A5010672265- Lung Cancer Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- Cancer Immunotherapy and Biomarkers
- Gastric Cancer Management and Outcomes
- Radiomics and Machine Learning in Medical Imaging
- Lung Cancer Research Studies
- Cancer Genomics and Diagnostics
- Esophageal Cancer Research and Treatment
- Pleural and Pulmonary Diseases
- Gastrointestinal Tumor Research and Treatment
- Esophageal and GI Pathology
- Tracheal and airway disorders
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Sepsis Diagnosis and Treatment
- Medical Imaging and Pathology Studies
- Salivary Gland Tumors Diagnosis and Treatment
- Neuroendocrine Tumor Research Advances
- Respiratory Support and Mechanisms
- Liver Disease Diagnosis and Treatment
- Genetic factors in colorectal cancer
- Intraperitoneal and Appendiceal Malignancies
- Ear and Head Tumors
- Cardiac, Anesthesia and Surgical Outcomes
- Heart Failure Treatment and Management
The Netherlands Cancer Institute
2016-2025
Leiden University Medical Center
2024-2025
Oncode Institute
2019-2025
Erasmus MC Cancer Institute
2024
Radboud University Nijmegen
2024
Catharina Ziekenhuis
2024
University Medical Center Utrecht
2021
University of Amsterdam
2020
Sanquin
2019
Amsterdam UMC Location Vrije Universiteit Amsterdam
2005-2016
IntroductionImmunotherapy is regarded as one of the major breakthroughs in cancer treatment. Despite its success, only a subset patients responds—urging quest for predictive biomarkers. We hypothesize that artificial intelligence (AI) algorithms can automatically quantify radiographic characteristics are related to and may therefore act noninvasive radiomic biomarkers immunotherapy response.Patients methodsIn this study, we analyzed 1055 primary metastatic lesions from 203 with advanced...
Malignant pleural mesothelioma (MPM) has limited treatment options and a poor outcome. Programmed death 1/programmed ligand 1 (PD-L1) checkpoint inhibitors have proven efficacious in several cancer types. Nivolumab is fully humanized monoclonal antibody against programmed with favorable toxicity profile. In MPM, the immune system considered to play an important role. We therefore tested nivolumab recurrent MPM.In this single-center trial, patients MPM received 3 mg/kg intravenously every 2...
Clinical implementation of tumor organoids for personalized medicine requires that pure can be reliably established. Here, we present our experience with organoid cultures from >70 non-small cell lung cancer (NSCLC) samples. We systematically evaluate several methods to identify purity established intrapulmonary tumors. Eighty percent lesions have a normal copy number profile, suggesting overgrowth by airway (AOs). This is further supported the failure detect mutations found in original...
Rationale: The clinical significance of diaphragm weakness in critically ill patients is evident: it prolongs ventilator dependency, and increases morbidity duration hospital stay. To date, the nature its underlying pathophysiologic mechanisms are poorly understood.Objectives: We hypothesized that muscle fibers mechanically ventilated display atrophy contractile weakness, ubiquitin–proteasome pathway activated diaphragm.Methods: obtained biopsies from 22 who received mechanical ventilation...
Cancer patients benefit from early tumor detection since treatment outcomes are more favorable for less advanced cancers. Platelets involved in cancer progression and considered a promising biosource detection, as they alter their RNA content upon local systemic cues. We show that tumor-educated platelet (TEP) RNA-based blood tests enable the of 18 types. With 99% specificity asymptomatic controls, thromboSeq correctly detected presence two-thirds 1,096 samples stage I–IV half 352 I–III...
Abstract Gastric and gastroesophageal junction (G/GEJ) cancers carry a poor prognosis, despite recent advancements, most patients die of their disease. Although immune checkpoint blockade became part the standard-of-care for with metastatic G/GEJ cancers, its efficacy impact on tumor microenvironment (TME) in early disease remain largely unknown. We hypothesized higher neoadjuvant immunotherapy plus chemotherapy nonmetastatic cancer. In phase 2 PANDA trial, previously untreated resectable...
Abstract Antibodies targeting the immune checkpoint molecules PD-1, PD-L1 and CTLA-4, administered alone or in combination with chemotherapy, are standard of care most patients metastatic non-small-cell lung cancers. When given before curative surgery, tumor responses improved event-free survival achieved. New antibody combinations may be more efficacious tolerable. In an ongoing, open-label phase 2 study, 60 biomarker-unselected, treatment-naive resectable cancer were randomized to receive...
Changes of portal hemodynamics with the progression chronic liver disease and changes caused by body posture physical exercise were investigated using an ultrasonic pulsed Doppler flowmeter in healthy adults patients persistent hepatitis, active cirrhosis. Portal venous velocity was significantly reduced cirrhosis without a large splenorenal shunt, compared normal subjects hepatitis. flow, contrast, only shunt other three groups; there no significant difference flow among latter four groups....
At present, palliative systemic chemotherapy is the standard treatment in Netherlands for gastric cancer patients with peritoneal dissemination. In contrast to lymphatic and haematogenous dissemination, dissemination may be regarded as locoregional spread of disease. Administering cytotoxic drugs directly into cavity has an advantage over since high concentrations can delivered limited toxicity. The combination a radical gastrectomy cytoreductive surgery (CRS) hyperthermic intraperitoneal...
