A5065784242- Cardiovascular Function and Risk Factors
- Atherosclerosis and Cardiovascular Diseases
- Cardiac Fibrosis and Remodeling
- Cardiomyopathy and Myosin Studies
- Cardiovascular Disease and Adiposity
- Cardiovascular Effects of Exercise
- Advanced Glycation End Products research
- Cardiac Imaging and Diagnostics
- Viral Infections and Immunology Research
- Acute Myocardial Infarction Research
- Tissue Engineering and Regenerative Medicine
- Cell Adhesion Molecules Research
- Adipokines, Inflammation, and Metabolic Diseases
- Pulmonary Hypertension Research and Treatments
- Mesenchymal stem cell research
- Cardiac electrophysiology and arrhythmias
- Signaling Pathways in Disease
- Coronary Interventions and Diagnostics
- Cardiac Valve Diseases and Treatments
- Nitric Oxide and Endothelin Effects
- Complement system in diseases
- Blood properties and coagulation
- Wound Healing and Treatments
- Infective Endocarditis Diagnosis and Management
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Vrije Universiteit Amsterdam
2016-2025
Amsterdam University Medical Centers
2014-2025
Amsterdam UMC Location Vrije Universiteit Amsterdam
2015-2024
Amsterdam Neuroscience
2016-2024
University of Amsterdam
1991-2022
University Medical Center
2013-2018
University Hospital and Clinics
2013-2018
Reade
2016-2018
Sanquin
1999-2017
Leibniz Association
2017
Abstract Introduction Administration of mesenchymal stem cells (MSCs) represents a promising treatment option for patients suffering from immunological and degenerative disorders. Accumulating evidence indicates that the healing effects MSCs are mainly related to unique paracrine properties, opening opportunities secretome-based therapies. Apart soluble factors, release functional small RNAs via extracellular vesicles (EVs) seem convey essential features MSCs. Here we set out characterize...
Excessive diastolic left ventricular stiffness is an important contributor to heart failure in patients with diabetes mellitus. Diabetes presumed increase through myocardial deposition of collagen and advanced glycation end products (AGEs). Cardiomyocyte resting tension also elevates stiffness, especially normal ejection fraction (LVEF). The contribution fibrosis, AGEs, cardiomyocyte was assessed diabetic or reduced LVEF.Left endomyocardial biopsy samples were procured 28 LVEF 36 LVEF, all...
To support the clinical distinction between systolic heart failure (SHF) and diastolic (DHF), left ventricular (LV) myocardial structure function were compared in LV endomyocardial biopsy samples of patients with failure.Patients hospitalized for worsening classified as having SHF (n=22; ejection fraction (EF) 34+/-2%) or DHF LVEF 62+/-2%). No patient had coronary artery disease evidence infiltrative inflammatory disease. More a history arterial hypertension obese. Biopsy analyzed...
Data from the Women's Health Study show that serum levels of growth-differentiation factor-15 (GDF-15), a distant member transforming growth factor-beta superfamily, are an independent risk indicator for adverse cardiovascular events. However, cellular sources, upstream regulators, and functional effects GDF-15 in system have not been elucidated. We identified by cDNA expression array analysis as gene is strongly upregulated nitrosative stress cultured cardiomyocytes isolated 1- to 3-day-old...
Background— Prominent features of myocardial remodeling in heart failure with preserved ejection fraction (HFPEF) are high cardiomyocyte resting tension (F passive ) and hypertrophy. In experimental models, both reacted favorably to raised protein kinase G (PKG) activity. The present study assessed PKG activity, its downstream effects on F diameter, upstream control by cyclic guanosine monophosphate (cGMP), nitrosative/oxidative stress, brain natriuretic peptide (BNP). To discern altered...
Background Rises in circulating C-reactive protein (CRP), the prototypical acute-phase humans, correlate with clinical outcome patients myocardial ischemia and infarction. We hypothesized that these correlations might reflect active participation of CRP local inflammatory response ensuing jeopardized myocardium because on binding to a ligand, is able activate classic pathway complement, addition, complement activation has been shown occur locally infarcted myocardium. Methods Results To...
Monocytes are critical mediators of healing following acute myocardial infarction (AMI), making them an interesting target to improve repair. The purpose this study was a gain insight into the source and recruitment monocytes AMI in humans. Post-mortem tissue specimens myocardium, spleen bone marrow were collected from 28 patients who died at different time points after AMI. Twelve other causes served as controls. presence localization (CD14+ cells), their CD14+CD16– CD14+CD16+ subsets,...
