A5088042175- Mosquito-borne diseases and control
- Laser Material Processing Techniques
- Integrated Circuits and Semiconductor Failure Analysis
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Advanced Surface Polishing Techniques
- Laser-induced spectroscopy and plasma
- Ion-surface interactions and analysis
- Neuroscience and Neural Engineering
- HIV Research and Treatment
- Advanced X-ray and CT Imaging
- Voice and Speech Disorders
- Autoimmune and Inflammatory Disorders Research
- Medical Image Segmentation Techniques
- Surface Roughness and Optical Measurements
- Industrial Vision Systems and Defect Detection
- Image and Object Detection Techniques
- Physical Unclonable Functions (PUFs) and Hardware Security
- Respiratory viral infections research
- Rangeland and Wildlife Management
- Optical Coherence Tomography Applications
- Airway Management and Intubation Techniques
- Complement system in diseases
- Terahertz technology and applications
- Additive Manufacturing Materials and Processes
University of Colorado Denver
2016-2025
University of Connecticut
2019-2025
Manhattan College
2023-2024
University of Maine
2023
Bipar
2023
Park University
2021
University of North Carolina at Chapel Hill
2019
Bowling Green State University
2016-2018
University of Colorado Anschutz Medical Campus
2016
La Trobe University
2009
Chikungunya virus (CHIKV) is an arthritogenic alphavirus that has re-emerged to cause large outbreaks of human infections worldwide. There are currently no approved antivirals for treatment CHIKV infection. Recently, we reported the ribonucleoside analog 4'-fluorouridine (4'-FlU) a highly potent inhibitor replication, and targets viral nsP4 RNA dependent polymerase. In mouse models, oral therapy with 4'-FlU diminished tissue burdens virus-induced disease signs. To provide critical evidence...
Chikungunya virus (CHIKV) and related alphaviruses cause epidemics of acute chronic musculoskeletal disease. To investigate the mechanisms underlying failure immune clearance CHIKV, we studied mice infected with an attenuated CHIKV strain (181/25) pathogenic parental (AF15561), which differ by five amino acids. Whereas AF15561 infection wild-type results in viral persistence joint tissues, 181/25 is cleared. In contrast, μMT lacking mature B cells suggesting that virus-specific antibody...
ABSTRACT Chikungunya virus (CHIKV) is a reemerging alphavirus that has caused epidemics of fever, arthralgia, and rash worldwide. There are currently no licensed vaccines or antiviral therapies available for the prevention treatment CHIKV disease. We conducted high-throughput, chemical compound screen identified digoxin, cardiac glycoside blocks sodium-potassium ATPase, as potent inhibitor infection. Treatment human cells with digoxin related glycoside, ouabain, resulted in dose-dependent...
Chikungunya virus (CHIKV) is an arbovirus capable of causing a severe and often debilitating rheumatic syndrome in humans. CHIKV replicates wide variety cell types mammals, which has made attributing pathologic outcomes to replication at specific sites difficult. To assess the contribution skeletal muscle cells pathogenesis, we engineered strain exhibiting restricted these via incorporation target sequences for cell-specific miR-206. This virus, term SKE, displayed diminished mouse model...
Alphaviruses are arthropod-borne viruses that represent a significant threat to public health at global level. While the formation of alphaviral nucleocapsid cores, consisting cargo nucleic acid and viral capsid protein, is an essential molecular process infection, precise interactions between two partners ill-defined. A CLIP-seq approach was used screen for candidate sites interaction Capsid protein genomic RNA Sindbis virus (SINV), model alphavirus. The data presented in this report...
Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that causes debilitating musculoskeletal pain and inflammation can persist for months to years after acute infection. Although studies of humans experimentally infected animals suggest CHIKV infection persists in tissues, the mechanisms this remain poorly understood. To evaluate further, we isolated from serum persistently Rag1-/- mice at day 28. When inoculated into naive wild-type (WT) mice, circulating strain displayed...
