Common scab, a globally important potato disease, is caused by infection of tubers with pathogenic Streptomyces spp. Previously, disease-resistant somaclones were obtained through cell selections against the pathogen's toxin, known to be essential for disease. Further testing revealed that these clones had broad-spectrum resistance diverse tuber-invading pathogens, and was restricted tuber tissues. The mechanism enhanced disease not known. Tuber periderm tissues from their susceptible parent...

Spongospora subterranea is responsible for significant potato root and tuber disease globally. Study of this obligate (non-culturable) pathogen that infects below-ground plant parts technically difficult. The capacity to measure the dynamics patterns infections can greatly assist in determining efficacy control treatments on progression. This study used qPCR histological analysis time-course experiments temporal multiplication development (and tomato) roots tubers. Effects delayed initiation...

Light-induced tuber greening is one of the most important quality defects potato. Although varietal and maturity factors are known to affect resistance, physiological mechanisms resistance poorly understood. We proposed that biochemical within periderm provide hypothesised primarily related suberin content. investigated differences in between genotypes maturities varied propensity. examined light-induced pigment accumulation, phellem cell development studied propensity mutant chemically...

Multiple disease resistance is an aim of many plant breeding programs. Previously, novel somatic cell selection was used to generate potato variants “Russet Burbank” with common scab caused by infection actinomycete pathogen. Coexpression powdery a protozoan pathogen subsequently shown. This study sought define whether this effective against additional tuber diseases, black scurf, and soft rot induced fungal bacterial pathogens. Pot trials in vitro assays multiple pathogenic strains...

Variant somaclones of potato cultivar R usset B urbank, selected for resistance to common scab using in vitro cell selection techniques, were tested powdery scab, another important disease caused by S pongospora subterranea . This pathogen also invades roots, producing root galls. Most variants consistently showed increased both field and glasshouse challenge, when compared the parental cultivar, several significantly so. On average, best variant reduced incidence 51% severity (tuber surface...

Spongospora subterranea f. sp. is an important pathogen of potato responsible for major losses in most growing regions the world. Infection initiated by biflagellated motile zoospores released from long-lived resting spores. Zoospore chemotaxis to host plant root widely believed be stimulated exudate compounds, although direct evidence lacking. This study refined traditional capillary assay, with which we provided first empirical S. zoospore chemotaxis. Individual metabolites were either...

Light conditions in retail stores may contribute to potato greening. In this study, we aimed develop a tuber greening risk rating model for based on light quality and intensity parameters. This was achieved by firstly exposing three varieties (Nicola, Maranca Kennebec) seven specific wavelengths (370, 420, 450, 530, 630, 660 735 nm) determine the propensity. Detailed measurements from 25 were then combined with propensity data model. Our study showed that maximum occurred under blue (450...

The genus Polerovirus contains positive-sense, single-stranded RNA plant viruses that cause significant disease in many agricultural crops, including vegetable legumes. This study aimed to identify and determine the abundance of species present within Tasmanian pea crops surrounding weeds may act as virus reservoirs. We further sought examine genetic diversity TuYV, most commonly occurring polerovirus identified. Pea weed samples were collected during 2019-2020 between October January from...

Ca2+ signaling regulates physiological processes including chemotaxis in eukaryotes and prokaryotes. Its inhibition has formed the basis for control of human disease but remains largely unexplored plant disease. This study investigated role on motility Spongospora subterranea zoospores, responsible root infections leading to potato tuber Cytosolic flux with antagonists were found alter zoospore swimming patterns constrain chemotaxis, attachment zoosporangia infection. LaCl3 GdCl3, both...

Brassica yellows virus (BrYV), a tentative species in the genus Polerovirus, of Solemoviridae family, is phloem-restricted and aphid-transmitted with at least three genotypes (A, B, C). It has been found across mainland China, South Korea, Japan. BrYV was previously undescribed Tasmania, its genetic variability state remains unknown. Here, we describe near-complete genome sequence (genotype A) isolated from Raphanus raphanistrum Tasmania using next-generation sequencing sanger RT-PCR...

Downy mildew is a serious threat to opium poppy production globally. In recent years, two pathogen species, Peronospora somniferi and meconopsidis, which induce distinct symptoms, have been confirmed in Australia. order manage the spread of these pathogens, identifying sources inoculum essential. this study, we assessed presence associated with seed. We developed PCR qPCR assays targeting coxI coxII gene regions, for detection, differentiation, quantification P. meconopsidis These results...

Abstract Spongospora subterranea , which causes powdery scab of potato, infects a diverse range plant species. Crop rotation as management tool will be compromised if pathogen hosts exist between potato crops. Opium poppy ( Papaver somniferum ) and pyrethrum Tanacetum cinerariifolium are important crops within intensive vegetable production rotations in NW Tasmania. Measurements S. soil inoculum commercial field showed amounts were substantially elevated following an opium crop, suggested...

Downy mildew of opium poppy is the single biggest disease constraint afflicting Australian industry. Within pathosystem, transmission infections via infested seed major concern. Both downy pathogens poppy; Peronospora meconopsidis and P. somniferi, are known contaminants commercial stocks. Using naturally with these pathogens, effect physio-chemical treatments on seedling health were evaluated. Individual tested to determine optimal treatment parameters for each; including incubation time,...