A5032373411- Hair Growth and Disorders
- Skin and Cellular Biology Research
- Wnt/β-catenin signaling in development and cancer
- Virology and Viral Diseases
- Virus-based gene therapy research
- Veterinary Oncology Research
- Hedgehog Signaling Pathway Studies
- Infectious Diseases and Mycology
- Wound Healing and Treatments
- Vector-borne infectious diseases
- Viral Infections and Vectors
- Nail Diseases and Treatments
- Cancer and Skin Lesions
- Dermatology and Skin Diseases
- Burn Injury Management and Outcomes
- Infections and bacterial resistance
- Parasitic Diseases Research and Treatment
- Microbial infections and disease research
- Insect and Pesticide Research
- Neurofibromatosis and Schwannoma Cases
- Animal Virus Infections Studies
- Neuroblastoma Research and Treatments
- Rabies epidemiology and control
- Fungal Infections and Studies
- Mycobacterium research and diagnosis
Texas A&M University
2019-2025
Texas College
2021
Davidson College
2020
University of Bern
2007-2020
Gifu University
2020
Zoetis (United States)
2020
Royal Netherlands Academy of Arts and Sciences
2018-2019
University Hospital of Bern
2014-2019
Oncode Institute
2019
University Medical Center Utrecht
2018
Resource14 January 2019Open Access Transparent process Long-term expanding human airway organoids for disease modeling Norman Sachs Oncode Institute, Hubrecht Institute-KNAW and UMC Utrecht, The Netherlands Search more papers by this author Angelos Papaspyropoulos Domenique D Zomer-van Ommen Wilhelmina Children's Hospital Inha Heo Lena Böttinger Dymph Klay St. Antonius Nieuwegein, Fleur Weeber Cancer Amsterdam, Guizela Huelsz-Prince FOM Institute AMOLF, Nino Iakobachvili Maastricht...
Mammalian epidermal stem cells maintain homeostasis of the skin epidermis and contribute to its regeneration throughout adult life. While 2D mouse cell cultures have been established decades ago, a long-term, feeder cell- serum-free culture system recapitulating murine architecture has not available. Here we describe an organoid that allows genetically stable expansion cells. Our media combines atypically high calcium concentrations, activation cAMP, FGF, R-spondin signaling with inhibition...
Dermoid cysts are developmental abnormalities usually located at the dorsal body midline. Histologically, these lesions reduplicate skin, with associated collagen bundles, adnexal structures, and filled by keratin hairs. While have well-recognized breed anatomical predispositions in dogs, information feline patients is restricted to scattered case reports anecdotal data. Through a multi-institutional retrospective series, we aim describe clinical demographic features of this condition cats....
Abstract Background Ichthyoses represent a heterogeneous group of cornification disorders characterised by epidermal scaling. Objectives To describe the clinical, histopathological and genetic analysis Labrador retriever with nonepidermolytic ichthyosis, results population screening for newly detected PNPLA1 genomic duplication. Animals Two 7‐year‐old male littermates, 531 samples. Materials Methods Clinical analysis, whole genome sequencing digital PCR‐based genotyping were performed....
Canine distemper virus (CDV) causes in dogs a severe systemic infection, with high frequency of demyelinating encephalitis. Among the six genes transcribed by CDV, P gene encodes polymerase cofactor protein (P) as well two additional nonstructural proteins, C and V; these V was shown to act virulence factor. We investigated molecular mechanisms which products neurovirulent CDV A75/17 strain disrupt type I interferon (IFN-alpha/beta)-induced signaling that results establishment antiviral...
The hair follicle (HF) has a wide range of functions including thermoregulation, physical and immunological protection against external insults, sensory perception, social interactions, camouflage. One the most characteristic features HFs is that they self-renew during cycle (HC) throughout entire life an individual to continuously produce new hair. HC disturbances are common in humans comparable some alopecic disorders dogs. A normal maintained by follicular stem cells (SCs), which...
Ichthyoses are a heterogeneous group of inherited cornification disorders characterized by generalized dry skin, scaling and/or hyperkeratosis. Ichthyosis vulgaris is the most common form ichthyosis in humans and caused genetic variants FLG gene encoding filaggrin. Filaggrin key player formation stratum corneum, uppermost layer epidermis therefore crucial for barrier function. During terminal differentiation keratinocytes, precursor profilaggrin cleaved several proteases into filaggrin...
Long-standing reports of open sores on the hind legs moose ( Alces alces ) have been recorded in Alaska (as well as Canada, Europe, and Michigan), eliciting concerns about causes infection. We used histological genomic methods to investigate from 20 adult Kenai Peninsula, Alaska. paired this with thermal imagery molt scoring further describe sore formation understand its timing. Severe, ulcerative eosinophilic dermatitis was found all present, microfilariae within intraepidermal pustules...
