Abstract Urease-producing bacteria (UPB) provide inorganic nitrogen for primary producers by hydrolyzing urea, and play an important role in marine cycle. However, there is still incomplete understanding of UPB their ecological functions the cultivation environment red macroalgae Gracilariopsis lemaneiformis . This study comprehensively analyzed diversity culturable explored effects on urea uptake G. A total 34 isolates belonging to four main bacterial phyla i.e. (Proteobacteria,...

Abstract Macroalgae host various symbionts on their surface, which play a critical role in growth and development processes. However, there is still incomplete understanding of this epiphytic bacteria-host algae interactions. This study comprehensively analysed variation the bacterial communities (EBC) composition red macroalga Gracilariopsis lemaneiformis at different geographic locations environmental factors (i.e., nitrogen phosphorus), shape EBC G. . The structure were characterized...

ABSTRACT Urea is an important source of nitrogen for many phytoplankton with the potential to stimulate harmful algal blooms, but molecular machinery underpinning urea uptake and assimilation by algae not fully understood. Urease (URE) commonly regarded as responsible enzyme, amidolyase (UAL), albeit known exist, has hardly been studied. Here, species distribution, expression patterns functional roles UAL are examined. We found a widespread occurrence across six major lineages, along...

ABSTRACT High CO 2 emissions originating from anthropogenic sources have resulted in ocean warming (OW), posing a severe threat to marine organisms and ecosystems. Recent evidence has shown that phytoplankton may acclimate adapt long‐term OW. Whether how diatoms—a functional group of contributes 40% primary production—can OW remains virtually unknown. The model diatom Phaeodactylum tricornutum was subjected thermal stress (25°C, compared the Control at 20°C) for 400 days (~400 generations),...

Chlamydomonas reinhardtii is a unicellular green alga that can grow heterotrophically by using acetate as carbon source. Carotenoids are natural pigments with biological activity and color, which have functions such antioxidant, anti-inflammatory, vision protection, etc., high commercial value prospects. We transformed the BKT genes from Phaffia rhodozyma (PrBKT) (CrBKT) via plasmid vector, screened out stable algal strains C18 P1. Under condition cell density of growth was not affected,...

N-acyl homoserine lactones (AHLs) are small, diffusible chemical signal molecules that serve as social interaction tools for bacteria, enabling them to synchronize their collective actions in a density-dependent manner through quorum sensing (QS). The QS activity from epiphytic bacteria of the red macroalgae Porphyra haitanensis, along with its involvement biofilm formation and regulation, remains unexplored prior scientific inquiries. Therefore, this study explores AHL produced by bacteria....

Background The green alga Chlamydomonas reinhardtii can grow photoautotrophically utilizing light and CO 2 , heterotrophically acetate. physiological biochemical responses of autotrophy heterotrophy are different in C. . However, there is no complete understanding the molecular physiology between heterotrophy. Therefore, we performed biochemical, transcriptome analysis Results cell growth characterization demonstrated that heterotrophic had enhanced rates, autotrophic accumulated more...

The knowledge graph embedding model aims to use low-dimensional real-valued vectors represent the entities and relations in triples, where operations such as link prediction triple classification can be performed based on these representations. However, existing models only consider structural of while ignoring semantic information triples. This paper proposes a learning framework combined with (KGSE). KGSE comprehensively considers is used supplement improve quality embedding. Specifically,...

Abstract Urea is a crucial nitrogen nutrient source for algae with the potential to stimulate harmful algal blooms, but molecular machinery underpinning urea uptake and assimilation by not fully understood. Urease (URE) commonly regarded as responsible enzyme, amidolyase (UAD) system, albeit known exist, has hardly been studied. Here, phylogenetic distribution, expression patterns, functional roles of UAD system are examined, which comprises subunits DUR1 , DUR2 DUR3 . We find widespread...

Aspect-oriented modeling can uncover potential design faults, yet most existing work fails to achieve both separation and composition in a natural succinct way. This study presents an aspect-oriented analysis approach with hierarchical Coloured Petri Nets (HCPN). HCPN has sub-models well-defined semantics combining set of submodels. These two characteristics are nicely integrated into aspect oriented modeling. Submodels used model aspects while the combination mechanism contributes weaving....

Abstract As a new computing architecture, edge has gradually penetrated into the smart grid and provide real time monitoring control. However, as type of node arranged in network, its own security protection become an incredibly challenging task. Security authentication is first line defence for protection. Due to fast light-weight characteristics, physical layer channel information access technology non-cryptographic packets potential identification method terminal under system, which...

Abstract Macroalgae host variety of symbionts on their surface which play critical role in growth and development processes. Nevertheless, the complete understanding this interaction epiphytic bacteria algae is still its infancy. This study comprehensively analyses bacterial communities composition red macroalga Gracilariopsis lemaneiformis environmental factors such as nitrogen phosphorus shape G. variation at different geographical location. The structure were characterized using high...

Abstract Urease-producing bacteria (UPB) provide inorganic nitrogen for primary producers by hydrolyzing urea, and play an important role in marine cycle. However, there is still incomplete understanding of UPB their ecological functions the cultivation environment red macroalgae Gracilariopsis lemaneiformis . This study comprehensively analyzed diversity culturable explored effects on urea uptake G. A total 34 isolates belonging to four main bacterial phyla i.e. (Proteobacteria,...

Enabling data sharing among smart grid power suppliers is a pressing challenge due to technical hurdles in verifying, storing, and synchronizing energy metering data. Access limitations are stringent for users, companies, researchers, demanding significant resources time permissions verification. This paper proposes blockchain-based architecture secure efficient of electric Further, we propose model based on evolutionary game theory. Based the Lyapunov stability theory, model’s stable...

<title>Abstract</title> <bold>Background </bold> <italic>Chlamydomonas reinhardtii </italic>can grow photoautotrophically and heterotrophically, is the typical model species for heterotrophy increasingly cultured in commercial cultivation. By observing their growth changes, transcriptomic analysis molecular regulation, we investigated mechanism of differences from photoautotrophy 12h (P12h) to (H12h). <bold>Results</bold> We observed changes cell numbers, OD750, Fv/Fm, total chlorophyll...