Rationale: The clinical significance of diaphragm weakness in critically ill patients is evident: it prolongs ventilator dependency, and increases morbidity duration hospital stay. To date, the nature its underlying pathophysiologic mechanisms are poorly understood.Objectives: We hypothesized that muscle fibers mechanically ventilated display atrophy contractile weakness, ubiquitin–proteasome pathway activated diaphragm.Methods: obtained biopsies from 22 who received mechanical ventilation...
Background— Aortic stenosis (AS) and diabetes mellitus (DM) are frequent comorbidities in aging populations. In heart failure, DM worsens diastolic left ventricular (LV) dysfunction, thereby adversely affecting symptoms prognosis. Effects of on LV function were therefore assessed aortic stenosis, underlying myocardial mechanisms identified. Methods Results— Patients referred for valve replacement subdivided into patients with AS no (AS; n=46) (AS-DM; n=16). Preoperative Doppler...
Lack of gadolinium-contrast wash-in on first-pass perfusion imaging, early gadolinium-enhanced or late (LGE) cardiovascular magnetic resonance (CMR) imaging after revascularized ST-elevation myocardial infarction (STEMI) is commonly referred to as microvascular obstruction (MVO). Additionally, T2-weighted allows for the visualization infarct-related oedema and intramyocardial haemorrhage (IMH) within infarction. However, exact histopathological correlate contrast-devoid core its relation IMH...
Pro-coagulant and pro-inflammatory intramyocardial (micro)vasculature plays an important role in acute myocardial infarction (AMI). Currently, inhibition of serine protease dipeptidyl peptidase 4 (DPP4) receives a lot interest as anti-hyperglycemic therapy type 2 diabetes patients. However, DPP4 also possesses anti-thrombotic properties may behave immobilized anti-coagulant on endothelial cells. Here, we studied the expression activity human relation to prothrombogenic phenotype. Using...
Objective— Dysregulation of inflammatory adipokines by the adipose tissue plays an important role in obesity-associated insulin resistance. Pathways leading to this dysregulation remain largely unknown. We hypothesized that receptor for advanced glycation end products (RAGE) and ligand N ε -(carboxymethyl)lysine (CML) are increased and, moreover, activation CML–RAGE axis inflammation Approach Results— In study, we observed a strong CML accumulation expression RAGE obesity. confirmed cultured...
T cell autoreactivity is a hallmark of autoimmune diseases but can also benefit self-maintenance and foster tissue repair. Herein, we investigated whether heart-specific cells exert salutary or detrimental effects in the context myocardial infarction (MI), leading cause death worldwide. After screening more than 150 class-II-restricted epitopes, found that myosin heavy chain alpha (MYHCA) was dominant cardiac antigen triggering post-MI CD4+ activation mice. Transferred MYHCA614-629-specific...
Aims Although the autopsy is still gold standard for quality assessment of clinical diagnoses, rates have been declining over last decades to <10%. The aim this study was investigate value autopsies in high-tech medicine era by determining frequency discrepancies between and diagnoses. Methods We classified all adult cases (n=460), performed at Symbiant, Pathology Expert Centre, 2007 2012/2013, as having major, or minor discrepancy total concordance. roles possible contributory factors...
To characterize the clinical and MRI features of 2 families with adult-onset dominant leukoencephalopathy strokes identify underlying genetic cause.We applied pattern recognition, whole-exome sequencing, neuropathology.Based on brain imaging, 13 family members 40 years or older from were diagnosed disease; in 11 same age, was normal. In affected members, showed a that disproportionately severe compared to disease. The picture dominated by ischemic hemorrhagic strokes, slow late cognitive...
Effective tissue repair after myocardial infarction entails a vigorous angiogenic response, guided by incompletely defined immune cell–endothelial cell interactions. We identify the monocyte- and macrophage-derived cytokine METRNL (meteorin-like) as driver of postinfarction angiogenesis high-affinity ligand for stem factor receptor KIT (KIT tyrosine kinase). mediated effects in cultured human endothelial cells through KIT-dependent signaling pathways. In mouse model infarction, promoted...
Abstract Aims Precapillary pulmonary hypertension (precPH) results in increased right atrial (RA) stretch and pressure. The atrium is the major source of bone morphogenetic protein 10 (BMP10) adults, primarily produced by RA cardiomyocytes. aim this study was to investigate BMP10 expression heart systemic circulation identify potential triggers for secretion associated with precPH. Methods We examined mRNA expressions tissue. Circulating plasma levels were determined using ELISA....
Extent of MRI Delayed Enhancement Myocardial Mass Is Related to Right Ventricular Dysfunction in Pulmonary Artery HypertensionGerry P. McCann1 2, C. T. Gan3, Aernout M. Beek2, Hans W. Niessen4, Anton Vonk Noordegraaf3 and Albert van Rossum2Audio Available | Share