A5055221785- Soybean genetics and cultivation
- Legume Nitrogen Fixing Symbiosis
- Bacterial biofilms and quorum sensing
- Cystic Fibrosis Research Advances
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Sinusitis and nasal conditions
- Antibiotic Resistance in Bacteria
- Mycobacterium research and diagnosis
- Gut microbiota and health
- Environmental DNA in Biodiversity Studies
- Molecular Communication and Nanonetworks
- Genomics and Phylogenetic Studies
- Photoreceptor and optogenetics research
- Respiratory viral infections research
- Inhalation and Respiratory Drug Delivery
- Evolution and Genetic Dynamics
- Bacillus and Francisella bacterial research
- Microbial infections and disease research
- Tuberculosis Research and Epidemiology
- Antimicrobial Resistance in Staphylococcus
- Ferroptosis and cancer prognosis
- Antimicrobial Peptides and Activities
- Infectious Diseases and Mycology
Dartmouth College
2023-2024
Carnegie Mellon University
2024
University of Pittsburgh
2018-2024
Stanford University
2023
University of Washington
2012-2020
ORCID
2020
Seattle University
2019
National Center for Emerging and Zoonotic Infectious Diseases
2011
Centers for Disease Control and Prevention
2011
Ferroptosis is a death program executed via selective oxidation of arachidonic acid–phosphatidylethanolamines (AA-PE) by 15-lipoxygenases. In mammalian cells and tissues, ferroptosis has been pathogenically associated with brain, kidney, liver injury/diseases. We discovered that prokaryotic bacterium, Pseudomonas aeruginosa, does not contain AA-PE can express lipoxygenase (pLoxA), oxidize host to 15-hydroperoxy-AA-PE (15-HOO-AA-PE), trigger in human bronchial epithelial cells. Induction...
The second messenger signaling molecule cyclic diguanylate monophosphate (c-di-GMP) drives the transition between planktonic and biofilm growth in many bacterial species. Pseudomonas aeruginosa has two surface sensing systems that produce c-di-GMP response to adherence. Current thinking field is once cells attach a surface, they uniformly respond by producing c-di-GMP. Here, we describe how Wsp system generates heterogeneity sensing, resulting physiologically distinct subpopulations of...
While considerable research has focused on the properties of individual bacteria, relatively little is known about how microbial interspecies interactions alter bacterial behaviors and pathogenesis. Staphylococcus aureus frequently coinfects with other pathogens in a range different infectious diseases. For example, coinfection by S. Pseudomonas aeruginosa occurs commonly people cystic fibrosis associated higher lung disease morbidity mortality. secretes numerous exoproducts that are to...
Abstract Background While the widespread initiation of elexacaftor/tezacaftor/ivacaftor (ETI) has led to dramatic clinical improvements among persons with cystic fibrosis (pwCF), little is known about how ETI affects respiratory mucosal inflammatory and physiochemical environment, or these changes relate lung function. Methods We performed a prospective, longitudinal study adults CF chronic rhinosinusitis (CF‐CRS) followed at our center ( n = 18). Endoscopic upper tract (paranasal sinus)...
Today, more than 90% of people with cystic fibrosis (pwCF) are eligible for the highly effective transmembrane conductance regulator (CFTR) modulator therapy called elexacaftor/tezacaftor/ivacaftor (ETI) and its use is widespread. Given drastic respiratory symptom improvement experienced by many post-ETI, clinical studies already underway to reduce number therapies, including antibiotic regimens, that pwCF historically relied on combat lung disease progression. Early suggest bacterial burden...
What are bacteria doing during "reversible attachment," the period of transient surface attachment when they initially engage a surface, besides attaching themselves to surface? Can an cell help any other attach? If so, does it all cells or employ more selective strategy either nearby (spatial neighbors) its progeny (temporal neighbors)? Using community tracking methods at single-cell resolution, we suggest answers these questions based on how reversible progresses sensing for Pseudomonas...
We measured in vitro phenotypes of early Pseudomonas aeruginosa isolates from children with cystic fibrosis an antibiotic eradication therapy trial. Isolates frequently exhibited associated chronic adaptation. Two were correlated failure to eradicate, representing promising candidate markers. Background. is a key respiratory pathogen people (CF). Due its association lung disease progression, initial detection P. CF cultures usually results treatment the goal eradication. exhibits many...
Phage therapy is a therapeutic approach to treat multidrug-resistant (MDR) infections that employs lytic bacteriophages (phages) eliminate bacteria. Despite the abundant evidence for its success as an antimicrobial in Eastern Europe, there scarce data regarding effects on human host. Here, we aimed understand how phages interact with cells of airway epithelium, tissue site colonized by bacterial biofilms numerous chronic respiratory disorders. Using panel Pseudomonas aeruginosa and...
