A5089292947- CRISPR and Genetic Engineering
- Genetics, Aging, and Longevity in Model Organisms
- Evolution and Genetic Dynamics
- Chromosomal and Genetic Variations
- Insect Resistance and Genetics
University of Waterloo
2019-2020
Abstract Using CRISPR/Cas9 to simultaneously induce mutations in two or more target genes, commonly referred as multiplexing, may result chromosomal rearrangements such inversions translocations. While this be undesirable some contexts, the ability recover targeted specific sites genome is potentially a powerful tool. Before developing tools, however, it first important measure frequency with which chromosome are induced by multiplexing. To do this, we have developed self-selecting screening...
We have developed a CRISPR/Cas9 based method for isolating randomly induced recessive lethal mutations in gene of interest (GOI) by selection within the F1 progeny single genetic cross. Our takes advantage ability to overexpress GOI using mediated activation expression. In essence, screening strategy is upon idea that if overexpression wild type allele can generate phenotype, then newly loss-of-function will lack this phenotype. As proof-of-principle, we used select EMS Drosophila hindsight...
Abstract We have developed a CRISPR/Cas9 based method for isolating randomly induced recessive lethal mutations in gene of interest (GOI) by selection within the F1 progeny single genetic cross. Our takes advantage ability to overexpress GOI using mediated activation expression. In essence, screening strategy is upon idea that if overexpression wild type allele can generate phenotype, then newly loss-of-function will lack this phenotype. As proof-of-principle, we used select EMS Drosophila...