A5027245539- Environmental DNA in Biodiversity Studies
- Identification and Quantification in Food
- Fish Ecology and Management Studies
- Marine Ecology and Invasive Species
- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- Fish Biology and Ecology Studies
University of Minho
2020-2024
Aquatic Systems (United States)
2024
Research Network (United States)
2024
Over the last two decades, use of DNA barcodes has transformed our ability to identify and assess life on planet. Both strengths weaknesses method have been exemplified through thousands peer-reviewed scientific articles. Given novel sequencing approaches, currently capable generating millions reads at low cost, we reflect questions: What will future bring for barcoding? Will identification species using short, standardized fragments stand test time? We present reflected opinions early...
Abstract Biodiversity studies greatly benefit from molecular tools, such as DNA metabarcoding, which provides an effective identification tool in biomonitoring and conservation programmes. The accuracy of species‐level assignment, consequent taxonomic coverage, relies on comprehensive barcode reference libraries. role these libraries is to support species identification, but accidental errors the generation barcodes may compromise their accuracy. Here, we present R‐based application,...
The introduction of non-indigenous species (NIS) is one the major threats to integrity European coastal ecosystems. DNA-based assessments have been increasingly adopted for monitoring NIS. However, accuracy taxonomic assignments largely dependent on completion and reliability DNA barcode reference libraries. As such, we aimed compile audit a library marine invertebrate NIS occurring in Europe. To do so, compiled list using three databases: Alien Species Information Network (EASIN), System...
Environmental DNA (eDNA) metabarcoding is revolutionizing the study of aquatic ecosystems, enabling high-throughput analysis biodiversity with minimal disturbance. Despite its potential to support fisheries management, species identification and downstream reliability are hindered by lack standardization in fragment choice. This compares discrimination power three markers used marine fish eDNA —12S rRNA, 16S cytochrome oxidase subunit I (COI)— as well two amplicons for...
Ichthyoplankton monitoring is crucial for stock assessments, offering insights into spawning grounds, size, seasons, recruitment, and changes in regional ichthyofauna. This study evaluates the efficiency of multi-marker DNA metabarcoding using mitochondrial cytochrome c oxidase subunit I (COI), 12S rRNA 16S gene markers, comparison to morphology-based methods fish species identification ichthyoplankton samples. Two transects with four coastal distance categories were sampled along southern...
Over the last two decades, use of DNA barcodes has transformed our ability to identify and assess life on planet. Both strengths weaknesses method have been exemplified through thousands peer-reviewed scientific articles. In light novel sequencing approaches, currently capable generating millions reads at low cost, we reflect questions: what will future bring for barcoding? Will identification species using short, standardized fragments stand test time? We present reflected opinions early...
<title>Abstract</title> Ichthyoplankton monitoring is crucial for stock assessments, offering insights into spawning grounds, size, seasons, recruitment, and changes in regional ichthyofauna. This study evaluates the efficiency of multi-marker DNA metabarcoding using mitochondrial cytochrome c oxidase subunit I (COI), 12S rRNA 16S gene markers, comparison to morphology-based methods fish species identification ichthyoplankton samples. Two transects off southern coast Portugal were sampled at...
Environmental DNA (eDNA) metabarcoding is revolutionising the study of aquatic ecosystems, enabling high-throughput analysis biodiversity with minimal disturbance. Despite its potential to support fisheries management, species identification and downstream reliability are hindered by lack standardisation in fragment choice. This compares discrimination power three markers used marine fish (e)DNA (meta)barcoding – 12S rRNA, 16S rRNA cytochrome oxidase subunit I (COI) as well two amplicons for...