A5047671159- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Microbial Metabolites in Food Biotechnology
- Enzyme Catalysis and Immobilization
- Studies on Chitinases and Chitosanases
- Polysaccharides and Plant Cell Walls
- Infant Nutrition and Health
- Microbial Metabolic Engineering and Bioproduction
- Glycosylation and Glycoproteins Research
- Digestive system and related health
- Phytase and its Applications
- Protein Hydrolysis and Bioactive Peptides
- Carbohydrate Chemistry and Synthesis
- Seaweed-derived Bioactive Compounds
- Food composition and properties
- Diet, Metabolism, and Disease
- Effects and risks of endocrine disrupting chemicals
- biodegradable polymer synthesis and properties
- Probiotics and Fermented Foods
- Legume Nitrogen Fixing Symbiosis
- Plant biochemistry and biosynthesis
- Microplastics and Plastic Pollution
- Enzyme Structure and Function
- Pineapple and bromelain studies
- Viral Infections and Vectors
China Agricultural University
2016-2025
China National Research Institute of Food and Fermentation
2025
Southeast University
2023
Van Andel Institute
2017
Heidelberg University
1993
Abstract Alginate, a group of polyuronic saccharides, has been widely used in both pharmaceutical and food industries due to its unique physicochemical properties as well beneficial health effects. However, the potential applications alginate are restricted because low water solubility high solution viscosity when significant concentrations needed, particularly products. Alginate oligosaccharides (AOS), oligomers containing 2 25 monomers, can be obtained via hydrolysis glycosidic bonds,...
An alginate lyase (FsAly7) from
N-acetyl-β-D-glucosamine (GlcNAc) is widely used as a valuable pharmacological agent and functional food additive. The traditional chemical process for GlcNAc production has some problems such high cost, low yield, acidic pollution. Hence, to identify novel chitinase that suitable bioconversion of chitin great value. A gene (PbChi74) from Paenibacillus barengoltzii was cloned heterologously expressed in Escherichia coli an intracellular soluble protein. open reading frame (ORF) 2,163 bp...
Difucosyllactose (DFL) is an important component of human milk oligosaccharides (HMOs) and has significant benefits for the growth development infants. So far, a few microbial cell factories have been constructed production DFL, which still problems low high cost. Herein, high-level de novo pathway DFL-producing strain was by multistep optimization strategies in Escherichia coli BL21star(DE3). We first efficiently synthesized intermediate 2′-fucosyllactose (2′-FL) E. BL21star(DE3) advisable...
Production, purification, and characterization of a novel β-1,3-1,4-glucanase (lichenase) from thermophilic Rhizomucor miehei CAU432 were investigated. High-level extracellular production 6230 U/mL was obtained when oat flour (3%, w/v) used as carbon source at 50 °C. The crude enzyme purified to homogeneity with specific activity 28818 U/mg. molecular weight estimated be 35.4 kDa 33.7 by SDS-PAGE gel filtration, respectively. optimal pH temperature the 5.5 60 °C, K(m) values for barley...
Hantavirus nucleocapsid protein has recently been identified as a major antigen inducing an early and long-lasting humoral immune response in patients with hemorrhagic fever renal syndrome. A mu-capture enzyme-linked immunosorbent assay utilizing recombinant proteins of strains Hantaan 76-118 (Hantaan serotype) CG 18-20 (Puumala diagnostic antigens specific monoclonal antibodies the detection system developed. Histidine-tailed were expressed Escherichia coli purified single step by affinity...
The purification and characterization of a novel extracellular beta-glucosidase from Paecilomyces thermophila J18 was studied. purified to 105-fold apparent homogeneity with recovery yield 21.7% by DEAE 52 Sephacryl S-200 chromatographies. Its molecular masses were 116 197 kDa when detected SDS-PAGE gel filtration, respectively. It homodimeric glycoprotein carbohydrate content 82.3%. enzyme exhibited an optimal activity at 75 degrees C pH 6.2. stable up 65 in the range 5.0-8.5. broad...
The purification and characterization of a novel extracellular β-1,3-1,4-glucanase from the thermophilic fungus Paecilomyces thermophila J18 were studied. strain produced maximum level β-glucanase (135.6 U mL−1) when grown in medium containing corncob (5%, w/v) at 50 °C for 4 days. crude enzyme solution was purified by 122.5-fold with an apparent homogeneity recovery yield 8.9%. showed as single protein band on SDS-PAGE molecular mass 38.6 kDa. masses 34.6 kDa 31692.9 Da detected gel...
An endo-1,4-β-mannanase gene (RmMan5A) was cloned from the thermophilic fungus Rhizomucor miehei for first time and expressed in Escherichia coli . The had an open reading frame of 1330 bp encoding 378 amino acids contained four introns. It displayed highest acid sequence identity (42%) with endo-1,4-β-mannanases glycoside hydrolase family 5. purified enzyme a monomer 43 kDa. RmMan5A maximum activity at 55 °C optimal pH 7.0. thermostable up to alkali-tolerant, displaying excellent stability...
β-1,3-Glucan is one of the most abundant polysaccharides in fungi.