A5069684481- Photochemistry and Electron Transfer Studies
- Quantum Dots Synthesis And Properties
- Chalcogenide Semiconductor Thin Films
- Molecular Sensors and Ion Detection
- Alcoholism and Thiamine Deficiency
- Analytical Chemistry and Sensors
- Free Radicals and Antioxidants
- Nitric Oxide and Endothelin Effects
- Advanced biosensing and bioanalysis techniques
- Photosynthetic Processes and Mechanisms
- Protein Interaction Studies and Fluorescence Analysis
- Luminescence and Fluorescent Materials
- Photochromic and Fluorescence Chemistry
- Mass Spectrometry Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Electron Spin Resonance Studies
- Photoreceptor and optogenetics research
- Hemoglobin structure and function
- Heme Oxygenase-1 and Carbon Monoxide
- Gold and Silver Nanoparticles Synthesis and Applications
- Molecular spectroscopy and chirality
- Spectroscopy and Quantum Chemical Studies
- Lanthanide and Transition Metal Complexes
- Nanocluster Synthesis and Applications
- Sulfur Compounds in Biology
International Sakharov Environmental Institute
2023-2024
Belarusian State University
2023-2024
Yanka Kupala State University of Grodno
2010-2023
University of Virginia
2014-2015
Kampala University
2008
Russian Academy of Sciences
2007
National Academy of Sciences of Belarus
2007
Institute of Cytology
2007
Indiana University – Purdue University Indianapolis
2007
Université de Reims Champagne-Ardenne
2004-2006
The effect of solvent viscosity on thioflavin T (ThT) fluorescent properties is analyzed to understand the molecular mechanisms characteristic increase in ThT fluorescence intensity accompanying its incorporation into amyloid-like fibrils. To this end, dependencies quantum yield and lifetime temperature glycerol content water−glycerol mixtures are studied. It has been found that typical for specific class fluorophores known as rotors. established low solvents with caused by nonradiative...
Quantum-chemical calculations of the Thioflavin T (ThT) molecule in ground S0 and first excited singlet S1 states were carried out. It has been established that ThT state a noticeable nonplanar conformation: torsion angle φ between benzthiazole dimethylaminobenzene rings found to be ∼37°. The energy barriers intramolecular rotation appearing at = 0 90° are quite low: semiempirical AM1 PM3 methods predict values ∼700 cm-1 ab initio ∼1000−2000 cm-1. INDO/S vertical transitions S1(abs) have...
We demonstrate that the nanoscale assemblies of water-solubilized, oppositely charged CdSe/ZnS core/shell quantum dots and nanogold particles make it possible to develop FRET-based sensors with a donor quenching efficiency close 100%. The cw time-resolved photoluminescence analysis nanoassemblies different donor-to-acceptor ratios demonstrates prevalence 1:1 complexes. Experimental data perfectly fit mathematical simulation based on long-range FRET static model assembling nanoparticles....
The increase in the solvent polarity induces a significant shift of long-wavelength absorption band thioflavin T (ThT) to shorter wavelengths. This is due fact that positive charge ThT molecule (Z = +1e) unequally and very differently distributed between benzthiazole aminobenzene rings ground excited states. Therefore, state stabilized by orientational interactions polar dipoles with positively charged fragments, whereas configuration solvation shell Franck−Condon likely far from being...
Here we provide first direct experimental results about photoinduced TICT-state formation for Thioflavin T (ThT). In this work, femtosecond transient absorption spectra dynamics ThT, dissolved in low-viscosity solvents (water, ethanol, 2-propanol, butanol) was investigated. It found that decay lifetime of fluorescent LE-state ThT low-viscous does not exceed 12 ps, and its value correlates well with rising time the band at 470 nm. indicates initially formed upon photoexcitation is quite...
It has been established earlier that fluorescence quantum yield of thioflavin T (ThT)—a probe widely used for amyloid fibrils detection—is viscosity-dependent, and photophysical properties ThT can be well-described by the fluorescent molecular rotor model, which associates twisted internal charge transfer (TICT) reaction with main nonradiative decay process in excited state dye. Solutions a range polar solvents were studied using steady-state sub-picosecond transient absorption spectroscopy...
Highly ordered quasi-nanowires from fluorescent semiconductor CdSe/ZnS spherical (quantum dots) or rod-like rods) nanoparticles were produced using DNA as a template. Positively charged fixed along the negatively backbone by electrostatic interaction. After incubation of solution and at different stoichiometric ratios complexes applied to hydrophobic surface stretched molecular combing technique. Here, we demonstrate that patterns with desirable morphology properties can be formed varying...
S -Nitrosoglutathione is an endogenous airway smooth muscle relaxant. Increased -nitrosoglutathione breakdown occurs in some asthma patients. We asked whether patients with increased catabolism of this molecule had clinical features that distinguished them from other measured reductase expression and activity bronchoscopy samples taken 66 subjects the Severe Asthma Research Program. also analysed phenotype genotype data program as a whole. Airway was (p=0.032). However, only subpopulation...
We prepared for the first time photoactive colloidal nanospheres consisting of hydrophobic CdSe/ZnS core–shell quantum dots encapsulated in an amphiphilic PMAT polymeric shell containing photochromic diarylethene DAE1 with stoichiometry ∼6 dye molecules per single dot. The UV-induced photoisomerization results partial quenching dot emission due to FRET from photoisomer its absorption band spectrally overlapped emission. modulation is reversible under cyclic UV–vis-induced open–closed DAE1.
Context: Exogenous nitrogen oxides must be made bioavailable to sustain normal physiology because nitric oxide synthase (NOS) deficient mice are viable. In the stomach, S-nitrosoglutathione (GSNO) is formed from ingested nitrite and high levels of airway glutathione (GSH) that cleared swallowed. However, gastric GSNO may broken down by nutrients like ascorbic acid (AA) before it absorbed.Objective: To study effect AA on formation stability.Materials methods: GSH were reacted with or without...
Abstract Thioflavin T (ThT) is a viscosity‐sensitive fluorescent dye and its emission intensity undergoes significant enhancement upon binding to DNA or amyloid fibrils. This fluorescence light‐up feature has been attributed earlier restriction of structural rearrangements in the excited state that are coupled an intramolecular charge transfer (ICT) reaction. In this work TDDFT (using B3LYP CAM‐B3LYP functionals) SA‐2‐CASSCF calculations were carried out obtain relaxed excited‐state...
S-Nitroso compounds have received much attention in biological research. In addition to their role as nitric oxide donors, there is growing evidence that these are involved signaling processes systems. Determination of S-nitrosylated proteins great importance for fundamental research and medical applications. The most common method assay S-nitroso chemically or photochemically reduce SNO functional groups release oxide, which then entrained an inert gas stream detected, usually through...