Background Changes in the microbial populations on skin of animals have traditionally been evaluated using conventional microbiology techniques. The sequencing bacterial 16S rRNA genes has revealed that human is inhabited by a highly diverse and variable microbiome had previously not demonstrated culture-based methods. goals this study were to describe inhabiting different areas canine skin, compare healthy allergic dogs. Methodology/Principal Findings DNA extracted from superficial swabs (n...

To characterize the skin-associated fungal microbiota (mycobiota) in dogs, and to evaluate influence of body site, individual dog or health status on distribution fungi, next-generation sequencing was performed targeting internal transcribed spacer region. A total 10 dogs with no history skin disease were sampled at distinct sites consisting haired mucosal skin, 8 diagnosed allergies six commonly affected by allergic disease. Analysis similarities revealed that site not an influencing factor...

Background Next generation sequencing ( NGS ) studies have demonstrated a diverse skin‐associated microbiota and microbial dysbiosis associated with atopic dermatitis in people dogs. The skin of cats has yet to be investigated using techniques. Hypothesis/Objectives We hypothesized that the fungal healthy feline would similar dogs, predominance environmental fungi, present on allergic cats. Animals Eleven nine diagnosed one or more cutaneous hypersensitivity disorders, including flea bite,...

Background The skin is inhabited by a multitude of microorganisms. An imbalance these microorganisms associated with disease, however, the causal relationship between microbiota and disease remains unknown. To describe cutaneous bacterial cats determine whether dysbiosis occurs on allergic cats, surfaces various regions 11 healthy 10 were sampled. Methodology/Principal findings Genomic DNA was extracted from swabs sequenced using primers that target V4 region 16S rRNA. sequences revealed...

Previous research revealed the feline skin bacterial microbiota to be site-specific and fungal individual-specific. The effect of other factors, such as genotype environment, have not yet been studied in cats, but shown potentially important shaping cutaneous animals. Therefore, objectives this study were evaluate these factors on oral cavity. influence was assessed through analysis different cat breeds, environment comparison indoor outdoor cats. DNA extracted from swabs, next-generation...

Abstract Feline and canine atopic dermatitis are thought to have a similar immunopathogenesis. As with dogs, detection of allergen‐specific IgE in cat serum merely supports diagnosis feline atopy based on compatible history, clinical signs elimination other pruritic dermatoses. In this study, rapid screening immunoassay (Allercept ® E‐Screen 2nd Generation; Heska AG, Fribourg, Switzerland; ES2G) was compared complete‐panel assay ; AG; CP) healthy cats no history skin disease cats. The latter...

Dermatophytosis, also known as ringworm, is a contagious fungal skin disease affecting humans and animals worldwide. Persian cats exhibit severe forms of the more commonly than other breeds cat, including long-haired breeds. Certain types dermatophytosis in are reportedly caused by monogenic inborn errors immunity. The goal this study was to identify genetic variants contributing phenotype dermatophytosis. Whole-genome sequencing case control followed genome-wide association identified...

The pathogenesis of feline allergic dermatitis (FAD) is unclear, with several differences from in dogs and humans.To survey cytokine expression levels healthy cats affected or asthma.Formalin-fixed, paraffin-embedded skin biopsies 22 21 without were used for cutaneous assays. Serum was obtained 17 cats, 18 dermatitis, a presumptive diagnosis asthma.Cutaneous mRNA evaluated quantitative PCR [interleukin (IL)-31 IL-31 Receptor A] RNA situ hybridisation (ISH) [IL-5, IL-31, IL-31RA, IL-33...

These guidelines present a systematic approach to diagnosis, treatment, and management of allergic skin diseases in dogs cats. The describe detailed diagnosis treatment plans for flea allergy, food atopy feline atopic syndrome Management the patient entails multimodal with frequent ongoing communication client. Obtaining comprehensive history is crucial diseases, key questions ask when presented canine patients. Once obtained, physical examination should be performed, minimum dermatologic...

Abstract Ichthyoses are hereditary skin disorders characterized by the formation of scales and defects in outermost layer epidermis. In dogs, at least six different breed-specific ichthyoses including a relatively common PNPLA1-related autosomal recessive ichthyosis Golden Retrievers known. this study, we investigated 14 with that were not homozygous for mutant PNPLA1 allele suggesting genetically distinct new form ichthyosis. Histopathological examinations showed lamellar, orthokeratotic...

Gingival overgrowth is an uncommon adverse effect of cyclosporine administration in veterinary species. In people, gingival a common complication for immunosuppression, generally following transplant procedures. Azithromycin has been used successfully managing human patients when cannot be reduced or discontinued. This case series describes six dogs being administered various dermatologic diseases that developed overgrowth. The were prescribed systemic azithromycin, with without concurrent...

This case report documents the clinical appearance, diagnosis and novel treatment of a central Texas cat with cutaneous leishmaniosis. The presented linear erosion on right pinnal margin, an ulcerated exophytic nodule hock swelling in nostril. Cytological histopathological findings were consistent PCR confirmed presence Leishmania mexicana, species endemic to Texas. An epidemiological investigation was conducted by trapping sandflies from cat's environment. Sandflies collected identified as...

Various Staphylococcus species have been demonstrated to play important roles on the skin, including causing disease and protecting host from pathogens. Although culture-based studies isolated various spp. feline very little is known regarding species-level communities host.To describe staphylococcal inhabiting skin of healthy cats with allergic dermatitis.Skin swabs ear canal groin 11 10 (nonlesional) were obtained.DNA was extracted used for next-generation sequencing targeting V1-3 region...

Abstract Lime sulphur is a common topical treatment for dermatophytosis in animals. Until recently, single veterinary lime formulation was available. The purpose of this study to compare the efficacy eight products vitro growth inhibition Microsporum canis using isolated infected spore model. Infective M. spores were from hairs collected untreated cats. Hairs macerated Triton‐X solution and according previously published protocol. Equal volumes suspension solutions incubated 5 min plated...

The existence of antibodies against cross-reactive carbohydrate determinants (CCDs) has been studied extensively in humans, and more recently, dogs cats. These can reduce the specificity vitro serum allergen tests.To investigate prevalence anti-CCD immunoglobulin (Ig)E both allergic nonallergic horses as well evaluate its potential impact on testing.Twenty-one 21 horses.Sera were analysed for IgE utilising a commercial CHO enzyme-linked immunosorbent assay (ELISA). An specific Fc-ε receptor...

Abstract Background Total skin electron beam radiation therapy (TSEBT) is an effective treatment for primary diffuse cutaneous lymphomas in humans. While several techniques exist, they all require significant commitment of staff time and resources. In veterinary medicine, canine-specific strategies have been adapted delivered but deemed not “realistically” clinically implementable given the over 2.5 h plus per fraction or relegated to palliative intent. Leveraging these technologies helical...

Urticaria (hives) is a fairly common clinical reaction pattern in horses. Although often attributed to allergic causes, several other aetiologies can lead the manifestation of condition. A thorough and complete history, specifically as it relates dermatological disease, practitioner's most powerful tool successful diagnosis management. Advanced diagnostics such allergen‐specific immunoglobulin E serum or intradermal allergen tests are only helpful when an aetiology has been confirmed based...