A5029550427- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Influenza Virus Research Studies
- Advanced Biosensing Techniques and Applications
- Respiratory viral infections research
- Advanced Nanomaterials in Catalysis
- Viral gastroenteritis research and epidemiology
- Gold and Silver Nanoparticles Synthesis and Applications
- Monoclonal and Polyclonal Antibodies Research
- Optical Imaging and Spectroscopy Techniques
- Mycobacterium research and diagnosis
- Retinal Imaging and Analysis
- Tuberculosis Research and Epidemiology
- Viral Infections and Immunology Research
- Retinal and Optic Conditions
- Electrochemical sensors and biosensors
- Nanoplatforms for cancer theranostics
- Infectious Encephalopathies and Encephalitis
- Bacterial Infections and Vaccines
- Bacteriophages and microbial interactions
National Nanotechnology Center
2012-2024
National Science and Technology Development Agency
2012-2024
Pathumthani University
2016-2021
A novel sensitive SERS-lateral flow immunochromatographic integration system using Raman active molecule-coated gold nanostar as reporters for influenza virus detection is reported. Qualitative and quantitative SERS signal can be achieved.
Several existing molecular tests for multidrug-resistant tuberculosis (MDR-TB) are limited by complexity and cost, hindering their widespread application. The objective of this proof concept study was to develop a simple Nucleic Acid Lateral Flow (NALF) immunoassay as potential diagnostic alternative, complement conventional PCR, the rapid detection MDR-TB. NALF device designed using antibodies indirect labeled PCR amplification products. Multiplex optimized permit simultaneous drug...
Simple colorimetric system based on gold nanorods-graphene oxide self-assembly for heparin detection using a polycationic polymer as molecular probe is shown here.
Here, we report a simple colorimetric detection method for the determination of protamine based on self-assembly gold nanorods (AuNRs) graphene oxide (GO).
A simple fluorescence-based lateral flow test platform for rapid influenza B virus screening as a model target molecule was successfully developed. In this work, Cy5-loaded silica nanoparticles were directly conjugated to monoclonal antibodies, specific the nucleoprotein, via direct physisorption method and used detector probes. Using approach, signal response detection further determined using fluorescent intensity measurement portable reader, in combination with fluorescence imaging...
In this study, we investigated a feasibility of using carbon nanostrings as reporters in developing lateral flow immunochromatographic system for rapid influenza A antigen detection model. Carbon were successfully conjugated to monoclonal antibody specific the nucleoprotein, one most abundant protein, signal visualization. To demonstrate detection, was applied detect an allantoic fluid infected with pandemic 2009 H1N1 virus. It shown that able nucleoprotein complex biological such containing...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infections have affected more than 769 million individuals worldwide over the last few years. Although pandemic is transitioning into an endemic, COVID-19 outbreak still a global concern. A rapid screening platform needed for effective preventive and control measures. Herein, visual lateral flow SARS-CoV-2 nucleocapsid protein detection developed. Under optimal conditions, system demonstrated good sensitivity selectivity against...
A combined gold nanoparticle (GNP)-monoclonal antibody (MAb) conjugate was generated for a direct influenza antigen detection using lateral flow assay (LF) format. The combination of GNP-MAb targeting both nucleoprotein (NP) and matrix protein (M) allows the multiple proteins. LF system consisting showed an improved sensitivity, compared to containing single only one target protein, either NP or M. This illustrated by 2- 260-fold lower limit than LF. As such, could provide basis developing...
A visual colorimetric rapid screening system based on a lateral flow device for simultaneous detection and differentiation between influenza B nucleoprotein as model was developed. Monoclonal antibodies, specific either or nucleoproteins, were evaluated their reactivities used targeting ligands. With the best antibody pairs selected, exhibited good specificity to both viruses without cross reactivity other closely related respiratory viruses. Further semi-quantitative analysis using strip...