Early and accurate detection of cancer often requires time-consuming techniques expensive instrumentation. To address these limitations, we developed a colorimetric assay for the direct diseased cells. The uses aptamer-conjugated gold nanoparticles to combine selectivity affinity aptamers spectroscopic advantages allow sensitive Samples with target cells present exhibited distinct color change while nontarget samples did not elicit any in color. also showed excellent sensitivity both naked...

Safe and effective photothermal therapy depends on efficient delivery of heat for killing cells molecular specificity targeting cells. To address these requirements, we have designed an aptamer-based nanostructure which combines the high absorption efficiency Au-Ag nanorods with target aptamers, a combination resulting in development selective therapeutic agent targeted cancer cell destruction. Most nanomaterials, such as gold nanoshells or (NRs), require relatively power laser irradiation...

Aptamer-conjugated nanoparticles (ACNPs) have been used for a variety of applications, particularly dual magnetic extraction and fluorescent labeling. In this type assay, silica-coated fluorophore-doped silica are conjugated to highly selective aptamers detect extract targeted cells in matrixes. However, considerable improvements required order increase the selectivity sensitivity two-particle assay be useful clinical setting. To accomplish this, several parameters were investigated,...

Biocompatible magnetic nanosensors based on reversible self-assembly of dispersed nanoparticles into stable nanoassemblies have been used as effective relaxation switches (MRSw) for the detection molecular interactions. We report, first time, design MRSw aptamer-conjugated (ACMNPs). The ACMNPs capitalize ability aptamers to specifically bind target cancer cells, well large surface area MNPs accommodate multiple aptamer binding events. can detect few 10 cells in 250 μL sample. ACMNPs'...

The binding of proteins and small molecules by DNA is well established, but more recently, have been selected to catalyze biochemical reactions. These catalytic DNAs, or DNAzymes, can be activated metal ions. In this paper, we take advantage molecular engineering improve the properties DNAzymes designing a unimolecular probe for lead ion (Pb2+)-catalyzed reaction, achieving in turn, ability monitor single Pb2+ solution fluorescence microscopy. Specifically, applying design, leaving substrate...

Targeted chemotherapy and magnetic resonance imaging of cancer cells in vitro has been achieved using a smart multifunctional nanostructure (SMN) constructed from porous hollow magnetite nanoparticle (PHMNP), heterobifunctional PEG ligand, an aptamer. The PHMNPs were prepared through three-step reaction loaded with the anticancer drug doxorubicin while being functionalized ligands. Targeting aptamers then introduced by pores are stable at physiological pH, but they subject to acid etching....

An electrochemical biosensor employing a gold nanoparticles/graphene quantum dots/graphene oxide composite modified electrode is developed for the multiplex detection of miRNA breast cancer biomarkers.

An ultrasensitive electrochemical biosensor based on a gold nanoparticles/graphene/polypyrrole composite modified electrode and signal amplification strategy employing methylene blue is developed as potential tool for the detection of miRNA-21.

Magnetic relaxation switch (MRSw) detection is based on aggregate formation or dissociation when magnetic nanoparticles (MNPs) bind to target molecules. In the aggregated state, dephasing rate of nearby proton spins higher than in dispersed resulting a decrease spin–spin time, T2. this work, an MRSw-based nanosensor for lysozyme (Lys) protein was achieved using iron oxide conjugated with either Lys aptamer linker DNA, which can hybridize extended part form clusters. Upon addition Lys,...

A novel sensitive SERS-lateral flow immunochromatographic integration system using Raman active molecule-coated gold nanostar as reporters for influenza virus detection is reported. Qualitative and quantitative SERS signal can be achieved.

The quantification of alpha-fetoprotein (AFP) as a potential liver cancer biomarker which is generally found in ultratrace level significance biomedical diagnostics. Therefore, it challenging to find strategy fabricate highly sensitive electrochemical device towards AFP detection through electrode modification for signal generation and amplification. This work shows the construction simple, reliable, sensitive, label-free aptasensor based on polyethyleneimine-coated gold nanoparticles...

Here, we introduce an environmentally friendly approach to fabricate a simple and cost-effective plasmonic paper for detecting food additives using surface-enhanced Raman spectroscopy (SERS). The is fabricated by in situ growth of gold nanoparticles (AuNPs) on filter (FP). To facilitate this green fabrication process, applied double-layered coating biopolymers, chitosan (CS) alginate (ALG), onto the FP layer-by-layer (LbL) assembly through electrostatic interactions. Compared single-layer...

Lysozyme (Lys) plays crucial roles in the innate immune system, and detection of Lys urine serum has considerable clinical importance. Traditionally, presence been detected by immunoassays; however, these assays are limited availability commercial antibodies tedious protein modification prior sample purification. To address limitations, we report here design, synthesis, application a competition-mediated pyrene-switching aptasensor for selective buffer human serum. The strategy is based on...

Sometimes, smaller size is not always better, and looking for nanomaterials that offer better device performance requires consideration of their properties at the first stage. In this study, effects polyethylenimine-capped AuNPs (PEI-AuNPs) proteins on immunosensor performances, namely, sensitivity limit detection, are examined. The size-effect investigation PEI-AuNPs involves modification surface disposable screen-printed carbon electrodes to support primary antibodies ability load...

A robust water-in-oil microemulsion method of making water-dispersible ultra-small (<30 nm) size fluorescent chitosan nanoparticles is reported for the first time and specific targeting these FCNPs to human leukemia cellsviaaptamer recognition demonstrated.

A simple and cost-effective plasmonic paper as a SERS substrate based on combination of graphene oxide (GO) gold nanorods (AuNRs).