A5028308415- Genetics, Aging, and Longevity in Model Organisms
- Metal Extraction and Bioleaching
- CRISPR and Genetic Engineering
- Mine drainage and remediation techniques
- Animal Behavior and Reproduction
- Mining and Resource Management
- Evolution and Genetic Dynamics
- Insect and Arachnid Ecology and Behavior
- Minerals Flotation and Separation Techniques
- Insect Resistance and Genetics
- Neurobiology and Insect Physiology Research
University of Waterloo
2019-2024
University of Toronto
2021
Thermal recovery technologies for in-situ bitumen extraction can result in the heating of surrounding aquifers, potentially mobilizing arsenic naturally present sediments to groundwater. The relative toxicity dissolved is related its speciation, with As(V) being less toxic than As(III). Microorganisms have various mechanisms detoxification and metabolism, which include genes efflux, methylation, reduction/oxidation As(V)/As(III). We characterized microbial communities along two aquifer...
ABSTRACT The oxidation of sulfide-bearing mine tailings catalysed by acidophilic iron and sulfur-oxidizing bacteria releases toxic metals other contaminants into soil groundwater as acid drainage. Understanding the environmental variables that control community structure metabolic activity endogenous microbes living in (especially abiotic stressors low pH high dissolved metal content) is crucial to developing sustainable bioremediation strategies. We determined microbial composition along...
ABSTRACT The oxidation of sulfide-bearing mine tailings catalyzed by acidophilic iron and sulfur-oxidizing bacteria releases toxic metals other contaminants into soil groundwater as acid drainage. Understanding the environmental variables that control community structure metabolic activity microbes indigenous to (especially abiotic stressors low pH high dissolved metal content) is crucial developing sustainable bioremediation strategies. We determined microbial composition along two...
We have developed a CRISPR/Cas9 based method for isolating randomly induced recessive lethal mutations in gene of interest (GOI) by selection within the F1 progeny single genetic cross. Our takes advantage ability to overexpress GOI using mediated activation expression. In essence, screening strategy is upon idea that if overexpression wild type allele can generate phenotype, then newly loss-of-function will lack this phenotype. As proof-of-principle, we used select EMS Drosophila hindsight...
Abstract We have developed a CRISPR/Cas9 based method for isolating randomly induced recessive lethal mutations in gene of interest (GOI) by selection within the F1 progeny single genetic cross. Our takes advantage ability to overexpress GOI using mediated activation expression. In essence, screening strategy is upon idea that if overexpression wild type allele can generate phenotype, then newly loss-of-function will lack this phenotype. As proof-of-principle, we used select EMS Drosophila...