A5083048041- Immune cells in cancer
- Hematopoietic Stem Cell Transplantation
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- 3D Printing in Biomedical Research
- Immune Cell Function and Interaction
- Atherosclerosis and Cardiovascular Diseases
- Immune Response and Inflammation
- Microfluidic and Bio-sensing Technologies
- Acute Myeloid Leukemia Research
- Extracellular vesicles in disease
- Blockchain Technology Applications and Security
- Zebrafish Biomedical Research Applications
- Immunotherapy and Immune Responses
- Chemokine receptors and signaling
- MicroRNA in disease regulation
- Cell Image Analysis Techniques
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Cells and Metastasis
- Adipokines, Inflammation, and Metabolic Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell death mechanisms and regulation
- NF-κB Signaling Pathways
- Prion Diseases and Protein Misfolding
- Cancer, Stress, Anesthesia, and Immune Response
ETH Zurich
2013-2022
Board of the Swiss Federal Institutes of Technology
2016-2021
Center for Systems Biology
2009-2013
Harvard University
2009-2013
Massachusetts General Hospital
2009-2013
Brigham and Women's Hospital
2011
Dartmouth–Hitchcock Medical Center
2011
University of Lausanne
2009
Max Planck Society
2006
Monitoring Monocyte Reservoirs Monocytes are cells of the immune system that recruited to sites tissue injury and inflammation where they help resolve infection important for repair. The bone marrow blood believed be primary reservoirs from which monocytes mobilized after injury. Swirski et al. (p. 612 ; see Perspective by Jia Pamer ) now demonstrate spleen also serves as a critical reservoir during ischemic myocardial in very similar phenotype blood-derived injured heart, represent large...
Tumor-associated macrophages (TAMs) and tumor-associated neutrophils (TANs) can control cancer growth exist in almost all solid neoplasms. The cells are known to descend from immature monocytic granulocytic cells, respectively, which produced the bone marrow. However, spleen is also a recently identified reservoir of monocytes, play significant role inflammatory response that follows acute injury. Here, we evaluated splenic genetic mouse model lung adenocarcinoma driven by activation...
Background— Atherosclerotic lesions are believed to grow via the recruitment of bone marrow–derived monocytes. Among known murine monocyte subsets, Ly-6C high monocytes inflammatory, accumulate in preferentially, and differentiate. Here, we hypothesized that marrow outsources production during atherosclerosis. Methods Results— Using models atherosclerosis fate-mapping approaches, show hematopoietic stem progenitor cells progressively relocate from splenic red pulp, where they encounter...
Recognition and clearance of a bacterial infection are fundamental properties innate immunity. Here, we describe an effector B cell population that protects against microbial sepsis. Innate response activator (IRA) cells phenotypically functionally distinct, develop diverge from B1a cells, depend on pattern-recognition receptors, produce granulocyte-macrophage colony-stimulating factor. Specific deletion IRA activity impairs clearance, elicits cytokine storm, precipitates septic shock. These...
Monocytes serve as a central defense system against infection and injury but can also promote pathological inflammatory responses. Considering the evidence that monocytes exist in at least two subsets committed to divergent functions, we investigated whether distinct factors regulate balance between monocyte subset responses vivo. We identified microRNA (miRNA), miR-146a, which is differentially regulated both mouse (Ly-6Chi/Ly-6Clo) human (CD14hi/CD14loCD16+) subsets. The single miRNA...
Tumor-associated macrophages (TAMs) invade the tumor stroma in many cancers, yet their role is incompletely understood. To visualize and better understand these critical cells progression, we screened a portfolio of rationally selected, injectable agents to image endogenous TAMs ubiquitously three different cancer models (colon carcinoma, lung adenocarcinoma, soft tissue sarcoma). AMTA680, functionally derivatized magneto-fluorescent nanoparticle, labeled subset myeloid with an "M2"...
