A5004425701- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Legume Nitrogen Fixing Symbiosis
- Mosquito-borne diseases and control
- Plant nutrient uptake and metabolism
- Aquaculture disease management and microbiota
- Neurobiology and Insect Physiology Research
- Research on Leishmaniasis Studies
- Coastal wetland ecosystem dynamics
- Photosynthetic Processes and Mechanisms
- Plant biochemistry and biosynthesis
- Insect and Arachnid Ecology and Behavior
- Insect-Plant Interactions and Control
- Insect and Pesticide Research
- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Aquaculture Nutrition and Growth
- CRISPR and Genetic Engineering
- Viral Infections and Vectors
- Antioxidant Activity and Oxidative Stress
- Trypanosoma species research and implications
- Seaweed-derived Bioactive Compounds
- Immune Cell Function and Interaction
- Insect Resistance and Genetics
- Herbal Medicine Research Studies
HUN-REN Szegedi Biológiai Kutatóközpont
2004-2025
Institute of Genetics
2013-2025
Hungarian Research Network
2023
Hungarian Academy of Sciences
2004-2019
Karlsruhe Institute of Technology
2006-2008
Isoprene (2-methyl-1,3-butadiene) emission varies diurnally in different species. In poplar (Populus spp.), it has recently been shown that the gene encoding synthesizing enzyme for isoprene, isoprene synthase (ISPS), displays diurnal variation expression. Working on shoot cultures of Grey × canescens) placed under a light regime phytochambers, we showed these variations PcISPS expression, measured by quantitative real-time polymerase chain reaction, are not only due to day-night changes,...
Toxin cargo genes are often horizontally transferred by phages between bacterial species and known to play an important role in the evolution of pathogenesis. Here, we show how these same have been from phage or bacteria animals resulted novel adaptations. We discovered that two widespread encoding toxins animal cells, cytolethal distending toxin subunit B ( cdtB ) apoptosis-inducing protein 56 kDa aip56) , were captured insect genomes through horizontal gene transfer phages. To study...
Parasitoid elimination in Drosophila melanogaster involves special hemocytes, called lamellocytes, which encapsulate the eggs or larvae of parasitoid wasps. The capsules are melanized, and metabolites melanization reaction may play a potential role killing. We have observed variation capacity different, commonly used D. strains, such as Canton-S, Oregon-R, BL5905, BL6326. In this work, we aimed to clarify possible connection between effectiveness capsule success following infection with...
We identified and characterized a so far unrecognized cell type, dubbed the multinucleated giant hemocyte (MGH), in ananassae subgroup of Drosophilidae. Here, we describe functional ultrastructural characteristics this novel blood type as well its characterization with set discriminative immunological markers. MGHs are encapsulating cells that isolate kill parasite without melanization. They share some properties but differ considerably from lamellocytes, Drosophila melanogaster, broadly...
The NimC1 molecule has been described as a phagocytosis receptor, and is being used marker for professional phagocytes, the plasmatocytes, in Drosophila melanogaster. In studies including tumor-biology, developmental biology, cell mediated immunity, monoclonal antibodies (P1a P1b) to antigen are used. As we observed that these did not react with plasmatocytes of several strains genetic combinations, molecular analysis was performed on structure nimC1 gene. found 2 deletions an insertion...
Cell mediated immunity of the honey bee (Apis mellifera) involves activity several hemocyte populations, currently defined by morphological features and lectin binding characteristics. The objective present study was to identify molecular markers capable characterizing subsets hemocytes. We developed employed monoclonal antibodies with restricted reactions functionally distinct subpopulations. Melanizing cells, known as oenocytoids, were an antibody prophenoloxidase, aggregating cells...
Coevolution of hosts and their parasites has shaped heterogeneity effector hemocyte types, providing immune defense reactions with variable effectiveness. In this work, we characterize hemocytes Drosophila willistoni, a species that evolved cellular system extensive variation high degree plasticity. Monoclonal antibodies were raised used in indirect immunofluorescence experiments to subpopulations, follow functional features differentiation. Pagocytosis parasitization assays determine the...
