A5024863500- Bacterial biofilms and quorum sensing
- Lipid Membrane Structure and Behavior
- Mass Spectrometry Techniques and Applications
- Advancements in Photolithography Techniques
- Electron and X-Ray Spectroscopy Techniques
- Vibrio bacteria research studies
- Ion-surface interactions and analysis
- Advanced Electron Microscopy Techniques and Applications
- Enzyme Structure and Function
- Plant and Biological Electrophysiology Studies
Yale University
2024-2025
Whitney Museum of American Art
2024
University of California, Los Angeles
2019-2020
California NanoSystems Institute
2019-2020
Abstract Biofilm formation by Vibrio cholerae facilitates environmental persistence, and hyperinfectivity within the host. is regulated 3’,5’-cyclic diguanylate (c-di-GMP) requires production of type IV mannose-sensitive hemagglutinin (MSHA) pilus. Here, we show that MSHA pilus a dynamic extendable retractable system, its activity directly controlled c-di-GMP. The interaction between c-di-GMP ATPase MshE promotes extension, whereas low levels correlate with enhanced retraction. Loss...
What are bacteria doing during "reversible attachment," the period of transient surface attachment when they initially engage a surface, besides attaching themselves to surface? Can an cell help any other attach? If so, does it all cells or employ more selective strategy either nearby (spatial neighbors) its progeny (temporal neighbors)? Using community tracking methods at single-cell resolution, we suggest answers these questions based on how reversible progresses sensing for Pseudomonas...
We report on the latest advancements in Microcrystal Electron Diffraction (3D ED/MicroED), as discussed during a symposium at National Center for CryoEM Access and Training housed New York Structural Biology Center. This snapshot describes cutting-edge developments various facets of field identifies potential avenues continued progress. Key sections discuss instrumentation access, research applications small molecules biomacromolecules, data collection hardware software, reduction finally...
At Yale University, the CryoEM Resource (YCR) core operates five high-resolution electron microscopes with expert staff microscopists providing scientific advice, training, and service to a diverse community of users. Recently, university has acquired new instrumentation perform diffraction experiments on an existing microscope. Additionally, Yale’s Chemical Biophysical Instrumentation Center (CBIC) its scientists provide expertise, in solution solid-state NMR, mass spectrometry, SAXS, x-ray...
Abstract Despite possessing the machinery to sense, adhere to, and proliferate on surfaces, it is commonly observed that bacteria initially have a difficult time attaching surface. Before forming bacterial biofilm, planktonic exhibit random period of transient surface attachment known as “reversible attachment” which poorly understood. Using community tracking methods at single-cell resolution, we examine how reversible progresses during initial stages sensing. Pseudomonas aeruginosa strains...