A5063408240- Hematopoietic Stem Cell Transplantation
- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Zebrafish Biomedical Research Applications
- Reproductive Biology and Fertility
- Cytomegalovirus and herpesvirus research
- Mesenchymal stem cell research
- Pregnancy and preeclampsia studies
- Immune Cell Function and Interaction
- Calcium signaling and nucleotide metabolism
- Cellular Mechanics and Interactions
- RNA Research and Splicing
- Autophagy in Disease and Therapy
- Renal and related cancers
- T-cell and B-cell Immunology
- interferon and immune responses
- Cancer Genomics and Diagnostics
- Histone Deacetylase Inhibitors Research
- RNA modifications and cancer
- Acute Myeloid Leukemia Research
École Polytechnique Fédérale de Lausanne
2016-2022
Bioengineering (Switzerland)
2021-2022
University Hospital of Geneva
2022
University Hospital of Zurich
2022
University of Geneva
2022
University of Zurich
2019
Haematopoietic stem cells (HSCs) differ from their committed progeny by relying primarily on anaerobic glycolysis rather than mitochondrial oxidative phosphorylation for energy production. However, whether this change in the metabolic program is cause or consequence of unique function HSCs remains unknown. Here we show that enforced modulation metabolism impacts HSC self-renewal. Lowering activity chemically uncoupling electron transport chain drives self-renewal under culture conditions...
In many leukemia patients, a poor prognosis is attributed either to the development of chemotherapy resistance by leukemic stem cells (LSCs) or inefficient engraftment transplanted hematopoietic stem/progenitor (HSPCs) into bone marrow (BM). Here, we build 3D in vitro model system organoids (BMOs) that recapitulate several structural and cellular components native BM. These are formed high-throughput manner from aggregation endothelial mesenchymal within hydrogel microwells. Accordingly,...
Abstract Gastruloids are three-dimensional embryonic organoids that reproduce key features of early mammalian development in vitro with unique scalability, accessibility, and spatiotemporal similarity to real embryos. Recently, we adapted the gastruloid culture conditions promote cardiovascular development. In this work, extended these capture blood through a combination immunophenotyping, detailed transcriptomics analysis, identification stem/progenitor cell potency. We uncovered emergence...
Abstract The transcription factor Yin Yang 1 (YY1) plays an important role in human disease. It is often overexpressed cancers and mutations can lead to a congenital haploinsufficiency syndrome characterized by craniofacial dysmorphisms neurological dysfunctions, consistent with brain development. Here, we show that Yy1 controls murine cerebral cortex development stage-dependent manner. By regulating wide range of metabolic pathways protein translation, maintains proliferation survival...
The in vitro expansion of long-term hematopoietic stem cells (HSCs) remains a substantial challenge, largely because our limited understanding the mechanisms that control HSC fate choices. Using single-cell multigene expression analysis and time-lapse microscopy, here we define gene signatures cell cycle hallmarks murine HSCs earliest multipotent progenitors (MPPs), analyze systematically single choices culture. Our revealed twelve differentially expressed genes marking quiescent state,...
Fatty acid β-oxidation (FAO), the breakdown of lipids, is a metabolic pathway used by various stem cells. FAO levels are generally high during quiescence and downregulated with proliferation. The endogenous metabolite malonyl-CoA modulates lipid metabolism as reversible inhibitor substrate for de novo lipogenesis. Here we assessed whether can be exploited to steer behavior hematopoietic stem/progenitor cells (HSPCs), quiescent clinical relevance. Treatment mouse HSPCs in vitro increases HSPC...
Gastruloids are three-dimensional embryonic organoids that reproduce key features of early mammalian development in vitro with unique scalability, accessibility, and spatiotemporal similarity to real embryos. Recently, we adapted gastruloid culture conditions promote cardiovascular development. In this work, extended these capture blood through a combination immunophenotyping, detailed transcriptomics analysis, identification stem/progenitor cell potency. We uncovered the emergence...
In many leukemia patients, a poor prognosis is attributed either to the development of chemotherapy resistance by leukemic stem cells (LSCs) or inefficient engraftment transplanted hematopoietic stem/progenitor (HSPCs) into bone marrow (BM). Here, we build 3D in vitro model system organoids (BMOs) that recapitulate several structural and cellular components native BM. These are formed high-throughput manner from aggregation endothelial mesenchymal within hydrogel microwells. Accordingly,...
Gastruloids are three-dimensional embryonic organoids that reproduce key features of early mammalian development in vitro with unique scalability, accessibility, and spatiotemporal similarity to real embryos. Recently, we adapted gastruloid culture conditions promote cardiovascular development. In this work, extended these capture blood through a combination immunophenotyping, detailed transcriptomics analysis, identification stem/progenitor cell potency. We uncovered the emergence...