A5020088375- Plant nutrient uptake and metabolism
- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Agronomic Practices and Intercropping Systems
- Data Analysis with R
- Soil Carbon and Nitrogen Dynamics
- Nematode management and characterization studies
- Soil Moisture and Remote Sensing
- Plant Parasitism and Resistance
- Plant Disease Resistance and Genetics
- Plant Reproductive Biology
- Soybean genetics and cultivation
Australian National University
2015-2022
Initiation of symbiotic nodules in legumes requires cytokinin signaling, but its mechanism action is largely unknown. Here, we tested whether the failure to initiate Medicago truncatula perception mutant cre1 (cytokinin response1) due altered ability regulate auxin transport, accumulation, and induction flavonoids. We found that mutant, rhizobia cannot locally alter acro- basipetal transport during nodule initiation these mutants show reduced (indole-3-acetic acid) accumulation responses...
Summary Root system architecture (RSA) influences the effectiveness of resources acquisition from soils but genetic networks that control RSA remain largely unclear. We used rhizoboxes, X‐ray computed tomography, grafting, auxin transport measurements and hormone quantification to demonstrate Arabidopsis Medicago CEP (C‐TERMINALLY ENCODED PEPTIDE)‐CEP RECEPTOR signalling controls RSA, gravitropic set‐point angle (GSA) lateral roots (LRs), levels transport. showed soil‐grown receptor mutants...
The presence of nitrogen inhibits legume nodule formation, but the mechanism this inhibition is poorly understood. We found that 2.5 mM nitrate and above significantly inhibited initiation not root hair curling in Medicago trunatula. analyzed protein abundance M. truncatula roots after treatment with either 0 or absence its symbiont Sinorhizobium meliloti 1, 2 5 days following inoculation. Two-dimensional gel electrophoresis combined mass spectrometry was used to identify 106 differentially...
Abstract Root phenotyping describes methods for measuring root properties, or traits. While can be challenging, it is advancing quickly. In order the field to move forward, essential understand current state and challenges of phenotyping, as well pressing needs biology community. this letter, we present discuss results a survey that was created disseminated by members Graduate Student Postdoc Ambassador Program at 11th symposium International Society Research. This aimed (1) provide an...
Legumes enter into a symbiotic relationship with nitrogen-fixing rhizobia, leading to nodule development. Two main types of nodules have been widely studied, indeterminate and determinate, which differ in the location first cell division root cortex, persistency meristem. Here we compared control auxin transport, content response during early stages determinate development model legumes Medicago truncatula Lotus japonicus, respectively, investigate whether differences transport could explain...
Actinorhizal nodules are structurally different from legume and show a greater similarity to lateral roots. Because of the important role auxins in root nodule formation, auxin profiles were examined roots actinorhizal species Datisca glomerata model Medicago truncatula. The response both was analyzed transgenic systems expressing beta-glucuronidase gene under control synthetic auxin-responsive promoter DR5. effects two on development compared for species. present at highest levels...
The development of root nodules leads to an increased auxin response in early nodule primordia, which is mediated by changes acropetal transport some legumes. Here, we investigated the role basipetal during nodulation. Rhizobia inoculation significantly both Medicago truncatula and Lotus japonicus. In M. truncatula, this increase was dependent on functional Nod factor signalling through NFP, NIN, NSP2, as well ethylene SKL. To test whether required for nodulation, examined a loss-of-function...
Auxins represent a major group of phytohormones controlling plant development. The spatio-temporal regulation auxin gradients is essential for the initiation, growth and correct development organs. Because auxins their metabolites occur at trace levels in tissue, experiments requiring identification plus selective specific quantification can be most conveniently achieved using mass spectrometry (MS) associated chromatographic methods. With advent appropriate liquid-based ionisation...