ABSTRACT Chikungunya virus (CHIKV) is an enveloped, positive-sense RNA that has re-emerged to cause millions of human infections worldwide. In humans, acute CHIKV infection causes fever and severe muscle joint pain. Chronic debilitating arthritis pain can persist for months years. To date, there are no approved antivirals against CHIKV. Recently, the ribonucleoside analog 4′-fluorouridine (4′-FlU) was reported as a highly potent orally available inhibitor SARS-CoV-2, respiratory syncytial...
Printed Circuit Board (PCB) design reconstruction is essential for addressing part obsolescence, intellectual property recovery, compliance, quality assurance, and enhancing national capabilities. Traditional methods PCB extraction, both non-geometry-based geometry-based, have limitations in accuracy, efficiency, scalability. This paper presents an automated approach, combining image processing machine learning, to achieve 3D semantic segmentation of X-ray Computed Tomography (X-ray CT)...
Alphaviruses are arthropod-transmitted RNA viruses that can cause arthralgia, myalgia, and encephalitis in humans. Since the role of cellular kinases alphavirus replication is unknown, we profiled kinetic changes host kinase abundance phosphorylation following chikungunya virus (CHIKV) infection fibroblasts.
The magnitude and duration of vertebrate viremia is a critical determinant arbovirus transmission, geographic spread, disease severity. We find that multiple alphaviruses, including chikungunya (CHIKV), Ross River (RRV), o'nyong 'nyong (ONNV) viruses, are cleared from the circulation mice by liver Kupffer cells, impeding viral dissemination. Clearance was independent natural antibodies or complement factor C3, instead relied on scavenger receptor SR-A6 (MARCO). Remarkably, lysine to arginine...
Chikungunya virus (CHIKV) causes acute and chronic rheumatologic disease. Pathogenic CHIKV strains persist in joints of immunocompetent mice, while the attenuated strain 181/25 is cleared by adaptive immunity. We analyzed draining lymph node (dLN) to define events lymphoid tissue that may contribute persistence or clearance. Acute infection resulted dLN enlargement germinal center (GC) formation, mice infected with pathogenic became highly disorganized depleted lymphocytes. Using encoding...
Abstract X-ray computed tomography (CT) is a powerful technique for non-destructive volumetric inspection of objects and widely used studying internal structures large variety sample types. The raw data obtained through an CT practice gray-scale 3D array voxels. This must undergo geometric feature extraction process before it can be interpretation purposes. Such conventionally done manually, but with the ever-increasing trend image sizes interest in identifying more miniature features,...
Cross sectioning is a critical sample preparation technique used in wide range of applications, that enables investigation buried layers and subsurface features or defects. State-of-the-art cross-sectioning methods, each have their own pros cons, but generally suffer from tradeoff between throughput accuracy. Mechanical methods are fast lack On the other hand, ion-based such as focused ion beam (FIB), offer high resolutions slow. Lasers, which can potentially improve this tradeoff, face...
Abstract Counterfeit products pose significant economic, security, and health risks. One approach to mitigate these risks involves establishing product provenance by tracing them back their manufacturing origins. However, current identification methods, such as barcodes RFIDs, have limitations that make vulnerable counterfeiting. Similarly, nonvolatile memories, physically unclonable functions, emerging techniques like Diamond Unclonable Security Tag DNA fingerprinting also own challenges....
The rapid, high-resolution material processing offered by ultrashort pulsed lasers enables a wide range of micro and nanomachining applications in variety disciplines. Complex laser jobs conducted on composite samples, require an awareness the type that is interacting with both for adjustment lasering process endpointing. This calls real-time detection materials. Several methods such as X-ray diffraction (XRD), photoelectron spectroscopy (XPS), energy dispersive X-Ray (EDS) can be used...
Acquiring detailed 3D images of samples is needed for conducting thorough investigations in a wide range applications. Doing so using nondestructive methods such as X-ray computed tomography (X-ray CT) has resolution limitations. Destructive methods, which work based on consecutive delayering and imaging the sample, face tradeoff between throughput resolution. Using focused ion beam (FIB) delayering, although high precision, low throughput. On other hand, mechanical that can offer fast are...