To evaluate myeloperoxidase (MPO) as a newer therapeutic target and bis-5-hydroxytryptamide-diethylenetriaminepentaacetate-gadolinium (Gd) (MPO-Gd) an imaging biomarker for demyelinating diseases such multiple sclerosis (MS) by using experimental autoimmune encephalomyelitis (EAE), murine model of MS.Animal experiments were approved the institutional animal care committee. EAE was induced in SJL mice proteolipid protein (PLP), treated with either 4-aminobenzoic acid hydrazide (ABAH), 40...
Cells receive a multitude of signals from the environment, but how they process simultaneous signaling inputs is not well understood. Response to infection, for example, involves parallel activation multiple Toll-like receptors (TLRs) that converge on nuclear factor κB (NF-κB) pathway. Although we increasingly understand inflammatory responses isolated signals, it clear cells co-occur in physiological settings. We therefore examined bacterial infection scenario involving co-stimulation TLR4...
Monocytes are circulating macrophage and dendritic cell precursors that populate healthy diseased tissue. In humans, monocytes consist of at least two subsets whose proportions in the blood fluctuate response to coronary artery disease, sepsis, viral infection. Animal studies have shown specific shifts monocyte subset repertoire either exacerbate or attenuate suggesting a role for as biomarkers therapeutic targets. Assays therefore needed can selectively rapidly enumerate their subsets. This...
To explore the capacity of human CD1⁺CD16⁺⁺ and CD14⁺⁺CD16⁻ monocytes to phagocyte iron-oxide nanoparticles in vitro.Human were labeled with four different magnetic nanoparticle preparations (Ferumoxides, SHU 555C, CLIO-680, MION-48) exhibiting distinct properties cellular uptake was quantitatively assessed by flow cytometry, fluorescence microscopy, atomic absorption spectrometry Magnetic Resonance Imaging (MRI). Additionally we determined whether resulted phenotypic changes cell surface...
Since its launch just over a decade ago by the cryptocurrency Bitcoin, distributed ledger technology (DLT) blockchain has followed breathtaking trajectory into manifold application spaces. This study aper analyses how key factors underpinning success of this ground-breaking “Internet value” technology, such as staking collateral (“skin in game”), competitive crowdsourcing, crowdfunding, and prediction markets, can be applied to substantially innovate legacy organization science, research,...
Dynamic environments determine cell fate decisions and function. Understanding the relationship between extrinsic signals on cellular responses requires ability to dynamically change environmental inputs in vitro, while continuously observing individual cells over extended periods of time. This is challenging for nonadherent cells, such as hematopoietic stem progenitor because media flow displaces disturbs preventing culture tracking single cells. Here, we present a programmable microfluidic...
Open microfluidic cell culturing devices offer new possibilities to simplify loading, culturing, and harvesting of individual cells or microtissues due the fact that liquids cells/microtissues are directly accessible. We present a complete workflow for handling microtissue spheroids, which is based on hanging-drop network concept: The open seamlessly combined with fluorescence-activated sorting (FACS), so cells, including stem can be sorted into specified compartments in fully automated way...
An overwhelming majority of experts has been flagging for decades that "Saving the Planet" requires immediate, persistent and drastic action to curb a variety catastrophic risks over 21st century. However, despite compelling evidence range suggested solutions, transnational coordination effective measures protect our biosphere continues fall short. To remedy, we propose novel platform addressing central issue affording trust, transparency truth while minimizing administrative overheads. This...
In a gradient of chemoattractant, Dictyostelium cells are orientated with their front directed toward the source and tail pointing into opposite direction. The region is specified by polymerization actin recruitment filamentous myosin‐II. We have dissected these responses exposing to an upshift cyclic AMP. A sharp rise fall polymerized within 10 s accompanied proteins involved in turning on or off. cortical accumulation myosin‐II starts when response has declined, supporting concept...
We outline the concept of an open technology platform which builds upon a publicly accessible library fluidic designs, manufacturing processes and experimental characterisation, as well virtualisation by 'digital twin". Backed rapidly emerging Web3 "Blockchain", we significantly extend traditional approaches to effectively incentivise broader participation interdisciplinary 'value network' diverse players. Ranging from skilled individuals more established research institutions companies with...