Hereditary nasal parakeratosis (HNPK), an inherited monogenic autosomal recessive skin disorder, leads to crusts and fissures on the planum of Labrador Retrievers. We performed a genome-wide association study (GWAS) using 13 HNPK cases 23 controls. obtained single strong signal chromosome 2 (praw = 4.4×10−14). The analysis shared haplotypes among defined critical interval 1.6 Mb with 25 predicted genes. re-sequenced genome one case at 38× coverage detected 3 non-synonymous variants in...
Perturbations of epidermal and follicular homeostasis have been attributed to a variety skin diseases affecting dogs. The availability an in vitro system investigate these is important understand underlying pathomechanisms.To establish accurate reliable 3D canine keratinocyte organoids lay the basis for studying functional defects interfollicular epidermis (IFE) hair follicle (HF) morphogenesis, reconstitution differentiation that lead alopecic diseases.Skin biopsies were obtained from...
A 4-year-old, neutered female, domestic shorthair cat admitted to the animal hospital for recurrent constipation presumed be due post-traumatic injuries, went into shock with signs including fever and ataxia followed by stupor. On fifth day of hospitalization, developed severe, diffuse oedema ventral abdomen multifocal coalescing erythematous areas small vesicle formation. The results bacteriological cultures liver, spleen kidney specimens led diagnosis Acinetobacter baumannii sepsis....
Alopecia X is a hair cycle arrest disorder in Pomeranians. Histologically, kenogen and telogen follicles predominate, whereas anagen are sparse. The induction of relies on the activation follicle stem cells their subsequent proliferation differentiation. Stem cell function depends finely tuned interactions signaling molecules transcription factors, which not well defined dogs. We performed transcriptome profiling skin biopsies to analyze altered molecular pathways alopecia X. Biopsies from...
Whereas the genetic background of horn growth in cattle has been studied extensively, little is known about morphological changes developing fetal bud. In this study we histologically analyzed development buds bovine fetuses between ~70 and ~268 days pregnancy compared them with biopsies taken from frontal skin same fetuses. addition samples wild type (horned) bud region age-matched genetically hornless (polled) summary, multiple layers vacuolated keratinocytes visible early life already at...
Persistence in canine distemper virus (CDV) infection is correlated with very limited cell-cell fusion and lack of cytolysis induced by the neurovirulent A75/17-CDV compared to that cytolytic Onderstepoort vaccine strain. We have previously shown this difference was at least part due amino acid sequence (F) protein (P. Plattet, J. P. Rivals, B. Zuber, M. Brunner, A. Zurbriggen, R. Wittek, Virology 337:312-326, 2005). Here, we investigated molecular mechanisms CDV F underlying membrane...
Abstract Malignant peripheral nerve sheath tumours (MPNST) of a plexus or root cause significant morbidity and present treatment challenge. The surgical approach can be complex information is lacking on outcomes. objective this study was to describe complication rates oncologic outcomes for canine MPNST the brachial lumbosacral plexus. Dogs treated naïve with amputation/hemipelvectomy without laminectomy were retrospectively analysed. Oncologic disease free interval (DFI), overall survival...
Bernese mountain dogs are reported to have a shorter life expectancy than other breeds. A major reason for this has been assigned high tumour prevalence, especially of histiocytic sarcoma. The efforts made by the breeding clubs improve longevity with help genetic tests and value estimations impeded insufficiently reliable diagnoses regarding cause death. current standard post mortem examination in animals is performance an autopsy. In human forensic medicine, imaging modalities, such as...
Two varieties exist in the Chinese crested dog breed, namely hairless dogs presenting with hypotrichosis and dentition abnormalities, coated powderpuffs. Hairless are obligate heterozygotes for a FOXI3 mutation, this phenotype is classified as form of canine ectodermal dysplasia.We provide detailed histological description hair follicles their density three subphenotypes (true hairless, semi-coated powderpuffs) dogs. Apocrine exocrine glands skin other tissues were compared findings reported...
Functional hair follicle (HF) stem cells (SCs) are crucial to maintain the constant recurring growth of hair. In mice and humans, SC subpopulations with different biomarker expression profiles have been identified in discrete anatomic compartments HF. The rare studies investigating canine HF SCs shown similarities that mouse human SCs. aim our study was broaden current repertoire SC-associated markers their patterns dog. We combined analyses on levels CD34, K15, Sox9, CD200, Nestin, LGR5...
Abstract Ichthyoses are hereditary skin disorders characterized by the formation of scales and defects in outermost layer epidermis. In dogs, at least six different breed-specific ichthyoses including a relatively common PNPLA1-related autosomal recessive ichthyosis Golden Retrievers known. this study, we investigated 14 with that were not homozygous for mutant PNPLA1 allele suggesting genetically distinct new form ichthyosis. Histopathological examinations showed lamellar, orthokeratotic...
Summary Pollakisuria in adult goats can be caused by diseases of the urinary tract and distension parts genital leading to irritation bladder. Hydrometra is most common cause uterine usually resolved prostaglandin injections. But other pathologies uterus generate a similar syndrome. A dwarf goat was presented at clinic with history chronic pollakisuria tenesm. An initial ultrasonographic examination abdomen led suspicion hydrometra, but treatment injections were not successful. Blood samples...