switches for orthogonal logic gates, based on bacteriophage λ cI variants...
Abstract Escherichia coli is a ubiquitous gut commensal but also an opportunistic pathogen responsible for severe intestinal and extra-intestinal infections. Shiga toxin-producing E. (STEC) pose significant public health threat, particularly in children, where infections can lead to bloody diarrhea progress hemolytic uremic syndrome (HUS), life-threatening condition with long-term complications. Antibiotics are contraindicated STEC due their potential induce prophages carrying toxin ( stx)...
The smallest gene activator-repressor with polymerase recruitment is engineered using a 63–amino acid peptide.
Sophisticated cell-free protein synthesis (CFPS) systems have been developed as an alternative to recombinant expression in cultured cells. In this review, we present advances the field of mammalian-based CFPS by highlighting recently established derived from mouse fibroblasts, HeLa, hybridoma, CHO and K562 We further highlight ongoing challenges CFPS, such optimization already platforms development novel order increase yields reduce manufacturing costs while facilitating a huge number...
Abstract Microbiome research is revealing a growing number of bacterial genes that impact our health. While CRISPR-derived tools have shown great success in editing disease-driving human cells, we currently lack the to achieve comparable for targets. Here engineer phage-derived particle deliver base editor and modify E. coli colonizing mouse gut. This was achieved using non-replicative DNA payload, preventing maintenance dissemination while allowing an efficiency up 99.7% target population....
Transcription factors control gene expression in all life. This raises the question of what is smallest protein that can support such activity. In nature, Cro from bacteriophage λ known repressor (66 amino acids; a.a.) but activators are typically much larger (e.g. cI, 237 a.a.). Indeed, previous efforts to engineer a minimal activator resulted no activity vivo . this study, we show directed evolution results new activator-repressor functions as efficiently To achieve this, develop...