A5027056106- Viral Infections and Immunology Research
- Cardiovascular Disease and Adiposity
- Atherosclerosis and Cardiovascular Diseases
- Cardiac Fibrosis and Remodeling
- Wound Healing and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Acute Myocardial Infarction Research
- Cardiovascular Effects of Exercise
- Advanced Glycation End Products research
- Burn Injury Management and Outcomes
- Clusterin in disease pathology
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Signaling Pathways in Disease
- Nitric Oxide and Endothelin Effects
- Cardiac Ischemia and Reperfusion
- Protein Kinase Regulation and GTPase Signaling
- Infective Endocarditis Diagnosis and Management
- Cardiac electrophysiology and arrhythmias
- Blood Coagulation and Thrombosis Mechanisms
- Folate and B Vitamins Research
- Cell Adhesion Molecules Research
- Lipoproteins and Cardiovascular Health
- Complement system in diseases
Amsterdam University Medical Centers
2018-2024
Vrije Universiteit Amsterdam
2009-2024
Amsterdam Neuroscience
2024
Amsterdam UMC Location Vrije Universiteit Amsterdam
2013-2022
University of Amsterdam
2022
Amsterdam UMC Location University of Amsterdam
2021
Indian Council of Agricultural Research
2004-2014
Maastricht University
2006
Sanquin
2006
Genmab (Netherlands)
2006
Monocytes are critical mediators of healing following acute myocardial infarction (AMI), making them an interesting target to improve repair. The purpose this study was a gain insight into the source and recruitment monocytes AMI in humans. Post-mortem tissue specimens myocardium, spleen bone marrow were collected from 28 patients who died at different time points after AMI. Twelve other causes served as controls. presence localization (CD14+ cells), their CD14+CD16– CD14+CD16+ subsets,...
Pro-coagulant and pro-inflammatory intramyocardial (micro)vasculature plays an important role in acute myocardial infarction (AMI). Currently, inhibition of serine protease dipeptidyl peptidase 4 (DPP4) receives a lot interest as anti-hyperglycemic therapy type 2 diabetes patients. However, DPP4 also possesses anti-thrombotic properties may behave immobilized anti-coagulant on endothelial cells. Here, we studied the expression activity human relation to prothrombogenic phenotype. Using...
T cell autoreactivity is a hallmark of autoimmune diseases but can also benefit self-maintenance and foster tissue repair. Herein, we investigated whether heart-specific cells exert salutary or detrimental effects in the context myocardial infarction (MI), leading cause death worldwide. After screening more than 150 class-II-restricted epitopes, found that myosin heavy chain alpha (MYHCA) was dominant cardiac antigen triggering post-MI CD4+ activation mice. Transferred MYHCA614-629-specific...
<b>Background/Aims:</b> Recent studies indicate the presence of reactive oxygen species (ROS) producing homologues enzymatic subunit (Nox2) phagocytic NADPH oxidase in non-phagocytic cells. Interestingly, these cells, ROS produced by Nox2 homologue(s) was shown to play a role various regulatory processes, including cell death, proliferation, and aging. The purpose this study investigate whether human cardiomyocytes express Nox2. <b>Methods:</b> expression studied using western blot...
Adipose-derived stromal cells (ASC) are promising candidates for cell therapy, example to treat myocardial infarction. Commonly, fetal bovine serum (FBS) is used in ASC culturing. However, FBS has several disadvantages. Its effects differ between batches and, when applied clinically, transmission of pathogens and antibody development against possible. In this study, we investigated whether can be substituted by human platelet lysate (PL) culture, without affecting functional capacities...
Apoptosis of endothelial cells related to homocysteine (Hcy) has been reported in several studies. In this study, we evaluated whether reactive oxygen species (ROS)-producing signaling pathways contribute Hcy-induced apoptosis induction, with specific emphasis on NADPH oxidases. Human umbilical vein were incubated 0.01–2.5 mM Hcy. We determined the effect Hcy caspase-3 activity, annexin V positivity, intracellular NOX1, NOX2, NOX4, and p47phox expression localization, nuclear nitrotyrosine...
