A5019480664- Mitochondrial Function and Pathology
- Neuroscience and Neuropharmacology Research
- Trigeminal Neuralgia and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Ion channel regulation and function
- Neurosurgical Procedures and Complications
- Neurogenesis and neuroplasticity mechanisms
- Facial Nerve Paralysis Treatment and Research
- Microtubule and mitosis dynamics
- MicroRNA in disease regulation
- Cellular transport and secretion
- Neuroinflammation and Neurodegeneration Mechanisms
- ATP Synthase and ATPases Research
- Head and Neck Surgical Oncology
- S100 Proteins and Annexins
- Genetic Neurodegenerative Diseases
- Meningioma and schwannoma management
- Traumatic Brain Injury and Neurovascular Disturbances
- Genetics and Neurodevelopmental Disorders
- Diversity and Career in Medicine
- Metabolism and Genetic Disorders
- Head and Neck Cancer Studies
- Child Abuse and Related Trauma
- Cerebral Venous Sinus Thrombosis
- PI3K/AKT/mTOR signaling in cancer
Harvard University
2012-2018
Massachusetts General Hospital
2012-2018
Center for Systems Biology
2012-2018
Radiology Associates
2018
Johns Hopkins Hospital
2016
Johns Hopkins University
2013-2016
Johns Hopkins Medicine
2013
Massachusetts Institute of Technology
2003-2010
Howard Hughes Medical Institute
2003-2009
Boston Children's Hospital
2004
The molecular mechanisms that determine the size and complexity of neuronal dendritic tree are unclear. Here, we show phosphoinositide-3′ kinase (PI3K)–Akt–mammalian target rapamycin (mTOR) signaling pathway promotes growth branching dendrites in cultured hippocampal neurons. Constitutively active mutants Ras, PI3K, Akt, or RNA interference (RNAi) knockdown lipid phosphatase PTEN (phosphatase tensin homolog deleted on chromosome Ten), induced elaboration was blocked by mTOR inhibitor and/or...
Mammalian Drp1 is a dynamin-like GTPase required for mitochondrial fission. Although it exists primarily as cytosolic homo-tetramer in vivo, can also self-assemble into higher order structures on the outer membrane, where proper division. Functional studies and sequence comparisons have revealed four different structural domains Drp1, comprising N-terminal GTP-binding, middle, insert B, C-terminal effector (GED) domains. Here we describe an intramolecular interaction within between GED...
Homeostatic plasticity mechanisms stabilize the activity of a neuron or neuronal circuit during prolonged periods increased network and have been proposed to function in prevention epilepsy. How homeostatic is achieved at molecular level hyperactivity states general, epileptiform particular, unclear. Using organotypic hippocampal slice cultures as model system, we found that protein kinase Polo-like 2 (Plk2) was induced required for activity-dependent reduction membrane excitability...
To evaluate myeloperoxidase (MPO) as a newer therapeutic target and bis-5-hydroxytryptamide-diethylenetriaminepentaacetate-gadolinium (Gd) (MPO-Gd) an imaging biomarker for demyelinating diseases such multiple sclerosis (MS) by using experimental autoimmune encephalomyelitis (EAE), murine model of MS.Animal experiments were approved the institutional animal care committee. EAE was induced in SJL mice proteolipid protein (PLP), treated with either 4-aminobenzoic acid hydrazide (ABAH), 40...
OBJECT The authors compared the efficacy of combining 2D+3D CT reconstructions with standard 2D images in diagnosis linear skull fractures children head trauma. METHODS This was a retrospective evaluation consecutive studies presenting Two experienced pediatric neuroradiologists consensus created reference. Three readers independently evaluated alone and then combination 3D for fractures. Sensitivity specificity utilizing were measured less than 2 years age all analysis by 3 readers. RESULTS...
Long-term depression (LTD) is a long-lasting activity-dependent decrease in synaptic strength. NMDA receptor (NMDAR)–dependent LTD, an extensively studied form of involves the endocytosis AMPA receptors (AMPARs) via protein dephosphorylation, but underlying mechanism has remained unclear. We show here that regulated interaction endocytic adaptor RalBP1 with two proteins, small GTPase RalA and postsynaptic scaffolding PSD-95, controls NMDAR-dependent AMPAR during LTD. NMDAR activation...
The ubiquitin-proteasome pathway (UPP) regulates synaptic function, but little is known about specific UPP targets and mechanisms in mammalian synapses. We report here that the SCFβ-TRCP complex, a multisubunit E3 ubiquitin ligase, postsynaptic spine-associated Rap GTPase activating protein (SPAR) for degradation neurons. SPAR by depended on activity-inducible kinase Polo-like 2 (Plk2). In presence of Plk2, physically associated with complex through canonical phosphodegron. hippocampal...
To determine if myeloperoxidase (MPO) is involved in epileptogenesis and molecular nuclear imaging can be used to noninvasively map inflammatory changes epileptogenesis.The animal human studies were approved by the institutional review boards. Pilocarpine-induced epileptic mice treated with 4-aminobenzoic acid hydrazide (n = 46), a specific irreversible MPO inhibitor, or saline 42). Indium-111-bis-5-hydroxytryptamide-diethylenetriaminepentaacetate was image brain activity 6 groups; n 5 sham...
Thin-section MR imaging through the posterior fossa is frequently used for trigeminal neuralgia. Typical heavily T2-weighted methods yield high anatomic detail and contrast between CSF neurovascular structures, but poor vessels nerves. We hypothesized that addition of gadolinium-based material to 3D-constructive interference in steady-state would improve characterization compression.Retrospective review high-resolution MRIs was performed patients without prior microvascular decompression....
The relay of visual information converging in the lateral geniculate nucleus (LGN) en route to cortex is modulated by projections from brainstem nuclei. release serotonin, one mediator these effects, has been shown act at a presynaptic site inhibit neurotransmitter retinogeniculate synapse, connection between retinal ganglion cells and thalamocortical neurons LGN. To understand how serotonergic inhibition synaptic transmission influences transfer this we examined EPSCs firing responses...
To determine if axial T2-weighted imaging can serve as screening tool for pediatric brain imaging.We retrospectively evaluated consecutive magnetic resonance (MRI) data of 161 children (74 girls) with a mean age 7.44 ± 5.71 years. Standard reference was the final report neuroradiology attendings. Three readers different levels experience were blinded clinical diagnoses and study indications. First, studied only sequence. Second, they all available anatomical functional MRI sequences...
<h3>BACKGROUND AND PURPOSE:</h3> Patients with trigeminal neuralgia often undergo rhizotomy via radiofrequency thermocoagulation or glycerol injection for treatment of symptoms. To date, radiologic changes in patients post-rhizotomy have not been described, to our knowledge. The aim this study was evaluate after characterize on 3D high-resolution MR imaging. <h3>MATERIALS METHODS:</h3> A retrospective review protocol studies performed 26 compared 54 treatment-naïve subjects neuralgia....
Intraventricular hemorrhage and posthemorrhagic hydrocephalus continue to be common complications in very low-birth-weight premature infants, often requiring ventricular shunting for cerebrospinal fluid diversion. We report on two infants with that developed a secondary encephalocele after ventriculosubgaleal shunting. Encephaloceles can act as source of seizure activity result various additional including meningitis, abscess formation, infarction herniated brain parenchyma. With continued...