In specific plant organs, namely the root nodules of alfalfa, fixed nitrogen (ammonia) produced by symbiotic partner Sinorhizobium meliloti supports growth host in nitrogen-depleted environment. Here, we report that a derivative S. carrying mutation chromosomal ntrR gene induced with enhanced fixation capacity, resulting an increased dry weight and content alfalfa. The efficient is result higher expression level nifH gene, encoding one subunits nitrogenase enzyme, nifA, transcriptional...
Background Insects have specialized cell types that participate in the elimination of parasites, for instance, lamellocytes broadly studied species Drosophila melanogaster . Other drosophilids, such as ananassae and invasive Zaprionus indianus , multinucleated giant hemocytes, a syncytium blood cells encapsulation eggs or larvae parasitoid wasps. These can be formed by fusion hemocytes circulation originate from lymph gland. Their ultrastructure highly resembles mammalian megakaryocytes....
A toxin-antitoxin (TA)-like system (designated as bat/bto genes) was identified in Bradyrhizobium japonicum, based on sequence homology and similarities organization size to known TA systems. Deletion of the module resulted pleiotropic alterations cell morphology metabolism. The generation time mutant considerably decreased rich media. Atomic force microscopy revealed modified shape (shorter wider) softness cells. synthesis phosphatidylcholine completely blocked bacteria, vaccenic acid,...
Summary The symbiotic nitrogen‐fixing soil bacterium S inorhizobium meliloti carries a large number of toxin–antitoxin ( TA ) modules both on the chromosome and megaplasmids. One them, vapBC ‐5 module that belongs to type II systems was characterized here. It encodes an active toxin vapC , shown be controlled negatively by complex its own proteins. Different mutants genes exhibited diverse effects efficiency during interaction with host plant M edicago sativa . absence entire region had no...
Summary Immune systems are among the most dynamically evolving traits across tree of life, and long-lived macroparasites play an outsized role in shaping animal immunity. Even without adaptive immunity, insects have evolved potent innate immune strategies to neutralize such enemies, including nematodes parasitoid wasps. One strategy relies on endosymbioses between toxin-expressing bacteria. Here, we use genome editing Drosophila melanogaster retrace evolution two toxins — cytolethal...
Introduction: Species of the ananassae subgroup Drosophilidae are highly resistant to parasitoid wasp infections. We have previously shown that genes encoding Cytolethal Distending Toxin B (CdtB) and Apoptosis Inducing Protein 56 kDa (AIP56) were horizontally transferred these fly species from prokaryotes now instrumental in anti-parasitoid immune defense Drosophila ananassae. Here we describe a new family genes, which encode proteins with Hemolysin E domains, heretofore only identified...
Previously, a novel cell type, the multinucleated giant hemocyte (MGH) was identified in <i>ananassae</i> subgroup of Drosophilidae. These cells share several features with mammalian cells, syncytium macrophages formed during granulomatous inflammation. We were able to show that MGHs also differentiate <i>Zaprionus indianus</i>, an invasive species belonging <i>vittiger</i> family, highly resistant large number parasitoid wasp species. have classified...
Multinucleated giant hemocytes (MGHs) represent a novel type of blood cell in insects that participate highly efficient immune response against parasitoid wasps involving isolation and killing the parasite. Previously, we showed circulating MGHs have high motility interaction with rapidly triggers encapsulation. However, structural molecular mechanisms behind these processes remained elusive. Here, used detailed ultrastructural analysis live imaging to study encapsulation Drosophila...
Due to the evolutionary conservation of regulation hematopoiesis, Drosophila provides an excellent model organism study blood cell differentiation and hematopoietic stem (HSC) maintenance. The larvae melanogaster respond immune induction with production special effector cells, lamellocytes, which encapsulate subsequently kill invader. Lamellocytes differentiate as a result concerted action all three compartments larva: lymph gland, circulating hemocytes, sessile tissue. Within communication...
Drosophila is an extremely useful model organism for understanding how innate immune mechanisms defend against microbes and parasitoids. Large foreign objects trigger a potent cellular response in larva. In the case of endoparasitoid wasp eggs, this includes hemocyte proliferation, lamellocyte differentiation eventual encapsulation egg. The reaction involves attachment spreading hemocytes around egg, which requires cytoskeletal rearrangements, changes adhesion properties cell shape, as well...