A5002102987- Salivary Gland Tumors Diagnosis and Treatment
- Tumors and Oncological Cases
- Oral and Maxillofacial Pathology
- Ear and Head Tumors
- Cancer Research and Treatments
- Head and Neck Surgical Oncology
- Neuroblastoma Research and Treatments
- Cancer-related gene regulation
- Head and Neck Cancer Studies
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Cancer-related molecular mechanisms research
- Cancer and Skin Lesions
- Aquaculture disease management and microbiota
- Chromatin Remodeling and Cancer
- Myasthenia Gravis and Thymoma
- Colorectal and Anal Carcinomas
- Pancreatic and Hepatic Oncology Research
- Salivary Gland Disorders and Functions
- Neuroendocrine Tumor Research Advances
- Sarcoma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Gastric Cancer Management and Outcomes
- Histone Deacetylase Inhibitors Research
The University of Texas MD Anderson Cancer Center
2015-2025
The Schwarzman Animal Medical Center
2025
Weatherford College
2023
Zero to Three
2018
South Texas Accelerated Research Therapeutics
2017
Hiroshima University
2003-2011
Baylor College of Medicine
2010-2011
Wellcome Sanger Institute
2011
The objectives of this study were to determine the incidence MYB-NFIB fusion in salivary adenoid cystic carcinoma (ACC), establish clinicopathologic significance fusion, and analyze expression MYB ACCs context fusion.We did an extensive analysis involving 123 cancers gland, including primary metastatic ACCs, non-ACC carcinomas. fusions identified by reverse transcriptase-PCR (RT-PCR) sequencing RT-PCR products, confirmed fluorescence situ hybridization. RNA was determined quantitative...
Adenoid cystic carcinoma (ACC) is a rare malignancy that can occur in multiple organ sites and primarily found the salivary gland. While identification of recurrent fusions MYB-NFIB genes have begun to shed light on molecular underpinnings, little else known about genetics this frequently fatal cancer. We undertaken exome sequencing series 24 ACC further delineate disease. identified mutated that, combined, implicate chromatin deregulation half cases. Further, mutations were cancer genes,...
Purpose Adenoid cystic carcinomas (ACCs) represent a heterogeneous group of chemotherapy refractory tumors, with subset demonstrating an aggressive phenotype. We investigated the molecular underpinnings this phenotype and assessed Notch1 pathway as potential therapeutic target. Methods genotyped 102 ACCs that had available pathologic clinical data. activation was by immunohistochemistry for intracellular domain. Luciferase reporter assays were used to confirm target gene expression in vitro....
Abstract Purpose: Adenoid cystic carcinoma (ACC) is an indolent salivary gland malignancy, characterized by t(6;9) translocations and MYB–NFIB gene fusions in approximately 50% of the tumors. The genetic alterations underlying t(6;9)-negative t(6;9)-positive/MYB–NFIB fusion–negative ACC remain unknown. To uncover lacking canonical translocation fusion transcript identify new abnormalities positive Experimental Design: We performed whole-genome sequencing 21 ACCs conducted targeted molecular...
Abstract Gastric carcinoma (GC) is one of the most common malignancies worldwide. To better understand genetic basis this disease, we performed serial analysis gene expression (SAGE) on four primary GC samples and associated lymph node metastasis. We obtained a total 137,706 expressed tags (Gene Expression Omnibus accession number GSE 545, SAGE Hiroshima gastric cancer tissue), including 38,903 that were unique. Comparing from our libraries containing different stages histologies, found...
Gastric cancer (GC) is one of the most common malignancies worldwide. Genes whose expression down-regulated in GC may be tumour suppressor genes. In present study, genes with decreased were screened for by serial analysis gene (SAGE) data and reverse transcription (RT)-polymerase chain reaction (PCR), CLDN18 (encoding claudin-18) was identified. Quantitative RT-PCR revealed that 13 (56.5%) 23 GCs. Immunostaining showed normal gastric mucosa Paneth cells duodenum expressed claudin-18 on cell...
Abstract Objective: To investigate the molecular genetic heterogeneity associated with t(6:9) in adenoid cystic carcinoma (ACC) and correlate findings patient clinical outcome. Experimental Design: Multimolecular techniques complemented massive pair-ended sequencing single-nucleotide polymorphism array analyses were used on tumor specimens from 30 new 52 previously analyzed fusion transcript–negative ACCs by reverse transcriptase PCR (RT-PCR). MYB mRNA expression level was determined...
Salivary gland adenoid cystic carcinoma (ACC) has heterogeneous clinical behavior. Currently, all patients are treated uniformly, and no standard-of-care systemic therapy exists for metastatic ACC. We conducted an integrated proteogenomic analyses of ACC tumors to identify dysregulated pathways propose a classification with therapeutic implications.RNA/DNA sequencing 54 flash-frozen salivary ACCs reverse phase protein array (RPPA) in 38 specimens were performed, validation by Western...
We conducted a phase II trial evaluating the efficacy of VEGFR inhibitor axitinib and PD-L1 avelumab in patients with recurrent/metastatic adenoid cystic carcinoma (R/M ACC).
Abstract BACKGROUND The authors purpose in this study was to clarify the difference terms of clinicopathologic features between gastric cancer (GC) with high numbers DNA methylated genes and CpG island methylator phenotype (CIMP)‐positive GC as originally defined. METHODS We analyzed methylation 12 tumor‐related ( hMLH1 , MGMT p16 INK4a CDH1 RAR ‐ beta HLTF RIZ1 TM FLNc LOX HRASLS HAND1 ) 75 samples from patients, 25 corresponding nonneoplastic mucosa 10 normal healthy young individuals by...
