A5011696144- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Microbial Metabolites in Food Biotechnology
- Enzyme Catalysis and Immobilization
- Studies on Chitinases and Chitosanases
- Protein Hydrolysis and Bioactive Peptides
- Polysaccharides and Plant Cell Walls
- Phytase and its Applications
- Microbial Metabolic Engineering and Bioproduction
- Infant Nutrition and Health
- Glycosylation and Glycoproteins Research
- Digestive system and related health
- Polyamine Metabolism and Applications
- Seaweed-derived Bioactive Compounds
- Gut microbiota and health
- Probiotics and Fermented Foods
- Food composition and properties
- Diet, Metabolism, and Disease
- Carbohydrate Chemistry and Synthesis
- Polysaccharides Composition and Applications
- Pineapple and bromelain studies
- Natural product bioactivities and synthesis
- Biopolymer Synthesis and Applications
- Insect Utilization and Effects
- Effects and risks of endocrine disrupting chemicals
China Agricultural University
2016-2025
Nanjing University of Finance and Economics
2022-2024
Chang'an University
2012
Abstract Alginate, a group of polyuronic saccharides, has been widely used in both pharmaceutical and food industries due to its unique physicochemical properties as well beneficial health effects. However, the potential applications alginate are restricted because low water solubility high solution viscosity when significant concentrations needed, particularly products. Alginate oligosaccharides (AOS), oligomers containing 2 25 monomers, can be obtained via hydrolysis glycosidic bonds,...
A novel fungal gene encoding the Rhizomucor miehei l-asparaginase (RmAsnase) was cloned and expressed in Escherichia coli. Its deduced amino acid sequence shared only 57% identity with sequences of other reported l-asparaginases. The purified homodimer had a molecular mass 133.7 kDa, high specific activity 1,985 U/mg, very low glutaminase activity. RmAsnase optimally active at pH 7.0 45°C stable this temperature for 30 min. final level acrylamide biscuits bread decreased by about 81.6%...
An alginate lyase (FsAly7) from
N-acetyl-β-D-glucosamine (GlcNAc) is widely used as a valuable pharmacological agent and functional food additive. The traditional chemical process for GlcNAc production has some problems such high cost, low yield, acidic pollution. Hence, to identify novel chitinase that suitable bioconversion of chitin great value. A gene (PbChi74) from Paenibacillus barengoltzii was cloned heterologously expressed in Escherichia coli an intracellular soluble protein. open reading frame (ORF) 2,163 bp...
The feasibility of a protease Pseudomonas aeruginosa (PaproA) to prepare angiotensin I-converting enzyme (ACE) inhibitory peptides from wheat gluten was evaluated according physiochemical and antihypertensive performances. hydrolyzed by Alcalase plus PaproA (WGH_APae) produce small fragments (<1 kDa) particularly preferred the superior protein recovery rate, degree hydrolysis, ACE potential, stability against gastrointestinal digestion. Among resultant fractions WGH_APae, lower IC50 value...
Difucosyllactose (DFL) is an important component of human milk oligosaccharides (HMOs) and has significant benefits for the growth development infants. So far, a few microbial cell factories have been constructed production DFL, which still problems low high cost. Herein, high-level de novo pathway DFL-producing strain was by multistep optimization strategies in Escherichia coli BL21star(DE3). We first efficiently synthesized intermediate 2′-fucosyllactose (2′-FL) E. BL21star(DE3) advisable...
Production, purification, and characterization of a novel β-1,3-1,4-glucanase (lichenase) from thermophilic Rhizomucor miehei CAU432 were investigated. High-level extracellular production 6230 U/mL was obtained when oat flour (3%, w/v) used as carbon source at 50 °C. The crude enzyme purified to homogeneity with specific activity 28818 U/mg. molecular weight estimated be 35.4 kDa 33.7 by SDS-PAGE gel filtration, respectively. optimal pH temperature the 5.5 60 °C, K(m) values for barley...