A5042701818- Cancer Cells and Metastasis
- Advanced Breast Cancer Therapies
- Cell Adhesion Molecules Research
- Cancer Genomics and Diagnostics
- Breast Cancer Treatment Studies
- Neonatal Respiratory Health Research
- 3D Printing in Biomedical Research
- Estrogen and related hormone effects
- Renal Diseases and Glomerulopathies
- Platelet Disorders and Treatments
- Cellular Mechanics and Interactions
- Lung Cancer Research Studies
- Respiratory viral infections research
- Monoclonal and Polyclonal Antibodies Research
- Epigenetics and DNA Methylation
- Tumors and Oncological Cases
- Neuroendocrine Tumor Research Advances
- Viral gastroenteritis research and epidemiology
- Cancer Immunotherapy and Biomarkers
- Digestive system and related health
- Advanced Biosensing Techniques and Applications
- Tracheal and airway disorders
- Wnt/β-catenin signaling in development and cancer
- Cystic Fibrosis Research Advances
- Cancer therapeutics and mechanisms
Vertex Pharmaceuticals (United States)
2018-2022
Royal Netherlands Academy of Arts and Sciences
2012-2021
University Medical Center Utrecht
2014-2021
Oncode Institute
2017-2021
Hubrecht Institute for Developmental Biology and Stem Cell Research
2014-2020
Cancer Genomics Centre
2016
The Netherlands Cancer Institute
2006-2013
Resource14 January 2019Open Access Transparent process Long-term expanding human airway organoids for disease modeling Norman Sachs Oncode Institute, Hubrecht Institute-KNAW and UMC Utrecht, The Netherlands Search more papers by this author Angelos Papaspyropoulos Domenique D Zomer-van Ommen Wilhelmina Children's Hospital Inha Heo Lena Böttinger Dymph Klay St. Antonius Nieuwegein, Fleur Weeber Cancer Amsterdam, Guizela Huelsz-Prince FOM Institute AMOLF, Nino Iakobachvili Maastricht...
Significance Stem cells crucially depend on their complex microenvironment, also called niche. The niche is defined as an anatomic site, consisting of specialized cells. These anchor stem and provide the with physical protection essential growth maintenance signals. In murine small intestinal crypts, Paneth constitute important part cellular for Lgr5 + which they are intermingled. molecules such Wnt3, EGF, Notch ligands to maintain cell. There exists no typical cell in colon. Here, we show...
Novel reassortant avian influenza H7N9 virus and pandemic 2009 H1N1 (H1N1pdm) cause human infections, while H7N2 swine mainly infect birds pigs, respectively. There is no robust in vitro model for assessing the infectivity of emerging viruses humans. Based on a recently established method, we generated long-term expanding 3D airway organoids which accommodate four types epithelial cells: ciliated, goblet, club, basal cells. We report differentiation conditions increase ciliated cell numbers...
Abstract Recently, organoid technology has been used to generate a large repository of breast cancer organoids. Here we present an extensive evaluation the ability culture preserve complex stem/progenitor and differentiated cell types via long-term propagation normal human mammary tissues. Basal/stem luminal progenitor cells can differentiate in mature basal types, including ER+ that have challenging maintain culture. Cells associated with increased risk also be propagated. Single-cell...
The tetraspanin CD151 is a cell-surface molecule known for its strong lateral interaction with the laminin-binding integrin α3β1. Patients nonsense mutation in display end-stage kidney failure associated regional skin blistering and sensorineural deafness, mice lacking α3 subunit die neonatally because of severe abnormalities lung epithelia. We report generation Cd151-null that recapitulate renal pathology human patients, i.e., age they develop massive proteinuria caused by focal...
Re-epithelialization after skin wounding requires both migration and hyperproliferation of keratinocytes. Laminin-332 is deposited during over the provisional matrix. To investigate function laminin-332 binding integrin alpha3beta1 in wound re-epithelialization, we generated Itga3flox/flox; K14-Cre mice lacking alpha3 subunit specifically basal layer epidermis. These are viable but display several defects, including local inflammation, hair loss, basement membrane duplication microblistering...
ABSTRACT Multiple recent examples highlight how stem cells can self-organize in vitro to establish organoids that closely resemble their vivo counterparts. Single Lgr5+ mouse intestinal be cultured under defined conditions forming ever-expanding epithelial retain cell polarization, type diversity and anatomical organization of the epithelium. Although exhibiting a remarkable level self-organization, so called ‘mini-guts’ have closed cystic structure microscopic size. Here, we describe simple...
Integrins are transmembrane αβ glycoproteins that connect the extracellular matrix to cytoskeleton. The laminin-binding integrin α3β1 is expressed at high levels in lung epithelium and kidney podocytes. In podocytes, associates with tetraspanin CD151 maintain a functional filtration barrier. Here, we report on patient homozygous for novel missense mutation human ITGA3 gene, causing fatal interstitial disease congenital nephrotic syndrome. caused an alanine-to-serine substitution α3 subunit,...
Abstract Purpose: Although cyclin-dependent kinase 4 and 6 (CDK4/6) inhibitors significantly extend tumor response in patients with metastatic estrogen receptor–positive (ER+) breast cancer, relapse is almost inevitable. This may, part, reflect the failure of CDK4/6 to induce apoptotic cell death. We therefore evaluated combination therapy ABT-199 (venetoclax), a potent selective BCL2 inhibitor. Experimental Design: family member expression was assessed following treatment endocrine...
In vitro culture of intestinal tissue has been attempted for decades. Only recently did Sato et al. [Sato, T., Vries, R. G., Snippert, H. J., van de Wetering, M., Barker, N., Stange, D. E., Es, J. H., Abo, A., Kujala, P., Peters, P. (2009) Nature 459, 262-265] succeed in establishing long-term culture, demonstrating that cells expressing the Lgr5 gene can give rise to organoids with crypt-like domains similar those found vivo. these cultures, Paneth provide essential signals supporting stem...
Respiratory infections remain a major global health concern. Tuberculosis is one of the top 10 causes death worldwide, while with Non-Tuberculous Mycobacteria are rising globally. Recent advances in human tissue modeling offer unique opportunity to grow different "organs" vitro, including airway, that faithfully recapitulates lung architecture and function. Here, we have explored potential airway organoids (AOs) as novel system which assess very early steps mycobacterial infection. We reveal...