A5041008500- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Cystic Fibrosis Research Advances
- Microbial infections and disease research
- Entomopathogenic Microorganisms in Pest Control
- Nematode management and characterization studies
- Bacterial biofilms and quorum sensing
- Viral Infections and Immunology Research
- Transgenic Plants and Applications
- Evolution and Genetic Dynamics
- Genomics and Phylogenetic Studies
- Viral Infections and Vectors
- Respiratory viral infections research
- Phytase and its Applications
- Energy Harvesting in Wireless Networks
- Probiotics and Fermented Foods
- Plant Pathogenic Bacteria Studies
- Lipid Membrane Structure and Behavior
- Endoplasmic Reticulum Stress and Disease
- Vibrio bacteria research studies
- Cancer Research and Treatments
- Forest Insect Ecology and Management
- Antibiotic Resistance in Bacteria
- T-cell and B-cell Immunology
Dartmouth College
2024
Universidad de Valladolid
2023
University of Pittsburgh
2018-2022
Vanderbilt University
2013-2020
Children's Hospital of Pittsburgh
2017
Junta de Castilla y León
2015
ABSTRACT Chikungunya virus (CHIKV) is a reemerging alphavirus that has caused epidemics of fever, arthralgia, and rash worldwide. There are currently no licensed vaccines or antiviral therapies available for the prevention treatment CHIKV disease. We conducted high-throughput, chemical compound screen identified digoxin, cardiac glycoside blocks sodium-potassium ATPase, as potent inhibitor infection. Treatment human cells with digoxin related glycoside, ouabain, resulted in dose-dependent...
ABSTRACT Mammalian reovirus binds to cell-surface glycans and junctional adhesion molecule A enters cells by receptor-mediated endocytosis in a process dependent on β1 integrin. Within the endocytic compartment, undergoes stepwise disassembly, allowing release of transcriptionally active viral core into cytoplasm. To identify cellular mediators infectivity, we screened library small-molecule inhibitors for capacity block virus-induced cytotoxicity. In this screen, reovirus-induced cell...
Most viruses that replicate in the cytoplasm of host cells form neo-organelles serve as sites viral genome replication and particle assembly. These highly specialized structures concentrate proteins nucleic acids, prevent activation cell-intrinsic defenses, coordinate release progeny particles. Despite importance inclusion complexes replication, there are key gaps knowledge how these organelles mediate their functions. Reoviruses nonenveloped, double-stranded RNA (dsRNA) tractable...
Like most viruses that replicate in the cytoplasm, mammalian reoviruses assemble membranous neo-organelles called inclusions serve as sites of viral genome replication and particle morphogenesis. Viral inclusion formation is essential for infection, but how these organelles form not well understood. We investigated biogenesis reovirus inclusions. Correlative light electron microscopy showed endoplasmic reticulum (ER) membranes are contact with nascent inclusions, which by collections tubules...
Pseudomonas aeruginosa infections can be difficult to treat and new therapeutics are needed. Bacteriophage therapy is a promising alternative traditional antibiotics, but large numbers of isolated characterized phages lacking. We collected 23 diverse P. isolates from people with cystic fibrosis (CF) clinical infections, used them screen isolate over dozen aeruginosa-targeting hospital wastewater. Phages were genome sequencing, comparative genomics, lytic activity screening against all...
Pseudomonas aeruginosa notoriously adapts to the airways of people with cystic fibrosis (CF), yet how infection-site biogeography and associated evolutionary processes vary as lifelong infections progress remains unclear. Here we test hypothesis that early adaptations promoting aggregation influence evolutionary-genetic trajectories by examining longitudinal P. from sinuses six adults CF. Highly host-adapted lineages harbored mutator genotypes displaying signatures genome degradation recent...
Phage therapy is a therapeutic approach to treat multidrug-resistant (MDR) infections that employs lytic bacteriophages (phages) eliminate bacteria. Despite the abundant evidence for its success as an antimicrobial in Eastern Europe, there scarce data regarding effects on human host. Here, we aimed understand how phages interact with cells of airway epithelium, tissue site colonized by bacterial biofilms numerous chronic respiratory disorders. Using panel Pseudomonas aeruginosa and...
Mammalian orthoreoviruses (reoviruses) are nonenveloped viruses that replicate in cytoplasmic membranous organelles called viral inclusions (VIs) where progeny virions assembled. To better understand cellular routes of nonlytic reovirus exit, we imaged sites virus egress infected, nonpolarized human brain microvascular endothelial cells (HBMECs) and observed one or two distinct zones per cell at the basal surface. Transmission electron microscopy 3D tomography (ET) revealed clusters within...
Introduction and main objective Bursaphelenchus xylophilus , commonly known as pine wood nematode (PWN), is considered one of the greatest threats to European Asian pines. Regarding its management, most efforts have been directed toward control measures for major vector ( Monochamus spp.) screening genetic resistance in hosts. However, an integrated pest management strategy which also implements pinewood currently lacking. The aim this study was evaluate nematicidal effect two Beauveria...
Following infection, viruses synthesize nonstructural proteins that mediate viral replication and promote dissemination. Viruses from the family Reoviridae encode are required for formation of progeny viruses. Although different in diverge primary sequence, they functionally homologous appear to facilitate conserved mechanisms dsRNA virus replication. Using vitro cell culture approaches, we found mammalian reovirus protein σNS binds stabilizes RNA is genome synthesis. This work contributes...
ABSTRACT Phage therapy is a therapeutic approach to treat multidrug resistant infections that employs lytic bacteriophages (phages) eliminate bacteria. Despite the abundant evidence for its success as an antimicrobial in Eastern Europe, there scarce data regarding effects on human host. Here, we aimed understand how phages interact with cells of airway epithelium, tissue site colonized by bacterial biofilms numerous chronic respiratory disorders. We determined interactions between and...
The Pine Wood Nematode (PWN) Bursaphelenchus xylophilus is a severe forest pathogen in countries where it has been introduced and considered worldwide quarantine organism. In this study, protein markers for differentiating populations of nematode were identified by studying differences among four selected Iberian one American population. These compared quantitative proteomics (iTRAQ). From total 2860 proteins using the public database from B. genome project, 216 unambiguous significantly...
Abstract People with the genetic disorder cystic fibrosis (CF) harbor lifelong respiratory infections, morbidity and mortality frequently linked to chronic lung infections dominated by opportunistically pathogenic bacterium Pseudomonas aeruginosa . During CF a single clone of P. can persist for decades dominate end-stage disease due its propensity adaptively evolve environment, process termed “pathoadaptation”. Chronic rhinosinusitis (CRS), inflammation infection sinonasal space, is highly...
Abstract Pseudomonas aeruginosa infections can be difficult to treat and new therapeutic approaches are needed. Bacteriophage therapy is a promising alternative traditional antibiotics, but large numbers of isolated characterized phages lacking. We collected 23 genetically phenotypically diverse P. isolates from people with cystic fibrosis (CF) clinical infections, their genetic, phenotypic, prophage diversity. then used these screen isolate 14 -targeting hospital wastewater. Phages were...
Pseudomonas aeruginosa notoriously adapts to the airways of people with cystic fibrosis (CF), yet how infection site biogeography and associated evolutionary processes vary as lifelong infections progress remains unclear. Here we test hypothesis that early adaptations promoting aggregation influence evolutionary-genetic trajectories by examining longitudinal P. from sinuses six adults CF. Highly host-adapted lineages harbored mutator genotypes displaying signatures genome degradation recent...