A5044607536- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Listeria monocytogenes in Food Safety
- Plant Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Probiotics and Fermented Foods
- Pharmaceutical and Antibiotic Environmental Impacts
- Food Safety and Hygiene
- Microbial infections and disease research
- Gut microbiota and health
- Ruminant Nutrition and Digestive Physiology
- Agriculture, Soil, Plant Science
- Microbial Inactivation Methods
- Biosensors and Analytical Detection
- Clostridium difficile and Clostridium perfringens research
- Animal Disease Management and Epidemiology
- Vibrio bacteria research studies
- Mycobacterium research and diagnosis
- Spectroscopy Techniques in Biomedical and Chemical Research
- Soil Carbon and Nitrogen Dynamics
- Essential Oils and Antimicrobial Activity
Agricultural Research Service
2016-2025
United States Department of Agriculture
2016-2025
Beltsville Agricultural Research Center
2016-2025
Bhabha Atomic Research Centre
1999-2004
Cornell University
1994-1996
When a fistulated cow was fed an all forage diet, ruminal pH remained more or less constant (6.7 to 6.9). The of concentrate-fed decreased dramatically in the period soon after feeding, and as low 5.45. Mixed bacteria from forage-fed converted CO2 H2 methane, but fluid did not produce methane. adjusted 7.0, methane eventually detected, absolute rate production high one observed with (0.32 h−1). Based on zero-time intercepts production, it appeared that had fewer methanogens than cow. mixed...
We describe a method that adds long-read sequencing to mix of technologies used assemble highly complex cattle rumen microbial community, and provide comparison short read-based methods. Long-read alignments Hi-C linkage between contigs support the identification 188 novel virus-host associations determination phage life cycle states in community. The assembly also identifies 94 antimicrobial resistance genes, compared only seven alleles short-read assembly. demonstrate techniques work...
Antibiotic growth promoters (AGPs) are frequently used to enhance weight-gain in poultry production. However, there has been increasing concern over the impact of AGP on emergence antibiotic resistance zoonotic bacterial pathogens microbial community gut. In this study, we adopted mass-spectrophotometric, phylogenetic, and shotgun-metagenomic approaches evaluate bioactive phenolic extracts (BPE) from blueberry (Vaccinium corymbosum) blackberry (Rubus fruticosus) pomaces as alternatives...
Unpasteurized dairy products are known to occasionally harbor Listeria monocytogenes and have been implicated in recent listeriosis outbreaks numerous sporadic cases of listeriosis. However, the diversity virulence profiles L. isolates recovered from these not fully described. Here we report a genomic analysis 121 milk, milk filters, milking equipment collected bovine farms 19 states over 12-year period. In multi-virulence-locus sequence typing (MVLST) analysis, 59 Virulence Types (VT) were...
Denitrification N losses during manure net mineralizable assays may lead to miscalculation of the manure's N‐supplying capacity. In this study we measured denitrification, properties, gas fluxes, nutrient pools, and laboratory incubation manured soil. Different dairy manures ( n = 107) were added soil at a rate 0.1 mg g −1 Manured control soils incubated sampled weekly for mineral N, CO 2 flux, O flux. The denitrification enzyme activity (DEA) was end experiment. Weekly production increased...
ABSTRACT The objective of this study was to evaluate whether cows that were low shedders Mycobacterium avium subsp. paratuberculosis passively shedding or truly infected with M. . We also investigated it is possible these -infected animals could have been as adults by contemporary high-shedding (supershedders). isolates obtained from a longitudinal three dairy herds in the northeastern United States. Isolates selected fecal samples and tissues at slaughter all culture positive same time...
Raoultella planticola is an emerging opportunistic pathogen closely related to members of the Klebsiella genus. Genomic characterization R. isolates recovered from food animals lacking. Here we describe antimicrobial resistance genes, virulence factors, plasmid replicons, and phylogeny three antimicrobial-resistant feces dairy calves within 48 hours birth. The genomes were sequenced on a NextSeq 2000 platform using paired-end sequencing. raw reads trimmed Trimmomatic assembled SPAdes v...
A longitudinal study aimed to detect Listeria monocytogenes on a New York State dairy farm was conducted between February 2004 and July 2007. Fecal samples were collected every 6 months from all lactating cows. Approximately 20 environmental obtained 3 months. Bulk tank milk in-line filter weekly. Samples milking equipment the parlor environment in May Fifty-one of 715 fecal (7.1%) 22 303 (7.3%) positive for L. monocytogenes. total 73 108 (67.6%) 34 172 bulk (19.7%) isolated 40 (15%)...