Abstract Regenerating islet‐derived family, member 4 (Reg IV) is a candidate marker for cancer and inflammatory bowel disease. In the present study, immunohistochemical analysis of Reg IV was performed in various human neoplastic ( n = 289) non‐neoplastic tissues. stomach, foveolar epithelium negative IV, whereas goblet cells intestinal metaplasia neuroendocrine at base expressed IV. Neuroendocrine small intestine colon showed strong expression weak or no Insulin‐producing beta endocrine...
To investigate the molecular events associated with activation of androgen receptor (AR) as a potential therapeutic target in patients salivary duct carcinoma (SDC).Comprehensive and expression analysis AR gene 35 tumor specimens (20 males 15 females) cell lines derived from SDC using Western blotting RT-PCR, FISH analysis, DNA sequencing was conducted. In vitro vivo animal studies were also performed.AR detected 70% tumors mainly nuclear homogenous both male female SDCs, although variable...
Abstract Pot1, a telomere end-binding protein in fission yeast and human, is proposed not only to cap telomeres but also recruit telomerase the ends of chromosomes. No study has been performed regarding Pot1 expression status human cancers. Thus, we examined POT1 mRNA 51 gastric cancer (GC) tissues evaluated length 3′ telomeric overhang signals 20 GC tissues. Quantitative reverse transcription-PCR analysis showed that levels tumor relative those nonneoplastic mucosa (T/N ratio) were...
// Ruli Gao 1,2 , Chunxia Cao 1 Min Zhang Maria-Cecilia Lopez 3 Yuanqing Yan 2 Zirong Chen Yoshitsugu Mitani 4 Li 5 Maria Zajac-Kaye 6 Bin Liu 7 Lizi Wu Rolf Renne Henry V. Baker Adel El-Naggar and Frederic J. Kaye Department of Medicine, Division Hematology Oncology, College University Florida, Gainesville, FL, USA Genetics & Genomics Graduate Program, Institute, Molecular Microbiology, Pathology, The Texas MD Anderson Cancer Center, Houston, TX, Computational Biology Bioinformatics,...
Adenoid cystic carcinoma (ACC) is an aggressive head and neck malignancy characterized by a t (6;9) translocation resulting in MYB-NFIB gene fusion or, more rarely, MYBL1 fusion. The true frequency clinical significance of these alterations are still unclear. Here, we have used tissue microarrays analyzed 391 ACCs 647 non-ACC salivary neoplasms to study the prevalence, expression, MYB/MYBL1 FISH immunohistochemistry. Alterations MYB or were found 78% cases, which 62% had 16% rearrangements....
Adenoid cystic carcinoma (ACC) is a heterogeneous malignancy, and no effective systemic therapy exists for metastatic disease. We previously described two prognostic ACC molecular subtypes with distinct therapeutic vulnerabilities, ACC-I ACC-II. In this study, we explored the tumor microenvironment (TME) using RNA-sequencing spatial biology to identify potential targets.
Background Salivary adenoid cystic carcinoma (ACC) is a rare relentlessly progressive malignant tumor. The molecular events associated with ACC tumorigenesis are poorly understood. Variable microRNAs (miRNA) have been correlated of several solid tumors but not in ACC. To investigate the association miRNAs development and/or progression ACC, we performed comparative analysis primary specimens and matched normal samples pooled salivary gland standard results clinicopathologic factors validated...
// Candace A. Frerich 1 , Kathryn J. Brayer 1, 2 Brandon M. Painter Huining Kang Yoshitsugu Mitani 3 Adel K. El-Naggar and Scott Ness Department of Internal Medicine, University New Mexico Health Sciences Center, Albuquerque, NM, USA Comprehensive Cancer Head Neck Pathology, Texas MD Anderson Houston, TX, Correspondence to: Ness, email: sness@salud.unm.edu Keywords: personalized medicine; precision biomarker; emt; bioinformatics Abbreviations: ACC: Adenoid cystic carcinoma; FFPE:...
Abstract Histone acetylation appears to play an important role in transcriptional regulation. Inactivation of chromatin by histone deacetylation is involved the repression several tumour suppressor genes, including p 21 WAF1/CIP1 . However, vivo status human cancers, gastric carcinoma, not well understood. This study shows that H3 WAF 1/ CIP 1 promoter region hypoacetylated and this hypoacetylation associated with reduced expression carcinoma specimens. Chromatin immunoprecipitation assays...
The <i>RUNX3</i> gene is a member of the Runt domain family transcription factors that are master regulators expression in major developmental pathways. Recently, lack function was found to be associated with genesis and progression gastric carcinoma. We studied methylation CpG islands by methylation-specific PCR 80 carcinoma specimens, 45 corresponding non-neoplastic mucosae, 7 cell lines. also measured levels mRNA 50 cases quantitative RT-PCR lines RT-PCR. Hypermethylation...
Abstract The retinoblastoma protein‐interacting zinc finger gene, RIZ1 (GenBank accession number U17838), is involved in chromatin‐mediated gene expression and also a target for frameshift mutation microsatellite‐unstable cancers. Methylation of the promoter CpG island has been shown to be common mechanism inactivating human liver breast We investigated levels mRNA 45 gastric carcinoma tissues by quantitative RT‐PCR cell lines RT‐PCR. In addition, we examined methylator phenotype (CIMP)...