A5060733335- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- Tissue Engineering and Regenerative Medicine
- Hematological disorders and diagnostics
- Single-cell and spatial transcriptomics
- Electrospun Nanofibers in Biomedical Applications
- Cell Image Analysis Techniques
- 3D Printing in Biomedical Research
- Immune cells in cancer
- Fibroblast Growth Factor Research
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- Orthopaedic implants and arthroplasty
- Virus-based gene therapy research
- Hip disorders and treatments
- Medical and Biological Sciences
- Osteoarthritis Treatment and Mechanisms
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- Advanced Fluorescence Microscopy Techniques
- Management of metastatic bone disease
- Circadian rhythm and melatonin
- Telomeres, Telomerase, and Senescence
- Vitamin K Research Studies
Ottawa Hospital
2020-2025
Ottawa Hospital Research Institute
2020-2025
University of Ottawa
2012-2024
ETH Zurich
2013-2019
Helmholtz Zentrum München
2011-2018
Board of the Swiss Federal Institutes of Technology
2018
McGill University
2006-2011
Jewish General Hospital
2006-2011
Université de Montréal
1982-2008
Hôpital Saint-Luc
1982
The modification of glutamic acid residues to gamma-carboxyglutamic (Gla) is a post-translational catalyzed by the vitamin K-dependent enzyme gamma-glutamylcarboxylase. Despite ubiquitous expression gamma-carboxylation machinery in mammalian tissues, only 12 Gla-containing proteins have so far been identified humans. Because bone tissue second most abundant source after liver, we sought identify Gla secreted marrow-derived mesenchymal stromal cells (MSCs). We used proteomics approach screen...
Significance The development of an in vitro human bone marrow (BM) tissue appears essential to compile information on hematopoiesis. Conventional systems fail at both capturing the complexity niche while allowing maintenance functional hematopoietic stem cells (HSCs). Here, we report a 3D analogue perfusion-based bioreactor system, partially recapitulating structural, compositional, and organizational features native osteoblastic environment. engineered supports some progenitor cell (HSPC)...
Liver regeneration is the common mechanism whereby a patient recovers form liver injury. In western world, ethanol single most important aetiological factor associated with disease, and it appears crucial to determine if interferes regeneration. We studied response 70% hepatectomy in 240 rats receiving nutritionally adequate diet containing 36% of their calories as for three weeks pair-fed controls liquid where isocalorically replace carbohydrates. Criteria were: incorporation 3H-thymidine...
Vascularization is critical for skull development, maintenance, and healing. Yet, there remains a significant knowledge gap in the relationship of blood vessels to cranial skeletal progenitors during these processes. Here, we introduce quantitative 3D imaging platform enable visualization analysis high-resolution data sets (>100 GB) throughout entire murine calvarium. Using this technique, provide single-cell resolution maps vessel phenotypes frontoparietal bones. Through sets, demonstrate...
Mesenchymal stromal cells (MSCs) display robust reparative properties through their ability to limit apoptosis, enhance angiogenesis, and direct positive tissue remodeling. However, low in vivo survival of transplanted limits overall effectiveness significantly affects clinical usage. Consequently, identifying strategies improve cell are a priority. One explanation for is that MSCs often into ischemic tissue, such as infarcted myocardium, where there poor blood supply oxygen tension....
DNA damage can lead to the induction of cellular senescence. In particular, we showed that exposure ionizing radiation (IR) leads senescence bone marrow-derived multipotent stromal cells (MSC) and osteoblast-like (OB–SC), a phenotype associated with loss. The mechanism by which IR dysfunction is not fully understood. One possibility involves damage-induced limits regeneration progenitor cells. Another entails arises from inability accumulating senescent fulfill their physiological function....
Bladder replacement or augmentation is required in congenital malformations following trauma cancer. The current surgical solution involves enterocystoplasty but associated with high complication rates. Strategies for bladder tissue engineering are thus actively sought to address this unmet clinical need. Because of the poor efficacy synthetic polymers, use acellular matrix (BAM) has been proposed. Indeed when cellular components removed from xenogenic allogeneic bladders, extracellular...
Abstract Osteoarthritis (OA) represents a multifaceted pathology characterized by intricate signaling across various joint tissues, where the sub-synovial adipose tissue (ssAT) has been suggested to play diverse roles, from serving as stem cell reservoir, mechanosensing, neuroendocrine organ, modulating inflammation. In this study, we aimed uncouple cellular and molecular alterations within human hip ssAT (the pulvinar) linked OA aging, elucidating distinct contributions of disease onset...
Embryonic stem cells (ESCs) display heterogeneous expression of pluripotency factors such as Nanog when cultured with serum and leukemia inhibitory factor (LIF). In contrast, dual inhibition the signaling kinases GSK3 MEK (2i) converts ESC cultures into a state more uniform high expression. However, it is so far unclear whether 2i acts through an inductive or selective mechanism. Here, we use continuous time-lapse imaging to quantify dynamics death, proliferation, in mouse ESCs after...
We previously demonstrated that erythropoietin (EPO)-secreting mesenchymal stromal cells (MSC) can be used for the long-term correction of renal failure-induced anemia. The present study provides evidence coimplantation insulin-like growth factor I (IGF-I)-overexpressing MSC (MSC-IGF) improves MSC-based gene therapy anemia by providing paracrine support to EPO-secreting (MSC-EPO) within a subcutaneous implant. IGF-I receptor RNA expression in murine was RT-PCR. Functional protein confirmed...
The generation of humanized ectopic ossicles (hOss) in mice has been proposed as an advanced translational and fundamental model to study the human hematopoietic system. approach relies on presence bone marrow-derived mesenchymal stromal cells (hMSCs) supporting engraftment transplanted stem progenitor (HSPCs). However, functional distribution hMSCs within microenvironment remains be investigated. Here, we combined genetic tools quantitative confocal microscopy engineer subsequently analyze...