Monocytes are involved in adverse left ventricular (LV) remodelling following myocardial infarction (MI). To provide therapeutic opportunities we aimed to identify gene transcripts monocytes that relate post-MI healing and evaluated intervention with the observed activity a rat MI model. In 51 patients treated by primary percutaneous coronary (PCI), change LV end-diastolic volume index (EDVi) from baseline 4-month follow-up was assessed using cardiovascular magnetic resonance imaging (CMR)....
Journal Article Acute Inflammation is Persistent Locally in Burn Wounds: A Pivotal Role for Complement and C-Reactive Protein Get access Franklin van de Goot, MD, MD *Department of Pathology, VU Medical Center, Amsterdam, The Netherlands§Department Dutch Forensic Institute, Rijswijk, Netherlands Search other works by this author on: Oxford Academic Google Scholar Paul A.J. Krijnen, PhD, PhD Netherlands‖ICaR-VU, **Address correspondence to Department Netherlands. Mark P.V. Begieneman, BSc,...
Eur J Clin Invest 2010; 40 (10): 893–902 Abstract Background Clusterin (Apolipoprotein J), a plasma protein with cytoprotective and complement‐inhibiting activities, localizes in the infarcted heart during myocardial infarction (MI). Recently, we have shown protective effect of exogenous clusterin vitro on ischaemically challenged cardiomyocytes independent complement. We therefore hypothesized that intravenous administration would reduce damage. Methods Wistar rats undergoing experimental...
We examined the relationship between clusterin and activated complement in human heart infarction evaluated effect of this protein on ischemic rat neonatal cardiomyoblasts (H9c2) isolated adult ventricular cardiomyocytes as vitro models acute myocardial infarction. Clusterin protects cells by inhibiting colocalizes with jeopardized after The distribution factor C3d was infarcted heart. also analyzed expression H9c2 cells. binding endogenous purified flow cytometry. Furthermore, viability...
Hyperhomocysteinaemia (HHC) is thought to be a risk factor for cardiovascular disease including heart failure. While numerous studies have analyzed the role of homocysteine (Hcy) in vasculature, only few investigated Hcy heart. Therefore we effects on isolated cardiomyocytes. H9c2 cells (rat cardiomyoblast cells) and adult rat cardiomyocytes were incubated with cell viability. Furthermore, determined intracellular mediators related viability cardiomyocytes, namely NOX2, reactive oxygen...
In a previous study, the authors found persistent presence of acute inflammation markers such as C-reactive protein and complement factors locally in burn wounds. This persistence may not only delay local wound healing but also have systemic effect, for instance on heart. Here, effects C1 esterase inhibitor (C1inh), an activation, progression heart were analyzed rats. Dorsal full-thickness wounds (2 × 4 cm) induced female Wistar rats (n = 14). The divided into two groups 7): control group...
Abstract Background Diabetes mellitus (DM) induces cardiac and cerebral microvascular dysfunction via increased glycation, oxidative stress endothelial activation. Liraglutide, a glucagon‐like peptide‐1 analogue, inhibited NOX2 adhesion molecules in isolated cells. Here, we have studied how Liraglutide affects advanced NOX expression inflammation of the cardiac, renal microvasculature diabetic rats. Methods DM was induced Sprague–Dawley rats ( n = 15) intraperitoneal streptozotocin (STZ)...
Reperfusion of ischaemic myocardium after acute myocardial infarction (AMI) can induce ischaemia/reperfusion (I/R) injury, as a result local activation the complement system. C reactive protein (CRP) is involved in this activation. This study analysed potential role IgM infarcted human myocardium.Immunochemical analysis was carried out on heart specimens from 59 patients who died AMI. Serial slides frozen tissue site were stained for IgM, factors C3d and C5b-9 (membrane attack complex),...
Advanced glycation end products (AGEs), such as N(epsilon)-(carboxymethyl)lysine (CML), are implicated in vascular disease. We previously reported increased CML accumulation small intramyocardial blood vessels diabetes patients. Diabetes patients have an risk for acute myocardial infarction (AMI). Here, we examined a putative relationship between and AMI.Heart tissue was stained CML, myeloperoxidase, E-selectin AMI (n=26), myocarditis (n=17), control (n=15). In patients, depositions were...