Salmonella enterica subsp. serovar Kentucky is frequently isolated from healthy poultry and dairy cows occasionally people with clinical disease. A genomic analysis of 119 isolates collected in the United States cows, ground beef, products, human cases was conducted. Results demonstrated that majority bovine-associated S. were sequence type (ST) 152. Several (n = 3) food product ST198, a Northern Africa, Europe Southeast Asia. phylogenetic indicated both STs are more closely related to other...
Preweaned dairy calves and lactating cows are known reservoirs of antibiotic-resistant bacteria. To further understand the differences in resistomes microbial communities between two, we sequenced metagenomes fecal composite samples from preweaned on 17 commercial farms (n = 34 samples). Results indicated significant structures (analysis similarities [ANOSIM] R 0.81, p 0.001) (ANOSIM 0.93 to 0.96, two age groups. Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria were predominant...
Abstract Salmonella Kentucky is among the most frequently isolated S. enterica serovars from food animals in United States. Recent research on isolates recovered these suggests there may be geographic and host specificity signatures associated with S . strains. However, sources genomic features of human clinical States remain poorly described. To investigate characteristics possible infections, genomes all cases State Maryland between 2011 2015 ( n = 12) were sequenced compared to a database...
Food animals are known reservoirs of multidrug-resistant (MDR) Escherichia coli , but information regarding the factors influencing colonization by these organisms is lacking. Here we report genomic analysis 66 MDR E . isolates from non-redundant veal calf fecal samples. Genes conferring resistance to aminoglycosides, β-lactams, sulfonamides, and tetracyclines were most frequent antimicrobial genes (ARGs) detected included those that confer clinically significant antibiotics ( bla CMY-2...
ABSTRACT Dairy farms are a reservoir for Listeria monocytogenes , and the reduction of this pathogen at farm level is important reducing human exposure. The objectives research were to study diversity L. strains on single dairy farm, assess strain dynamics within identify potential sources in bulk tank milk filters, adherence abilities representative strains. A total 248 isolates analyzed by pulsed-field gel electrophoresis (PFGE). Combined AscI ApaI restriction analysis yielded 40 PFGE...
Antimicrobial resistance has become a major global public health concern, and agricultural operations are often implicated as source of resistant bacteria. This study characterized the prevalence antimicrobial-resistant Salmonella enterica Escherichia coli from total 443 manure composite samples preweaned calves, postweaned dry cows, lactating cows 80 dairy in Pennsylvania. A 1095 S. 2370 E. isolates were screened tested for to 14 antimicrobials on National Resistance Monitoring System...
Enterohemorrhagic Escherichia coli (EHEC) are a physiologically, immunologically and genetically diverse collection of strains that pose serious water-borne threat to human health. Consequently, immunological PCR assays have been developed for the rapid, sensitive detection presumptive EHEC. However, ability these consistently detect EHEC while excluding closely related non-EHEC has not documented. We conducted 30-month monitoring study major metropolitan watershed. Surface water samples...
Listeria monocytogenes, the causative agent of listeriosis, is frequently isolated from environment. Dairy cows and dairy farm environments are reservoirs this pathogen, where fecal shedding contributes to its environmental dispersal contamination milk, products, meat. The molecular diversity 40 L. monocytogenes isolates representing 3 serogroups (1/2a, 1/2b, 4b) collected between 2004 2010 feces cattle on a single was assessed using multivirulence locus sequence typing (MVLST) assay. MVLST...
The gastrointestinal tracts of dairy calves and cows are reservoirs antimicrobial-resistant bacteria (ARB), which present regardless previous antimicrobial therapy. Young harbor a greater abundance resistant than older cows, but the factors driving this high unknown. Here, we aimed to fully characterize genomes multidrug-resistant (MDR) antimicrobial-susceptible Escherichia coli strains isolated from pre-weaned calves, post-weaned dry lactating identify accessory genes that associated with...
Gram-negative, ionophore-resistant ruminal bacteria and Gram-positive, ionophore-sensitive species bound similar amounts of [14C]lasalocid, but neither group large [14C]monensin. Membrane vesicles also more lasalocid than monensin (P < .05). The binding was first-order at low cell or vesicle concentrations saturable high densities. Streptococcus bovis inhibited by both (5 microM), cells that were re-incubated in medium lacking ionophore grew rapidly. Lasalocid-treated very slowly when they...