A5088271102- Insect symbiosis and bacterial influences
- Invertebrate Immune Response Mechanisms
- Plant Virus Research Studies
- Phytoplasmas and Hemiptera pathogens
- CRISPR and Genetic Engineering
- Bacterial biofilms and quorum sensing
- Insect Resistance and Genetics
- Seaweed-derived Bioactive Compounds
- Origins and Evolution of Life
- Herbal Medicine Research Studies
- Plant and Biological Electrophysiology Studies
- Protist diversity and phylogeny
- Mosquito-borne diseases and control
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
University of California, Berkeley
2019-2024
University of Michigan–Ann Arbor
2019
What are bacteria doing during "reversible attachment," the period of transient surface attachment when they initially engage a surface, besides attaching themselves to surface? Can an cell help any other attach? If so, does it all cells or employ more selective strategy either nearby (spatial neighbors) its progeny (temporal neighbors)? Using community tracking methods at single-cell resolution, we suggest answers these questions based on how reversible progresses sensing for Pseudomonas...
Toxin cargo genes are often horizontally transferred by phages between bacterial species and known to play an important role in the evolution of pathogenesis. Here, we show how these same have been from phage or bacteria animals resulted novel adaptations. We discovered that two widespread encoding toxins animal cells, cytolethal distending toxin subunit B ( cdtB ) apoptosis-inducing protein 56 kDa aip56) , were captured insect genomes through horizontal gene transfer phages. To study...
A growing body of evidence has underscored the role horizontal gene transfer (HGT) in animal evolution. Previously, we discovered encoding eukaryotic genotoxin cytolethal distending toxin B (cdtB) from pea aphid Acyrthosiphon pisum secondary endosymbiont (APSE) phages to drosophilid and nuclear genomes. Here, report cdtB genome gall-forming "swede midge" Contarinia nasturtii (Diptera: Cecidomyiidae) via HGT. We searched all available gall midge sequences for APSE-to-insect HGT events found...
Summary Immune systems are among the most dynamically evolving traits across tree of life, and long-lived macroparasites play an outsized role in shaping animal immunity. Even without adaptive immunity, insects have evolved potent innate immune strategies to neutralize such enemies, including nematodes parasitoid wasps. One strategy relies on endosymbioses between toxin-expressing bacteria. Here, we use genome editing Drosophila melanogaster retrace evolution two toxins — cytolethal...
Introduction: Species of the ananassae subgroup Drosophilidae are highly resistant to parasitoid wasp infections. We have previously shown that genes encoding Cytolethal Distending Toxin B (CdtB) and Apoptosis Inducing Protein 56 kDa (AIP56) were horizontally transferred these fly species from prokaryotes now instrumental in anti-parasitoid immune defense Drosophila ananassae. Here we describe a new family genes, which encode proteins with Hemolysin E domains, heretofore only identified...
Abstract A growing body of evidence points to a role for horizontal gene transfer (HGT) in the evolution animal novelties. Previously, we discovered encoding eukaryotic genotoxin cytolethal distending toxin B (CdtB) from Acyrthosiphon pisum Secondary Endosymbiont (APSE) bacteriophage drosophilid and aphid genomes. Here, report that cdtB is also found nuclear genome gall-forming ‘swede midge’ Contarinia nasturtii (Diptera: Cecidomyiidae). We subsequently searched sequences all available...
Abstract Despite possessing the machinery to sense, adhere to, and proliferate on surfaces, it is commonly observed that bacteria initially have a difficult time attaching surface. Before forming bacterial biofilm, planktonic exhibit random period of transient surface attachment known as “reversible attachment” which poorly understood. Using community tracking methods at single-cell resolution, we examine how reversible progresses during initial stages sensing. Pseudomonas aeruginosa strains...