Pseudomonas aeruginosa notoriously adapts to the airways of people with cystic fibrosis (CF), yet how infection-site biogeography and associated evolutionary processes vary as lifelong infections progress remains unclear. Here we test hypothesis that early adaptations promoting aggregation influence evolutionary-genetic trajectories by examining longitudinal P. from sinuses six adults CF. Highly host-adapted lineages harbored mutator genotypes displaying signatures genome degradation recent...
The cystic fibrosis (CF) respiratory tract harbors pathogenic bacteria that cause life-threatening chronic infections. Of these, Pseudomonas aeruginosa becomes increasingly dominant with age and is associated worsening lung function declining microbial diversity. We aimed to understand why P. dominates over other pathogens disease. Here, we show responds dynamic changes in iron concentration, often viral infection pulmonary exacerbations, become more competitive via expression of the TseT...
ABSTRACT Elexacaftor/tezacaftor/ivacaftor (ETI) therapy has revolutionized the treatment of cystic fibrosis (CF) for most affected individuals but effects on sinus microbiota are still unknown. Changes to airway in CF associated with disease state and alterations bacterial community after ETI initiation may require changes clinical management regimens. We collected swab samples from middle meatus an observational study 38 adults chronic rhinosinusitis (CRS) 2017 2021 captured therapy....
Healthcare-associated outbreaks and pseudo-outbreaks of rapidly growing mycobacteria (RGM) are frequently associated with contaminated tap water. A pseudo-outbreak Mycobacterium chelonae-M. abscessus in patients undergoing bronchoscopy was identified by 2 acute care hospitals. RGM specimens 28 patients, 25 whom resided the same skilled nursing facility (SNF). An investigation ruled out procedures, specimen collection, scope reprocessing at hospitals as sources transmission.To identify...
Chronic rhinosinusitis (CRS) is a common, yet underreported and understudied manifestation of upper respiratory disease in people with cystic fibrosis (CF). Recently developed standard care guidelines for the management CF CRS suggest treatment airway may ameliorate lower disease. We sought to determine whether changes sinus microbial community diversity specific taxa known cause lung are associated increased inflammation. performed 16S rRNA gene sequencing, supplemented cytokine analyses,...
Mucosa-associated biofilms are associated with many human disease states, but the host mechanisms promoting biofilm remain unclear. In chronic respiratory diseases like cystic fibrosis (CF), Pseudomonas aeruginosa establishes infection through formation. P . can be attracted to interspecies potassium currents emanating from biofilms. We hypothesized that could, similarly, sense and respond efflux airway epithelial cells (AECs) promote biofilm. Using co-culture imaging assays of grown in...
Abstract Water in healthcare environments can be a source for healthcare-associated infections (HAI). However, information on the exposure risk to opportunistic pathogens potable water distribution systems (PWDS) is lacking. Laboratory studies characterizing interaction of with biofilms are needed understand their role within facilities. A stable, repeatable, PWDS multi-species biofilm model comprising Sphingomonas paucimobilis, Methylobacterium sp., Delftia acidovorans, and Mycobacterium...
Abstract Background Shotgun sequencing of cultured microbial isolates/individual eukaryotes (whole-genome sequencing) and communities (metagenomics) has become commonplace in biology. Very often, sequenced samples encompass organisms spanning multiple domains life, necessitating increasingly elaborate software for accurate taxonomic classification assembled sequences. Results While many tools exist, SprayNPray offers a quick user-friendly, semi-automated approach, allowing users to separate...
ABSTRACT Phage therapy is a therapeutic approach to treat multidrug resistant infections that employs lytic bacteriophages (phages) eliminate bacteria. Despite the abundant evidence for its success as an antimicrobial in Eastern Europe, there scarce data regarding effects on human host. Here, we aimed understand how phages interact with cells of airway epithelium, tissue site colonized by bacterial biofilms numerous chronic respiratory disorders. We determined interactions between and...
Bacteria adapt to survive and grow in different environments. Genetic mutations that promote bacterial survival under harsh conditions can also restrict growth. The causes consequences of these adaptations have important implications for diagnosis, pathogenesis, therapy.We describe the isolation characterization an antibiotic-dependent, temperature-sensitive Pseudomonas aeruginosa mutant chronically infecting respiratory tract a cystic fibrosis (CF) patient, underscoring clinical challenges...
Abstract Shotgun sequencing of cultured microbial isolates/individual eukaryotes (whole-genome sequencing) and communities (metagenomics) has become commonplace in biology. Very often, sequenced samples encompass organisms spanning multiple domains life, necessitating increasingly elaborate software for accurate taxonomic classification assembled sequences. While many tools exist, SprayNPray offers a quick user-friendly, semiautomated approach, allowing users to separate contigs by taxonomy...
Abstract The second messenger signaling molecule cyclic diguanylate monophosphate (c-di-GMP) drives the transition from planktonic to biofilm growth in many bacterial species. Pseudomonas aeruginosa has two surface sensing systems that produce c-di-GMP response adherence. current thinking field is once cells attach a surface, they uniformly respond with elevated c-di-GMP. Here, we describe how Wsp system generates heterogeneity sensing, resulting physiologically distinct subpopulations of...