A5064435188- Microbial Fuel Cells and Bioremediation
- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Electrochemical sensors and biosensors
- Neonatal and Maternal Infections
- Electrochemical Analysis and Applications
- Metalloenzymes and iron-sulfur proteins
- Biosensors and Analytical Detection
- bioluminescence and chemiluminescence research
- Infections and bacterial resistance
- Antimicrobial Peptides and Activities
- Advanced battery technologies research
- Neuroscience and Neural Engineering
- CO2 Reduction Techniques and Catalysts
- Modular Robots and Swarm Intelligence
- Protist diversity and phylogeny
- ATP Synthase and ATPases Research
- Photosynthetic Processes and Mechanisms
- Gut microbiota and health
- Infective Endocarditis Diagnosis and Management
- Bacteriophages and microbial interactions
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Oral microbiology and periodontitis research
- Analytical Chemistry and Sensors
Rice University
2020-2023
Lawrence Berkeley National Laboratory
2016-2021
University of California, Berkeley
2019
Hebrew University of Jerusalem
2007-2014
Microbial electrochemical systems provide an environmentally-friendly means of energy conversion between chemical and electrical forms, with applications in wastewater treatment, bioelectronics, biosensing. However, a major challenge to further development, miniaturization, deployment bioelectronics biosensors is the limited thickness biofilms, necessitating large anodes achieve sufficient signal-to-noise ratios. Here we demonstrate method for embedding electroactive bacterium, Shewanella...
Highlights•Group A streptococcus (GAS) induces ER stress and asparagine production•Asparagine alters GAS gene expression increases bacterial multiplication•Expression of streptolysins causing is augmented in absence•Asparaginase inhibits bacteremiaSummarySuccessful infection depends on the ability pathogen to gain nutrients from host. The extracellular pathogenic bacterium group Streptococcus causes a vast array human diseases. By using quorum-sensing sil system as reporter, we found that,...
Some anaerobic bacteria use insoluble minerals as terminal electron acceptors and discovering the ways in which electrons move through membrane barrier to exterior acceptor forms an active field of research with implications for both bacterial physiology bioenergy. A previous study suggested that Shewanella oneidensis MR-1 utilizes a small, polar, redox molecule serves shuttle between acceptors, but itself has never been identified. Through isolation synthesis, we identify it ACNQ...
Genetic circuits that encode extracellular electron transfer (EET) pathways allow the intracellular state of Escherichia coli to be electronically monitored and controlled. However, relatively low flux flows through these pathways, limiting degree control by circuits. Since EET pathway is composed multiple multiheme cytochromes c (cyts c) from Shewanella oneidensis MR-1, we hypothesized lower expression levels cyt may explain this caused differences in maturation (ccm) machinery between two...
Current technologies are lacking in the area of deployable, situ monitoring complex chemicals environmental applications. Microorganisms metabolize various chemical compounds and can be engineered to analyte-specific making them naturally suited for robust sensing. However, current electrochemical microbial biosensors use large expensive electrochemistry equipment not suitable on-site, real-time analysis. Here we demonstrate a miniaturized, autonomous bioelectronic sensing system (BESSY)...
In the last two decades an increasing number of local outbreaks invasive group A streptococcus (GAS) infections including necrotizing fasciitis (NF) have been reported. We identified streptococcal invasion locus (sil) which is essential for virulence M14 strain JS95 isolated from NF patient. This contains six genes: silA/B and silD/E encoding two-component system (TCS) ABC transporter, respectively, homologous to corresponding entities in regulon Streptococcus pneumoniae involved genetic...
Group A streptococcus (GAS) causes a wide variety of human diseases, and at the same time, GAS can also circulate without producing symptoms, similar to its close commensal relative, group G (GGS). We previously identified, by transposon-tagged mutagenesis, streptococcal invasion locus (sil). sil is quorum-sensing regulated which activated autoinducer peptide SilCR through two-component system SilA-SilB. Here we characterize DNA promoter region necessary for SilA-mediated activation. This...
Microorganisms regulate the redox state of different biomolecules to precisely control biological processes. These processes can be modulated by electrochemically coupling intracellular an external electrode, but current approaches afford only limited and specificity. Here we describe specific electrochemical reduction in Escherichia coli through introduction a heterologous electron transfer pathway. E. expressing cymAmtrCAB from Shewanella oneidensis MR-1 consumed electrons directly cathode...
Abstract Microorganisms regulate the redox state of different biomolecules to precisely control biological processes. These processes can be modulated by electrochemically coupling intracellular an external electrode, but current approaches afford only limited and specificity. Here we describe specific electrochemical reduction in Escherichia coli through introduction a heterologous electron transfer pathway. E. expressing mtrCAB from Shewanella oneidensis MR-1 consumed electrons directly...
Electron transfer between proteins in microorganisms and solid materials underpins many aspects of bioelectrochemical energy research. My laboratory seeks to tailor both new microbes for specific applications. Here I will first describe how we have customized the industrial microbe Escherichia coli use biological fuel cells. By transplanting Mtr pathway Shewanella oneidensis MR-1 into coli, can confer upon these cells ability reduce metal ions, oxides, electrodes. These couple current...