A5080168257- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- Cellular transport and secretion
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Glycosylation and Glycoproteins Research
- Plant Gene Expression Analysis
- Plant pathogens and resistance mechanisms
- Plant Parasitism and Resistance
- Plant Reproductive Biology
- Fibroblast Growth Factor Research
- Biotin and Related Studies
- Fern and Epiphyte Biology
- Metabolomics and Mass Spectrometry Studies
- RNA modifications and cancer
- Child Abuse and Trauma
- RNA and protein synthesis mechanisms
- Child and Adolescent Psychosocial and Emotional Development
- Biosensors and Analytical Detection
- Plant Pathogenic Bacteria Studies
- Plant Molecular Biology Research
- Lipid Membrane Structure and Behavior
- Phagocytosis and Immune Regulation
- Trypanosoma species research and implications
University of Washington
2023-2025
University of Florida
2018-2024
Guangdong University of Foreign Studies
2020
Sample multiplexed quantitative proteomics assays have proved to be a highly versatile means assay molecular phenotypes. Yet, stochastic precursor selection and coisolation can dramatically reduce the efficiency of data acquisition accuracy. To address this, intelligent (IDA) strategies recently been developed improve instrument accuracy for both discovery targeted methods. Toward this end, we sought develop implement new real-time spectral library searching (RTLS) workflow that could enable...
Arabidopsis MAP kinase 4 (MPK4) has been proposed to be a negative player in plant immunity, and it is also activated by pathogen-associated molecular patterns (PAMPs), such as flg22. The mechanisms which MPK4 regulates defense remain elusive. In this study, we investigated against bacterial pathogen Pseudomonas syringae pv tomato (Pst) DC3000 when Brassica napus (BnMPK4) overexpressed. We showed an increase resistance suppression of jasmonic acid (JA) signaling the BnMPK4 overexpressing...
Glycosylphosphatidylinositol (GPI) anchorage of cell surface proteins to the membrane is biologically important and ubiquitous in eukaryotes. However, GPIs do not contain long enough lipids span entire bilayer. To transduce binding signals, must interact with other components, but such interactions are difficult define. Here, a new method was developed explore GPI-interacting live bifunctional analogue glucosaminylphosphatidylinositol motif conserved all as probe. This probe contained...
Summary Epigenetic inhibitors exhibit powerful antiproliferative and anticancer activities. However, cellular responses to small-molecule epigenetic inhibition are heterogenous dependent on factors such as the genetic background, metabolic state, on-/off-target engagement of individual compounds. The molecular study extent this heterogeneity often measures changes in a single cell line or using small number To more comprehensively profile effects perturbations their influence these...
Mitogen-activated protein kinase 4 (MPK4) was first identified as a negative regulator of systemic acquired resistance. It is also an important involved in many other biological processes plants, including cytokinesis, reproduction, and photosynthesis. Arabidopsis thaliana mpk4 mutant dwarf sterile. Previous omics studies genomics, transcriptomics, proteomics have revealed new functions MPK4 different processes. However, due to challenges metabolomics, no study has touched upon the...
Mitogen-activated protein kinase (MPK) cascades play important roles in plant development, immune signaling and stress responses. MPK4 was initially identified as a negative regulator systemic acquired resistance (SAR) because the levels of salicylic acid (SA) reactive oxygen species (ROS) were higher Arabidopsis mpk4 mutant. is highly expressed guard cells, specialized epidermal cells forming stomatal pores on leaf surface that function at frontline bacterial pathogen invasion. In addition...
Identification of kinase substrates is a prerequisite for elucidating the mechanism by which transduces internal or external stimuli to cellular responses. Conventional methods profile this type protein-protein interaction typically deal with one kinase-substrate pair at time. Mass spectrometry-based proteomics, on other hand, can determine putative pairs large-scale in an unbiased manner. In study, we identified interacting partners SNF1-related protein 2.4 (SnRK2.4) via immunoprecipitation...
Abstract Sample multiplexed quantitative proteomics has proved to be a highly versatile means assay molecular phenotypes. Yet, stochastic precursor selection and co-isolation can dramatically reduce the efficiency of data acquisition accuracy. To address this, intelligent (IDA) strategies have recently been developed improve instrument accuracy for both discovery targeted methods. Towards this end, we sought develop implement new real-time library searching (RTLS) workflow that could enable...
The primary goal of this study was to determine the effect sonication on stomatal movement. A minor best time interval at which is most effective removing mesophyll cells and enriching guard cells. For study, abaxial leaf peels Arabidopsis thaliana were sonicated for 1, 3, 5, 7-minute intervals a set amplitude analyze removal To juxtapose leaves cell enrichment, microscopic images taken prior after sonication. Furthermore, establish that stomata are alive, neutral red staining used in...