Altered pulmonary hemodynamics and fluid flow-induced high shear stress (HSS) are characteristic hallmarks in the pathogenesis of arterial hypertension (PAH). However, contribution HSS to cellular vascular alterations PAH is unclear.We hypothesize that failing adaptation an essential part endothelial dysfunction all forms tested whether microvascular cells (MVECs) or (PAECs) from lungs patients with adapt if defect partakes remodeling vivo.PAH MVEC (n = 7) PAEC 3) morphology, function,...
Neuroendocrine neoplasms (NENs) comprise well-differentiated neuroendocrine tumors (NETs) and poorly differentiated carcinomas (NECs). Treatment options for patients with NENs are limited, in part due to lack of accurate models. We establish patient-derived tumor organoids (PDTOs) from pulmonary NETs derive PDTOs an understudied subtype NEC, large cell carcinoma (LCNEC), arising multiple body sites. maintain the gene expression patterns, intra-tumoral heterogeneity, evolutionary processes...
Abstract Cancer neoantigens that arise from tumor mutations are drivers of tumor-specific T cell responses, but identification cell-recognized in individual patients is challenging. Previous methods have restricted antigen discovery to selected HLA alleles, thereby limiting the breadth neoantigen repertoires can be uncovered. Here, we develop a genetic screening system allows sensitive CD4 + and CD8 across patients’ complete genotypes.
An ultrasonic sector scanner and pulsed Doppler flowmeter were combined to measure portal venous velocity in 19 patients with hypertension compared cineangiographic mapping of a droplet oil released into the vein. In 12 patients, measurements made before after pitressin [7] or ranitidine [5]. With method, maximum basal was 17.0 +/- 3.9 cm/sec., while average 8.5 2.7 significant difference (p less than 0.001). After pitressin, decreased 8.3 cm/sec. method 3.6 1.0 cineangiography. Ranitidine...
The clinical significance of diaphragm weakness in critically ill patients is evident: it prolongs ventilator dependency and increases morbidity, duration hospital stay, health care costs. mechanisms underlying are unknown, but might include mitochondrial dysfunction oxidative stress.We hypothesized that muscle fibers accompanied by impaired function structure, increased markers stress.To test these hypotheses, we studied contractile force, function, structure fibers. Fibers were isolated...
Background Checkpoint inhibitors provided sustained clinical benefit to metastatic lung cancer patients. Nonetheless, prognostic markers in settings are still under research. Imaging offers distinctive advantages, providing whole-body information non-invasively, while routinely available most clinics. We hypothesized that more can be extracted by employing artificial intelligence (AI) for treatment monitoring, superior 2D tumor growth criteria. Methods A cohort of 152 stage-IV non-small-cell...
Laparoscopic surgery has been shown to provide important advantages in comparison with open procedures the treatment of several malignant diseases, such as less perioperative blood loss and faster patient recovery. It also maintains similar results regard tumor resection margins oncological long-term survival. In gastric cancer role laparoscopic remains unclear. Current recommended for consists radical stomach, a free margin 5 6 cm from tumor, combined lymphadenectomy. The extent...
Abstract Background The role of cytoreductive surgery combined with hyperthermic intraperitoneal chemotherapy (HIPEC) in gastric cancer is unknown. This non-randomized dose-finding phase I–II study was designed to assess the safety and feasibility HIPEC, following systemic chemotherapy, patients limited peritoneal dissemination. maximum tolerated dose normothermic docetaxel combination a fixed oxaliplatin also explored. Methods Patients resectable cT3–cT4a adenocarcinoma metastases and/or...
Basophilic hepatic foci, nodules, and trabecular hepatocellular carcinomas, collectively referred to as focal lesions, were induced by single injections of 5.0 micrograms diethylnitrosamine (DEN) per gram body weight in 15-day-old C57BL/6J X C3HeB/FeJ F1 (B6C3 F1) mice. Groups eight experimental control mice killed at 3 days 1, 2, 4, 10, 20, 28, 36 41 weeks after injection. The only observable acute toxic effect DEN, a mild steatosis, was noted days, but this had disappeared 7 following...
Non-small cell lung cancer (NSCLC) is the second most prevalent type of cancer. With current treatment regimens, mortality rate remains high. Therefore, better therapeutic approaches are necessary. NSCLCs generally possess many genetic mutations and well infiltrated by T cells (TIL), making TIL therapy an attractive option. Here we show that from naive, stage I-IVa NSCLC tumors can effectively be isolated expanded, with similar efficiency as normal tissue. Importantly, 76% (13/17) tested...
Abstract Background Patients with a pathological complete response (pCR) after neoadjuvant chemoradiotherapy (nCRT) for oesophageal cancer may benefit from non-surgical management. The aim of this study was to determine the diagnostic performance visual assessment primary tumour nCRT on T2-weighted (T2W) and diffusion-weighted (DW) MRI. Methods locally advanced who underwent T2W- DW-MRI (1·5 T) before in two hospitals, between July 2013 September 2017, were included prospective study. Three...