A5015593712- DNA Repair Mechanisms
- RNA modifications and cancer
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Genetic and Mutation Studies
- Bacterial Genetics and Biotechnology
- Fermentation and Sensory Analysis
- Horticultural and Viticultural Research
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- MicroRNA in disease regulation
- Glycosylation and Glycoproteins Research
- Chromosomal and Genetic Variations
- Molecular Biology Techniques and Applications
- Phytochemicals and Antioxidant Activities
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Peptidase Inhibition and Analysis
- 3D Printing in Biomedical Research
- Gene expression and cancer classification
- ATP Synthase and ATPases Research
- Genetics, Aging, and Longevity in Model Organisms
- Mitochondrial Function and Pathology
- Signaling Pathways in Disease
- Genomics and Chromatin Dynamics
- Cancer Mechanisms and Therapy
Pfizer (United States)
2021-2023
Indiana University Bloomington
2017-2021
PIF1 family helicases are conserved from bacteria to man. With the exception of well-studied yeast (e.g., ScPif1 and ScRrm3), however, very little is known about how these enzymes help maintain genome stability. Indeed, we lack a basic understanding protein domains found N- C-terminal characteristic central helicase domain in proteins. Here, using chimeric constructs, show that ScRrm3 interchangeable N-terminus important for its function vivo. This suggests evolved functional modules fused...
In Saccharomyces cerevisiae, the Pif1 helicase functions in both nuclear and mitochondrial DNA replication repair processes, preferentially unwinding RNA:DNA hybrids resolving G-quadruplex structures. We sought to determine how various activities of are regulated vivo. Here, we report lysine acetylation demonstrate that it influences Pif1's cellular roles core biochemical activities. Using overexpression toxicity assays, determined acetyltransferase NuA4 deacetylase Rpd3 primarily...
Pif1 family helicases represent a highly conserved class of enzymes involved in multiple aspects genome maintenance. Many are multi-domain proteins, but the functions their non-helicase domains poorly understood. Here, we characterized how N-terminal domain (NTD) Saccharomyces cerevisiae helicase affects its both vivo and vitro. Removal NTD alleviated toxicity associated with overexpression yeast. Biochemically, N-terminally truncated (Pif1ΔN) retained vitro DNA binding, unwinding,...
Abstract PIF1 family helicases represent a highly conserved class of enzymes involved in multiple aspects genome maintenance. Many helicase are multi-domain proteins, but the functions their non-helicase domains poorly understood. Here, we characterized how N-terminal domain (NTD) Saccharomyces cerevisiae Pif1 affects its both vivo and vitro . Removal NTD alleviated toxicity associated with over-expression yeast. Biochemically, N-terminally truncated (Pif1ΔN) retained DNA binding, unwinding,...
Abstract In the beverage fermentation industry, especially at craft or micro level, there is a movement to incorporate as many local ingredients possible both capture terroir and stimulate economies. case of beer, this has traditionally only encompassed locally sourced barley, hops, other agricultural adjuncts. The identification use novel yeasts in brewing lags behind. We sought bridge gap by bio-prospecting for wild yeasts, with focus on American Midwest. isolated 284 different strains...
Glycosylation is a common post-translation modification present on majority of eukaryotic proteins. Therefore, it dependent the host cell line and affected by culture purification process. Additionally, glycosylation therapeutic biologics known to have profound impact binding downstream effector functions. Hence, increasingly considered critical quality attribute (CQA) biopharmaceutical drugs has been showed affect safety efficacy these molecules. regulatory agencies adopted Quality Design...
The comparison of growth, whether it is between different strains or under growth conditions, a classic microbiological technique that can provide genetic, epigenetic, cell biological, and chemical biological information depending on how the assay used. When employing solid media, this limited by being largely qualitative low throughput. Collecting data in form curves, especially automated collection multi-well plates, circumvents these issues. However, curves themselves are subject to...
Petite integration frequency 1 (Pif1) is a 5′‐3′ helicase which plays significant role in maintaining the fidelity of nuclear and mitochondrial DNA. Pif1 preferentially unwinds RNA‐DNA hybrids during key metabolic processes such as DNA replication, repair telomere maintenance. Regulation activity by phosphorylation modulates this Our current work focused on understanding how lysine acetylation modifies protein's activity. Lysine proteins dynamic modification that regulated acetyltransferases...
ABSTRACT In S. cerevisiae , the Pif1 helicase functions to impact both nuclear and mitochondrial DNA replication repair processes. is a 5’-3’ helicase, which preferentially unwinds RNA-DNA hybrids resolves G-quadruplex structures. Further, regulation of by phosphorylation negatively impacts its interaction with telomerase during double strand break repair. Here, we report that in addition phosphorylation, also modified lysine acetylation, influences cellular core biochemical activities....
Abstract Glycosylation is a common post-translation modification present on majority of eukaryotic proteins. Therefore, it dependent the host cell line and affected by culture purification process. Additionally, glycosylation therapeutic biologics known to have profound impact binding downstream effector functions. Hence, increasingly considered critical quality attribute (CQA) biopharmaceutical drugs has been showed affect safety efficacy these molecules. regulatory agencies adopted Quality...
ABSTRACT PIF1 family helicases are conserved from bacteria to man. With the exception of well-studied yeast ( e.g ., ScPif1 and ScRrm3), however, very little is known about how these enzymes help maintain genome stability. Indeed, we lack a basic understanding protein domains found N- C-terminal characteristic central helicase domain in proteins. Here, using chimeric constructs, show that ScRrm3 interchangeable N-terminus important for its function vivo . This suggests evolved functional...